Okolab H301-T-UNIT-BL-PLUS, Руководство пользователя

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7 C
ALIBRATIONS
Carefully read the instructions given in this paragraph and before starting the Chamber
Calibration.
Note
►
It is important to monitor the room temperature during the calibration, as well as reducing air
drafts and forced convection. If possible divert any draft coming from the air conditioning system in the
room, from the incubation system.
The goal of this procedure is to automatically adjust the temperature offset of the Base and Lid of the
incubation chamber hence maintaining the sample at the desired temperature (e.g. 37°C) during imaging.
Before starting the Chamber Self-Calibration:
1. Plug the green Fine Gauge Thermocouple in the port labeled “ Reference T emperature” located in
the rear panel of the Temperature Control Unit, as illustrated in Figure 2.
2. Insert the Fine Gauge Thermocouple into the chamber by threading the wire through one of the
screw holes on the chamber riser.
3. Secure the end of the Fine Gauge Thermocouple to the bottom of a 35mm or similar Petri dish using
some tape making sure to avoid covering the head of the probe (see Figure 49).
Figure 49. Free Sensor attached on the internal base of the Petri Dish
4. Fill the Petri dish with distilled water to a level sufficient to fully immerse the Fine Gauge
Thermocouple. This is a Reference Well simulating experimental conditions. Place the lid on the
Petri dish, and insert the dish in the incubation chamber. You can also use SENSOR LID-# to close the
dis h (see the “ Tip ” below).