User
Guide
for
Axoclamp
900A
CELL
mode—two
microelectrodes
connected
to
a
50
M
Ω
//
470
pF
cell.
The
case
of
the
model
cell
is
connected
to
ground.
Shielding
between
the
two
microelectrode
resistors
is
effected
by
the
body
of
the
switch.
When
the
switch
is
in
the
BATH
position,
both
microelectrode
resistors
are
connected
to
ground.
This
is
a
convenient
position
for
practicing
bridge
balancing
techniques
and
offset
adjustment.
When
the
switch
is
in
the
CELL
position,
both
microelectrode
resistors
are
effectively
intracellular.
In
Bridge
or
DCC
mode
you
should
see
exponential
voltage
responses
to
steps
of
current.
In
dSEVC
mode
you
should
be
able
to
clamp
the
cell
at
gains
of
up
to
0.8
nA/mV
using
an
HS
‐
9A
×0.1
headstage,
at
sampling
rates
up
to
8
kHz.
In
TEVC
mode,
use
one
of
the
following
microelectrode
headstage
combinations:
two
HS
‐
9A
×0.1
headstages,
two
HS
‐
9A
×1
headstages,
or
an
HS
‐
9A
×0.1
headstage
for
CHANNEL
1
and
an
HS
‐
9A
×1
headstage
for
CHANNEL
2.
Figure
3.
CLAMP
‐
1U
model
cell.
20
