IBS MediaClave, Руководство пользователя

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User Manual 3.4
INTEGRA Biosciences Page 31
ENGLISH
The safety cover opens automatically
11. Dispense the product (see section 4.3.1.7)
12. When the cuvette is empty, press
END . The cycle ends
4.3.2 AUTOCLAVE program operation
4.3.2.1 Overview
The flow of the AUTOCLAVE cycle is as follows:
System check
→
Pressure test
→
Heating
→
Sterilisation
→
Cooling
→
End of cycle
4.3.2.2 System check
All instrument functions and corresponding sensors are initialised and checked during a system check.
4.3.2.3 Pressure test
During a pressure test, the vent valve closes and the compressor operates for a maximum of 90 seconds.
When the target pressure of 0.1 bar is reached, the compressor stops and the vent valve re-opens.
4.3.2.4 Heating
The heating phase brings the product temperature to the programmed target value. To ensure the sterili-
sation of the head space inside the vessel, the vent valve remains open until the air vent parameter has
been reached. This allows water vapour to escape from the system, thus all non sterile air is removed. If
the target value is below 100°C, the vent valve closes at target value minus 3°C.
4.3.2.5 Sterilisation
The product is maintained at the programmed target value for the specified duration of the sterilisation
phase. The maximum sterilisation temperature is 122°C and the corresponding maximum pressure is ap-
proximately 1.2 bar. The sterilisation temperature runs within the defined tolerance band.
4.3.2.6 Cooling
The cooling phase reduces the temperature and pressure to the programmed target values. External cool-
ing water is flowing through the heat exchanger. The coupling medium also passes through the heat ex-
changer. The pump volume of 10 litres/minute and cooling performance of approximately 30 kW cools the
product to the required target value.
NOTE:
The actual and target dispensing times are displayed in the upper right of the screen.
ATTENTION:
Read section 7.4 before operating the AUTOCLAVE program.