Installation
and
Basic
Operation
2.
When
the
Axoclamp
900A
receives
a
ʺ
non
meter
ʺ
command
it
flashes
ON
then
OFF.
3.
Toggle
the
Headstage
1
and
Headstage
2
Mode
buttons
in
the
Axoclamp
Commander,
switching
repeatedly
between
the
different
voltage
‐
clamp
and
current
‐
clamp
modes.
The
tabs
immediately
below
the
mode
switches
in
the
software
should
change
appropriately.
Also,
LED
indicator
lights
on
the
front
panel
of
the
Axoclamp
900A
should
change
with
the
different
modes.
The
green
I
‐
CLAMP
LEDs
should
light
for
IC
and
I=0,
a
yellow
LED
for
DCC,
a
red
LED
for
dSEVC,
and
a
blue
LED
for
HVIC.
For
TEVC
mode,
the
green
CHANNEL
1
I
‐
Clamp
LED
and
the
blue
TEVC
LED
should
light.
Check
to
see
that
the
LEDs
change
with
the
modes
selected
in
the
software.
4.
If
the
USB
cable
is
unplugged
the
STATUS
LED
flashes
briefly
ON
then
OFF.
Model
Cells
We
recommend
that
you
practice
using
the
Axoclamp
900A
with
model
cells.
The
CLAMP
‐
1U
model
cell
shipped
with
your
Axoclamp
900A
is
a
useful
tool
for
learning
the
operation
of
the
Axoclamp
900A
and
subsequently
for
verifying
the
correct
operation
of
the
Axoclamp
900A
and
the
recording
pathway.
The
CLAMP
‐
1U
Model
Cell
The
cell
and
microelectrode
components
simulate
a
medium
sized
cell
having
an
input
resistance
of
50
M
Ω
,
a
membrane
time
constant
of
~25
ms
and
microelectrode
resistances
of
50
M
Ω
.
A
switch
allows
the
CLAMP
‐
1U
model
cell
to
be
configured
as:
BATH
mode—two
50
M
Ω
microelectrodes
to
ground.
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