25
4.
The pipettor is ready to perform according to the stored mode. Position the
tip to aspirate and press START button .
9. Pipetting
recommendations
The wide selection of different operating modes makes is possible to perform
several different liquid handling tasks with Biohit
e
LINE pipettors. The
operating modes P, P
+MIXING
, P
COUNT
, dd, dd+
MIXING
and SA feature an
automatic blow-out and others leave an excess liquid in the tip. The user should
follow the recommendations below to ensure optimal performance.
9. 1. Dispensing with blow-out
The automatic blow-out function, in different pipetting and diluting modes, is
followed by an immediate return of the piston to the home position. To avoid
accidental aspiration of the liquid back into the tip, it is recommended that the
dispensing is always performed above the liquid surface.
By holding the START button down during dispensing the piston will stop in the
lowest position. This allows the tip to be placed under the liquid surface or
against the bottom or the wall of the container during dispensing. Once the
liquid is dispensed, the tip can be removed from the container and the START
button released.
9.2. Dispensing without blow-out
The pipettor will not perform the blow-out function in modes rP, P
MANUAL
, d,
Ad, and Sd. Therefore it is recommended that dispensing in these modes is
always performed with the tip set against the wall or bottom of the container.
9.3. Other recommendations
-
Hold the pipettor vertically when aspirating the liquid and place the tip only
a few millimetres into the liquid.
-
Pre-rinse the tip before aspirating the liquid by filling and emptying the tip
for three to five times. This is important especially when pipetting liquids
with a viscosity and density greater than water.
-
Check that the pipettor, tip and liquid are at the same temperature.
-
To avoid the contamination, do not rest the pipettor on its side especially
when the tip attached.
-
Change the tip cone filter regularly.
-
Always place the pipettor in the charging unit when not in use.
Содержание eLINE 73002X
Страница 36: ...36 Notes...