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5.7.1 Sample application using direct injection into the column
Introduction
There are two ways to load sample directly onto a column:
•
a fixed volume is loaded, or
•
all the sample is loaded.
Minimize sample loss
To minimize sample loss during direct injection of sample onto the column, sample re-
maining in the flow path will be pushed onto the column with buffer from the inlet valve.
This step is called Finalize sample injection in the text instructions of the sample appli-
cation phase of the method to be used. Refer to Section 5.9 Create a method and perform
a run, on page 225 for more information on methods and phases.
Then... When preparing to
inject...
•
manually prime the sample inlet tubing with sample,
see Prime sample inlets, on page 173.
•
in the Method editor, make the following selections
for the Sample Application phase of the method to
be run:
-
select Inject sample directly onto column,
-
select Inject fixed sample volume and set the
volume to be injected.
•
make sure that the flow path from the sample inlet
valve up to the injection valve will be filled with an
appropriate buffer:
-
make sure that the buffer inlet tubing of the inlet
valve is immersed in buffer, and
-
enable the function Wash sample flow path with
buffer in the Sample application phase.
a fixed volume of sam-
ple
ÄKTA avant User Manual 29035184 AE 191
5 Operation
5.7 Sample application
5.7.1 Sample application using direct injection into the column