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User Manual 3.4

INTEGRA Biosciences

Page 1

ENGLISH

1

MediaClave overview

1.1

About the MediaClave

1.1.1

Introducing the MediaClave

Figure 1.1: The MediaClave

1.1.2

Intended use

The MediaClave is a highly versatile piece of equipment. It operates as a stand-alone device used to pro-
gram and control three integrated operation modes: MEDIACLAVE, AUTOCLAVE and WATERBATH.
The MEDIACLAVE program is used to prepare culture media. Insertion of a stainless steel base plate and
flexible PT100 temperature probe converts the MediaClave into the AUTOCLAVE. The AUTOCLAVE pro-
gram is used as a bench-top autoclave for sterilisation of culture media in single containers. The WATER
BATH program is used as a conventional water bath.

NOTE: 

Consult section 7.2.1 for a list of all standard and optional MediaClave accessories.

Safety Cover Lock

Safety Valve

Vessel Lid

Safety Cover

Outlet Cap

Inlet Cap

Form Feed Button

Printer

Manometer

Magnetic Front Panel

Graphic User
Interface (GUI)

Содержание MediaClave

Страница 1: ...User Manual 3 4 Bedienungsanleitung 3 4 Mode d emploi 3 4...

Страница 2: ...for measurement control and lab oratory use General requirements EN 61010 2 041 1998 Particular requirements for autoclaves using steam for the treatment of medi cal materials and for laboratory proc...

Страница 3: ...uction 7 2 2 Operating environment 7 2 3 MediaClave drawings 8 2 3 1 MediaClave vessel overview 8 2 3 2 MediaClave rear panel 9 2 4 Electricity and water 10 2 4 1 Provision of electricity 10 2 4 2 Wat...

Страница 4: ...lave Cleaning menu 39 5 3 1 Cleaning procedure 39 5 3 2 Overview 39 5 3 3 EMPTY menu 39 5 3 4 CLEAN menu 40 6 Maintenance 43 6 1 Introduction 43 6 2 Paper handling 43 6 2 1 Replacing the Printer paper...

Страница 5: ...mit values within mode AUTOCLAVE 55 7 5 4 Limit values within mode WATERBATH 55 7 6 Input Output connectors 56 7 6 1 Overview 56 7 6 2 First serial port RS232 56 7 6 3 Second serial port RS232 57 7 6...

Страница 6: ...Table of contents INTEGRA Biosciences PAGE IV...

Страница 7: ...ogram is used to prepare culture media Insertion of a stainless steel base plate and flexible PT100 temperature probe converts the MediaClave into the AUTOCLAVE The AUTOCLAVE pro gram is used as a ben...

Страница 8: ...raceability of individual instrument functions and programmed processes progress printouts via the integrated printer Adjustment of the graphic user interface GUI to suit the laboratory environment Ea...

Страница 9: ...libration Paper handling 7 Accessories and Technical specifications Accessories default values ports physical properties 8 Manufacturer and Customer service Addresses 9 Glossary Term definitions Table...

Страница 10: ...lace safety must also be observed These installation and operating instructions must at all times be readily available where the Media Clave is used The MediaClave must not be used for any purpose apa...

Страница 11: ...ate collection and recycling For more information about where you can drop off your waste equipment for recycling please contact your local dealer from whom you originally purchased the product or you...

Страница 12: ...MediaClave overview INTEGRA Biosciences Page 6...

Страница 13: ...must be positioned in such a way that the On Off and power switches are always accessible In case of voltage fluctuations the INSTALLATION CATEGORIES Overvoltage Categories l ll or lll are authoritat...

Страница 14: ...2 3 MediaClave drawings 2 3 1 MediaClave vessel overview Figure 2 1 Vessel lid PT100 Temperature Probe Product Sensor Grip Vessel Lid Safety Valve Filling Port Safety Cover Lock Dispense Port Grip Ve...

Страница 15: ...MediaClave rear panel Figure 2 3 Waste tube of flow water outlet For Service Use only Product Sensor Host RS232 Vessel Cover and External Alarm RS232 Power Supply Hot Hot Drain Cold Water Flow Water O...

Страница 16: ...water outlet tube to the cooling water outlet Connect the waste tube for condensation and water vapour to the flow water outlet WARNING The MediaClave is an electrically heated pressure vessel and mus...

Страница 17: ...nut 2 6 Moving the MediaClave 2 6 1 Moving procedure Remove any items from the top of the MediaClave Close vessel lid and safety cover Disconnect all cables and tubing ATTENTION Replace the sterile f...

Страница 18: ...MediaClave overview INTEGRA Biosciences Page 12...

Страница 19: ...underside of the vessel lid s dispense port see figure 2 1 2 Attach the tube to the vessel lid by rotating the stainless steel securing nut clockwise until it is finger tight 3 2 1 3 Installing the cu...

Страница 20: ...lid anticlockwise until it unlocks and is released 3 Open the vessel lid 4 Remove the cuvette Take care about the product temperature sensor NOTE If insufficient water is added an alarmed error messag...

Страница 21: ...teel base plate 1 Remove the cuvette 2 Place the steel base plate into the vessel flat side facing up 3 2 2 3 Filling the vessel In mode AUTOCLAVE fill the vessel with distilled or deionised water to...

Страница 22: ...Removing of autoclaved material 1 Once the AUTOCLAVE cycle is finished press END to open the safety cover 2 Using both grips turn the vessel lid anticlockwise until it unlocks and is released 3 Open...

Страница 23: ...a self test is per formed On successful completion of initialisation and the self test the safety cover automatically opens and the start screen appears see figure 3 2 3 3 1 MediaClave start display T...

Страница 24: ...tions 3 3 2 2 MAIN menu options In the Main menu programs can be directly started or modified in the PROGRAM menu Choose one of the three main operation modes or programs MEDIACLAVE AUTOCLAVE or WA TE...

Страница 25: ...o reject all modifications and return to the PROGRAM menu Press to return to the previous menu options Figure 3 7 EDIT menu options 3 3 3 LANGUAGE menu 3 3 3 1 LANGUAGE menu options For selecting a la...

Страница 26: ...T 3 Press CONTINUE to scroll to and select PROGRESS PRINTOUT 4 Press or to select one of the following TABLE OFF 5 Press CONTINUE to select PRINT CYCLE 10 20 50s 1 2 10min default 1min 6 Press or to a...

Страница 27: ...ARAMETER ENTRY menu press CONTINUE to scroll to and select CONTRAST 2 Press EDIT 3 The SET CONTRAST menu appears 4 Press or to adjust the contrast to suit the working environment 5 Press SAVE to retur...

Страница 28: ...enu appears 4 Press or to adjust the cleaning temperature 5 Press SAVE to return to the PARAMETER ENTRY menu DATE 1 From the PARAMETER ENTRY menu press CONTINUE to scroll to and select DATE 2 Press ED...

Страница 29: ...R ENTRY menu INFO 1 From the PARAMETER ENTRY menu press CONTINUE to scroll to and select INFO 2 Press EDIT 3 The INFORMATION menu displays the following information VERSION software version SN device...

Страница 30: ...MediaClave overview INTEGRA Biosciences Page 24...

Страница 31: ...edge of the vessel ATTENTION Prior to each run remove the rubber lid seal wet the seal well and insert it again The ap propriate temperature sensor must be installed for sterilisation see section 3 2...

Страница 32: ...has been reached This allows water vapour to escape from the system thus all non sterile air is removed If the target value is below 100 C the vent valve closes at target value minus 3 C 4 3 1 5 Ster...

Страница 33: ...d clean the outlet tube immediately see section 5 2 PROGRAM 14 Without pressure test results in pressure free steam disinfection of growth media As the name suggests a pressure test is not performed a...

Страница 34: ...L to reject all modifications and return to the PROGRAM menu 15 Press START to start the selected program see Section 4 3 1 10 PROGRAM 15 Chocolate Agar 1 Follow steps 1 12 in section 4 3 1 9 PROGRAM...

Страница 35: ...f the media outlet 8 Close the vessel lid see section 3 2 9 Press OK to continue the cycle OR Press END to terminate the cycle 10 Close the safety cover PREPARE COOLING appears on the GUI All sensors...

Страница 36: ...t is printed every x seconds minutes during the sterilisation cycle x refers to the selected print cycle see section 3 4 4 2 NOTE The cycle progress is monitored and periodically updated on the GUI NO...

Страница 37: ...t valve remains open until the air vent parameter has been reached This allows water vapour to escape from the system thus all non sterile air is removed If the target value is below 100 C the vent va...

Страница 38: ...n to the EDIT menu where the program can be modified see section 3 4 2 4 3 Press CONTINUE to scroll to and select the sterilisation temperature 4 Press or to adjust the sterilisation temperature 5 Pre...

Страница 39: ...emove any trapped air WATER CONTROL appears on the screen The water level is checked PRESSURE TEST appears on the screen The sterilisation cycle is started 4 3 2 10 Flow of the AUTOCLAVE sterilisation...

Страница 40: ...value Figure 4 3 illustrates the progress of the WATER BATH cycle as displayed on the WATER BATH PROG screen Figure 4 3 The WATER BATH PROG screen NOTE The actual and target temperatures are displaye...

Страница 41: ...e 4 Press or to adjust the temperature 5 Press CONTINUE to scroll to and select the stirrer speed 6 Press or to adjust the stirrer speed 7 Press 8 Press SAVE to save all modifications and return to th...

Страница 42: ...heating water in the vessel ATTENTION Pressing END results in the following alarmed screen message CYCLE IS BEING CON CLUDED The MAIN menu appears ATTENTION Install the cuvette see section 3 2 and fi...

Страница 43: ...timal and safe operation of the MediaClave 5 2 1 1 Materials required The following materials are required Liquid dish washing detergent use a mixture of one part liquid dishwasher detergent and three...

Страница 44: ...washing detergent and three parts water Deionised water Soft lint free cleaning cloth 5 2 2 2 Safety Precautions 5 2 2 3 Cleaning procedure 1 Moisten a cleaning cloth with the liquid dish washing det...

Страница 45: ...tents are pumped out until the water level falls below the empty level sensor After the sensor switches off the pump the pump pauses for approximately 10 seconds The pump restarts repeatedly and pumps...

Страница 46: ...for 100 seconds and then expelled Emptying occurs in the same manner as in the EMPTY Menu see section 5 3 3 5 3 4 2 Cleaning the MediaClave 1 Open the safety cover and vessel lid 2 Press CLEAN on the...

Страница 47: ...following alarmed message appears TOO LITTLE HEATING WATER IN VESSEL Press END to confirm this error NOTE The actual and target temperatures are displayed in the upper right of the screen The pre prog...

Страница 48: ...MediaClave overview INTEGRA Biosciences Page 42...

Страница 49: ...c front cover 3 Press the form feed button to expel the last piece of paper 4 Remove the empty bobbin and replace with a new paper roll 5 Insert the end of the paper into the paper slit 6 The paper is...

Страница 50: ...MediaClave overview INTEGRA Biosciences Page 44 Figure 6 1 The MediaClave printer Figure 6 2 Inserting the Printer paper roll...

Страница 51: ...tire system Order number 135800 6 3 3 1 Using the INTEGRA temperature calibration set Order Nr 135800 Connect the double Pt100 sensor to the temperature sensor inlet There are two connector cables One...

Страница 52: ...is open Vessel lid is open Media inlet outlet caps are not sufficiently tight Check all settings Close safety cover Close vessel lid Manually tighten caps Insufficient or no pressure buildup during t...

Страница 53: ...NTION All alarmed errors are accompanied by an acoustic alarm This alarm can be switched off by pressing the END soft key Displayed Text Description Action DEVICE SAFETY PLEASE CALL CUSTOMER SERVICE D...

Страница 54: ...diaClave only within the specified environment conditions WARNING ICE IN THE SYSTEM PLEASE CHECK SENSORS INTERRUPTION TEMPERATURE SENSOR Description The MediaClave verifies before and during operation...

Страница 55: ...ty cover lock become warm during normal operation The bolt is magnetically operated and releases only if the cover is closed The magnet runs when the cover is open and so produces heat Thus this is a...

Страница 56: ...MediaClave overview INTEGRA Biosciences Page 50...

Страница 57: ...132175 Tubing securing nut for filling tube 132165 Magnetic stirrer beam complete 132130 Stainless steel cuvette complete 132125 Paper roll for thermo printer 135155 x Sterile filter 0 45um 132140 x D...

Страница 58: ...protective earth PE wire is required NOTE Mains voltage tolerance 10 Region Mains voltage VAC Phases min current A Wattage kW Frequency Hz Article Number Europe 3 x 400 3L N PE 6A per Ph 3 5 50 60 13...

Страница 59: ...spensing temperature 30 80 C 50 C Stirrer speed 1 2 1 75 rpm Table 7 4 MEDIACLAVE program 14 NOTE Blood is added to the sterilised medium and held for 10 minutes at 100 C there is no sterilisation of...

Страница 60: ...minute off Communication Device address Host Port 1 9600 Bd 1S 8D NP 1S Unit of measure Degrees Celsius Contrast 77 Sterilisation Mediaclave tolerance Autoclave tolerance 1 5 C 1 5 C Ventilation Vent...

Страница 61: ...14 Without pressure test Heat treatment temperature Heat treatment duration Dispensing temperature 70 105 C 1 99 minutes 30 80 C Program 15 Chocolate Agar Sterilisation temperature Sterilisation dura...

Страница 62: ...32 serial interface for communication with a PC Table 7 11 lists the presettings for the RS232 serial interface and table 7 12 details the interface for the external safety cover lock switch and buzze...

Страница 63: ...135245 is installed in the MediaClave MediaClave instruments which are purchased after January 2006 have this cable built in by default The Epson TM U220B is a matrix pro tocol printer Serial interfa...

Страница 64: ...tery link cover External alarm Battery link cover Table 7 14 Interface for external safety cover lock and alarm ATTENTION The buzzer built in voltage may amount to 24 V DC The contact is in an open st...

Страница 65: ...ol of the single program processes MEDIACLAVE AUTOCLAVE WATER BATH 7 7 2 Test print 7 7 2 1 Test print sample TEST PRINT RES 2 H 0 L 0 Y 0 RES 1 E 0 P 0 S 0 SWV 1 8 3 Date 15 03 2004 Time 12 38 Item p...

Страница 66: ...Serial number Software version All sensors are ok Vessel lid is closed Safety cover is closed Cooling system is checked Sufficient coupling medium available Pressure test passed Program Phases Heating...

Страница 67: ...ing temperature display Digital display in increments of 0 1 C Quality of steel used 1 4301 1 4305 Feed tube dimensions Internal diameter 6 mm Thickness 2 mm Product live span Max 10 000 sterilization...

Страница 68: ...MediaClave overview INTEGRA Biosciences Page 62...

Страница 69: ...facturer INTEGRA Biosciences AG Tardisstrasse 201 CH 7205 Zizers Switzerland www integra biosciences com info integra biosciences com 8 2 Customer Service Please contact your local INTEGRA Biosciences...

Страница 70: ...MediaClave overview INTEGRA Biosciences Page 64...

Страница 71: ...are prepared when using the MEDIA CLAVE programs This also functions as a water bath when using the WATERBATH programs For operation the cuvette has to be placed into the vessel DEIONIZED WATER Water...

Страница 72: ...emperature safety device used in temperature control circuit It is located at the base of the MediaClave PARAMETER MENU Screen at which users may view or modify 11 instrument parameters for each progr...

Страница 73: ...TERBATH CLEANING LANGUAGE and PARAMETER STEP This program function enables the operator to interrupt a cycle phase manually and proceed to the next phase in the program cycle This cannot be used durin...

Страница 74: ...MediaClave overview INTEGRA Biosciences Page 68...

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