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29

10.4. Storage

When  not  in use it is recommended that  the  pipettor is  always  stored in the 

e

LINE Charging Stand or Carousel. However, during long storage periods

(several months) it is recommended to disconnect the Charging Stand and
Carousel from the mains outlet.

It is also advisable to remove the battery pack from the pipettor. This protects 
the batteries against discharging during the long storage period.

11. Performance Testing

It is recommended to check the performance of your Biohit 

e

LINE pipettors

regularly (e.g. every 3 months) and always after in-house maintenance.
However, users should establish a regular testing routine for their pipettors
having regard to the accuracy requirements of the application, frequency of
use, number of operators using the pipettor, nature of the liquid dispensed and
the acceptable maximum permissible errors established by the user. (ISO 8655-1.)

Performance test should take place in a draught-free room at 15–30

O

C,

constant to ±0.5

O

C and relative humidity above 50%. The pipettor,  tips and the

test water should have stood in the test room sufficient time (at least 2 hours)
to reach equilibrium with the room conditions. Use distilled or de-ionised water
(grade 3). Use an analytical balance with a readability of 0.01 mgs.(ISO 8655-6.)

Weighting:

1. 

Adjust the desired test volume V

s

.

2.

Carefully fit the tip onto the tip cone.

3.

Fill the tip with test water and expel to waste five times to reach a humidity
equilibrium in the dead air volume.

4.

Replace the tip. Pre-wet the tip by filling it once with the test water and 
expel to waste.

5.

Aspirate the test water, immersing the tip only 2–3 mm below the surface
of the water. Keep the pipettor vertical.

6.

Withdraw the pipettor vertically and touch the tip against the side wall of 
the test water container.

7.

Pipette the water into the weighing vessel, touching the tip against the 
inside wall of the vessel just above the liquid surface at an angle of 30

O

to

45

O

. Withdraw the pipettor by drawing the tip 8–10 mm along the inner wall

of the weighing vessel.

8.

Read the weight in mgs (m

i

).

9.

Repeat the test cycle until 10 measurements have been recorded.

10. Convert the recorded masses (m

i

) to volumes (V

i

) by multiplying the mass

with the correction factor Z (Z-values in the table below): 
V

i

= m

i

*Z

Содержание eLINE 73002X

Страница 1: ...e pipetting rP 14 6 3 Pipetting with cycle counter P COUNT 15 6 4 Manual pipetting P MANUAL 16 7 MULTI modes 17 7 1 Multi dispensing d 17 7 2 Diluting dd and diluting with mixing dd MIXING 19 7 3 Sequ...

Страница 2: ...e keyboard and easy to read display together with user friendly operation and programming allows these tasks to be performed quickly and effortlessly The Biohit eLINE pipettors operate on the air disp...

Страница 3: ...rage with a plastic protector BEFORE USE PLEASE REMOVE THE PLASTIC PROTECTOR UNDER THE BATTERY COVER Note It is recommended to recharge the pipettor for at least two hours before initial use The pipet...

Страница 4: ...ousing Tip Ejector Bar Tip Cone s excl 5000 Safe Cone Filter Battery Cover Charging Contacts Reset Button Finger Hook Tip Cone e5000 Tip Ejector Assembly e5000 3 1 Display and controls The control and...

Страница 5: ...ction buttons Reset button Triggers all piston Press to remove tip Press to reset the movements pipettor Mode Ranges and Operating Mode Symbols PROG PROG 1 6 6 storage locations MULTI d multi dispensi...

Страница 6: ...piston will stop in the lowest position until the button is released In the operating modes with automatic blow out P dd the piston will automatically return to the home position after the start butt...

Страница 7: ...not have an adjustable tip ejector collar 3 5 Biohit Safe Cone Filters The tip cone design of the Biohit eLINE pipettors 10 l allows the use of Biohit Safe Cone Filters in the tip cones These removabl...

Страница 8: ...t eLINE Charging Stands and Carousels are bench top charging units The Charging Stand accommodates one Biohit eLINE pipettor and the Charging Carousel four Biohit eLINE pipettors The eLINE Charging St...

Страница 9: ...it is recommended to keep the pipettor in the Charging unit This ensures that the battery is kept charged and the pipettor is ready for use 4 2 Charging through direct charging system The AC adaptor s...

Страница 10: ...mode range is selected with the Select key the desired operating mode is then selected with the arrow key and selection confirmed with Enter key The setting changes volumes aliquots etc are made with...

Страница 11: ...change settings When pre stored including speed excluding speed Prog 1 6 was chosen Start pipetting Store the operating mode with Change selected settings with settings to Prog 1 6 Start pipetting P M...

Страница 12: ...udes 5 different operating modes 6 1 Pipetting mode P and pipetting with mixing P MIXING In pipetting mode P the eLINE performs pipetting with blow out In pipetting with mixing mode P MIXING pipetting...

Страница 13: ...ut and the piston returns automatically back to the home position Step 10 Step 11 Note When the START button is held down during dispensing the piston will stop in the lowest position When the start b...

Страница 14: ...7 Press or to confirm selection and to display the speed out 8 Select the speed out with 1 Min and 9 Max 9 Press or to confirm selections 10 Position the tip to aspirate and press the START button 11...

Страница 15: ...irm selection 6 Cycle counter will show 1 Use to select the sample number you want to begin with 0 99 7 Press to confirm the selection and to display the speed in or press and continue from Step 12 8...

Страница 16: ...is displayed 3 Confirm by pressing 4 Select the desired pipetting volume by using Note When holding down the volume display starts to scroll faster 5 Press to confirm selection and to display the spe...

Страница 17: ...dispensing of a selected volume The sum of the dispensing aliquots and an automatically selected excess volume is aspirated into the tip The excess volume is needed to ensure equal operating conditio...

Страница 18: ...own to indicate the reset function 13 Position the tip to discard priming excess and press the start button 14 To dispense position the tip press START button and repeat until the cycle is complete 15...

Страница 19: ...ing Note When holding down the volume display starts to scroll faster 5 Press or to confirm the selection 6 Select the desired sample volume volume 2 with 7 Press to confirm selection and to display t...

Страница 20: ...volumes of same solution can be delivered in any desired order The sum of dispensed volumes can not exceed the nominal volume of the pipettor 1 Press to display MULTI 2 Scroll with until the symbol Sd...

Страница 21: ...button twice Note It is also possible to continue to aspirate and dispense the same volumes without the empty function To continue keep the START button pressed during the last dispensing Step 16 and...

Страница 22: ...ete The total volume is displayed 13 Position the tips to dispense and press START button twice Step 12 Step 13 7 5 Automatic dispensing Ad The pipettor performs repetitive dispensing of a selected vo...

Страница 23: ...E OUT and arrow down to indicate the reset function 15 Position the tip to discard priming excess and press the START button 16 To dispense position the tip press the START button once and the pipetto...

Страница 24: ...g 1 6 locations 1 Complete the programming of the selected operating mode as described in paragraphs 6 and 7 2 Press first and then simultaneously to display the PROG 1 3 Choose the desired storage lo...

Страница 25: ...west position This allows the tip to be placed under the liquid surface or against the bottom or the wall of the container during dispensing Once the liquid is dispensed the tip can be removed from th...

Страница 26: ...ed always to use gloves when cleaning the pipettor Change the tip cone filter regularly with the tool provided 10 1 Cleaning the outer surface of the pipettor To clean and decontaminate the outer surf...

Страница 27: ...jector collar 2 tip cone holder 3 tip cone 4 spring 5 and piston 6 into a beaker containing Biohit Proline Biocontrol and leave for at least 30 minutes Fig 9 rinse the parts with distilled water Note...

Страница 28: ...pettor after in house service or maintenance see paragraph 11 10 3 Battery replacement The design of the Biohit eLINE pipettors enables fast and easy battery replacement The battery replacement pack i...

Страница 29: ...est water should have stood in the test room sufficient time at least 2 hours to reach equilibrium with the room conditions Use distilled or de ionised water grade 3 Use an analytical balance with a r...

Страница 30: ...ce between the dispensed volume and the selected test volume Random error is the scatter of the dispensed volumes around the mean of the dispensed volume ISO 8655 1 Note Biohit specifications are achi...

Страница 31: ...selected volume at a time as follows 1 Press to display PIPET 2 Scroll with until the desired mode P rP P MIXING or P COUNT is displayed 3 Confirm the selection by pressing 4 Select the pipetting volu...

Страница 32: ...action If any error message appears to the display it means that the pipettor has been unable to perform the attempted action properly In case of an error the display will show Error and PRESS Ent mes...

Страница 33: ...iston and the tip cone Insufficient amount of Grease accordingly grease on a piston and o ring Pipettor out of Instrument damaged Return to your established specifications Biohit service representativ...

Страница 34: ...nic pipettor is tested before shipping by the manufacturer The Biohit Quality Assurance Procedure guarantees that the Biohit eLINE electronic pipettor you have purchased is ready for use Each Biohit e...

Страница 35: ...8 ch 10 300 l 300 l 0 40 0 20 150 l 0 70 0 30 30 l 2 00 1 00 1 30 730390 8 ch 50 1200 l 1200 l 0 50 0 20 600 l 1 00 0 30 120 l 2 50 1 00 1 24 730420 12 ch 0 2 10 l 10 l 0 90 0 50 5 l 1 50 0 80 1 l 4...

Страница 36: ...36 Notes...

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