20
Figure 17. Sample Mode setting
In this configuration, every time you run an experiment, you must place the Fine Gauge Thermocouple (thin
green wire) into a Petri dish or well adjacent to your sample (from here on this will be referred to as
Reference Well). Fill the Reference Well with distilled water to a level allowing full immersion of the fine
gauge thermocouple (see Figure 49). When possible close the petri with its own plastic lid (or with SENSOR
LID, see
Tip
in paragraph 7) and place it in the appropriate holder. Using a Reference Well in close proximity
to your Experimental Well provides active Sample Feedback Temperature Control. This ensures that your
sample will be within 0.1°C from the temperature set point regardless the room temperature variations.
Tip
►
Advantages of Sample Feedback Control Mode are: 1) direct monitoring and active control of sample
temperature and 2) independence from room temperature variations. The disadvantage is that it requires
manual operation for correct placement of the fine gauge thermocouple in a Reference Well.
The Control Mode page also includes an
Open Incubator
button
(see Figure 18). Press Open Incubator before
opening the lid of the Incubation Chamber (e.g. in order to add culture medium). This places the thermal
controller in stand-by and avoids overheating the sample.
Note
►
: this feature is available only when operating in
Sample Feedback Mode
Control.
Figure 18. Open Incubator
After closing the incubation chamber wait a couple of minutes and press
“Incubator Closed”. Now you can
continue your experiment.
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