Molecular Devices Axoclamp 900A, Руководство пользователя

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User Guide for Axoclamp 900A
should be able to get a rise time of about 100
μ
s, with a gain of about
350 to 400 and noise of less than 20 mV
p-p
(2 mV at the cell).
Oscilloscope channel 2 monitors current and should display a
waveform that settles in less than 200
μ
s.
Figure 28. Optimizing TEVC mode. IN0 is the membrane potential (×10). IN1
is the membrane current (1 V/
μ
A). The voltage
‐
clamp Gain is 388. Pipette
Capacitance Neutralization is 3.5 pF.
Oscillation Detection
1. In the
Detect oscillation
section of the TEVC tab, you can choose to
Reduce gain in 50% steps
or
Set Gain to minimum
.
Figure 29. Oscillation detection settings for voltage
‐
clamp modes
(TEVC, dSEVC).
2. Enable
Detect oscillation
.
3. Increase the gain until the amplifier oscillates. The following message
will appear when an oscillation is detected. This will typically happen
at gains between 1000 and 2000 in TEVC mode.
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