30
Operation
BlueShadow Pump 80P Instructions V7685
Upon switch-on: The rear piston area of the pump head is automatical-
ly flushed for 15 seconds.
In continuous mode: The rear piston area of the pump head is flushed
automati cally every 30 minutes for 15 seconds.
Select a suitable solvent in the backpiston flushing. The solvent used
for the flushing depends on the application and the used solvent in the
pump:
Solvent in the pump
Piston backflushing
Reverse phase solvents
50% isopropanol or ethanol with
50% water (v/v)
Normal phase solvents
100% isopropanol
Buffers with high salt
concentrations
Rinse with water containing
5% ethanol or isopropanol
Legend
1
Inlet from the solvent
bottle to the flush
pump
2
Outlet from the flush
pump to the pump
head
Fig. 17:
Connectors for piston backflushing
5.4 Switch on and self test
1.
Switch on the pump.
2.
The display shows
Initialization. The device runs a self test.
3.
Wait until the self test has been completed.
4.
After the test has been successfully completed, the most recently ac-
tive program will be displayed.
5.5 Operating with LC display and keypad
The LC display operation of the pump is suitable for the following tasks:
Monitoring device functions.
Using special programs for laboratory work:
—
Flushing program
—
Standby and Wake up (WU) program
—
Program sequences (
Link menu)
—
Checking the system status as part of quality assurance measures
in accordance with good laboratory practices (
GLP menu).
Recommended
purging solution
Procedure
1
2