37
Before starting the Objective Heater Self-Calibration carefully read the instructions given in
this paragraph and in paragraph 6.2.1.3.
The goal of the Self-Calibration procedure is to adjust the Objective Heater temperature by
automatically
varying its offset; while maintaining the sample at the desired temperature (e.g. 37.0 °C) once the oil
immersion objective is in contact with the bottom of the well. Before starting the Objective Heater Self-
Calibration:
1.
Plug the green Fine Gauge Thermocouple in the port labeled
“
Reference T
emperature” located in
the rear panel of the Temperature Control Unit, as illustrated in Figure 2.
2.
Insert the Fine Gauge Thermocouple into the chamber by threading the wire through one of the
screw holes on the chamber riser.
3.
Secure the end of the Fine Gauge Thermocouple to the bottom of Petri dish using some tape making
sure to avoid covering the head of the probe (see Figure 49).
4.
Fill the Petri dish with distilled water to a level sufficient to fully immerse the Fine Gauge
Thermocouple. This is a Reference Well simulating experimental conditions. Place the lid on the
Petri dish, and insert it in the incubation chamber. You can also use SENSOR LID-# to close the dish
(see the “
Tip
” in paragraph
7).
Note
►
Please, monitor the room temperature during the calibration and reduce air drafts and forced
convection. If possible divert any draft coming from the air conditioning system in the room, from the
incubation system.
5.
Wait for until the system reaches the steady state (temperature indicator turns green) and then:
A.
Chamber Feedback Mode
:
Enable the Objective Heater as shown in Figure 20. Follow the instructions on the message: first you
have to bring your immersion lens in contact with the Petri dish, next press
“CALIBRATE”
.
Following
this sequence of instructions REQUIRED to control the Objective Heater only, without interfering
with the incubation chamber
.
Once you have pressed
“Calibrate”
, the dialog shown in Figure 24 will open. If the Fine Gauge
Thermocouple is close to the Set Point Value, press
“Start”
, otherwise you have to calibrate the
Chamber Incubator first and then can calibrate the Objective Heater.
Make sure that Chamber Calibration is completed. If the Fine Gauge Thermocouple measures
a temperature far from the Set point Value pressing,
“Start”
will still launch the Self-
Calibration procedure. This is NOT advisable because the procedure will raise the offset
value of the objective heater.
B.
Sample Feedback Mode
:
Содержание H301-T-UNIT-BL-PLUS
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