Okolab H301-T-UNIT-BL-PLUS, Руководство пользователя

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2. Insert the Fine Gauge Thermocouple into the chamber by threading the wire through one of the
screw holes on the chamber riser.
3. Secure the end of the Fine Gauge Thermocouple to the bottom of Petri dish using some tape making
sure to avoid covering the head of the probe (see Figure 49).
4. Fill the Petri dish with distilled water to a level sufficient to fully immerse the Fine Gauge
Thermocouple. This is a Reference Well simulating experimental conditions. Place the lid on the
Petri dish, and insert it in the incubation chamber. You can also use SENSOR LID-# to close the dish
(see the “ Tip ” in paragraph 7).
Note ► Please, monitor the room temperature during the calibration and reduce air drafts and forced
convection. If possible divert any draft coming from the air conditioning system in the room, from the
incubation system.
5. Wait until the system reaches the steady state (temperature indicator status is green) then approach
the oil immersion objective to the bottom of the Petri dish. The Fine Gauge Thermocouple will detect
a decrease in temperature because of the contact with the cold lens. You can see the Fine Gauge
Thermocouple temperature decreasing in the Status page (see paragraph Figure 37) whether you are
working in “Chamber Feedback Mode” or in “Sample Feedback Mode
If you are working in “Chamber Feedback Mode”, verify that the Chamber Calibration has
already been completed. Or alternatively, that the factory calibration is appropriate for your
laboratory room temperature (i.e. that, even in Chamber Mode, the sample is close to the
Set Point temperature). If you are unsure it is recommended to re-check the room
temperature and decide whether to re-calibrate the chamber (see 6.2.1.2) and then the
Objective Heater.
6. When the Fine Gauge Thermocouple reads a stable temperature open the “ Objective Heater ” p age
as shown in Figure 20 and select “Use Manual” (see Figure 22). Next click on “+” or “ - ” on the
“ Offset ” tab (see Figure 22) to adjust the Objective Heater offset value. Okolab recommends starting
with an offset value corresponding to the difference between the set point temperature and the
actual temperature measured by the Fine Gauge Thermocouple. Press “ S et” and wait until the
temperature measured by the Fine Gauge Thermocouple is stable. If the temperature measured by
the Fine Gauge Thermocouple is now close to the set point, then the Manual Calibration is complete.
If the temperature measured by the Fine Gauge Thermocouple is far from the set point, repeat the
steps above until the temperature measured by the Fine Gauge Thermocouple reaches the set point
temperature