GE AKTA avant, Руководство пользователя

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Do the cassettes in the fraction collector correspond to those selected in the method?
For more details, see Section 5.8.1 Prepare the built-in fraction collector, on page 203.
• Is there a risk that the number of wells in the deep well plate will not be sufficient for
the run? If this is the case, make sure that Last tube filled action is set to an appro-
priate action when creating the method (otherwise, the run will be paused). For more
details, see Online Help for the relevant method phase.
•
Have the Fraction collector F9-R and the built-in fraction collector been correctly
prepared? For more details, see relevant sections in Section 5.8.1 Prepare the built-
in fraction collector, on page 203 and Section 4.9 Fraction collector F9-R, on page 130.
• Are the inlet and inline filters clean or are they generating a back pressure higher
than normal? If this is the case, change the inline and inlet filters. For more details,
see Section 7.8.7 Replace the inlet filters, on page 348 and Section 7.3.2 Replace the
inline filter, on page 275.
•
Does the positioning of the columns correspond to the selections made in the
method? See Section 5.5 Connect a column, on page 182.
Purification checks
•
Have all columns been cleaned and prepared according to the column recommen-
dations?
•
Have the samples been adjusted to binding buffer conditions?
•
Have the samples been clarified by centrifugation and/or filtration prior to sample
loading?
•
Are the correct buffers used for the chosen columns and proteins?
•
Check buffers for precipitations. Adjust to room temperature.
•
Are the chosen columns suitable for the chosen target proteins?
•
Do the buffers have correct pH? The pH of some buffers changes with the tempera-
ture.
•
Are the UV-wavelengths used by the method appropriate with respect to used buffers
and proteins? For more details see GE Method Handbooks.
362 ÄKTA avant User Manual 29035184 AE
8 Troubleshooting
8.2 Troubleshooting: General Checklist