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13ACTEC®
9050 System User's Manual
4.5 Positive and Negative Specimens
4.5.1 General
Many positive cultures will be detected in the first 24 hours after inoculation,but
ongoing negative vials must still be kept for several days to assure maximum
recovery.With the
BACTEC® 9050
instrument, vials are typically held for 5 - 7
days before they are discarded as negative. Each laboratory should set the proto-
col length based on its own policies and conditions.Your protocol length can be
from 4 - 7 days.
A subculture and a Gram stain should be performed from each positive vial. In
most cases, organisms will be seen and a preliminary report can be made to the
physician. Preliminary antimicrobial susceptibility and identification procedures
may also be set up from fluid in the culture vials.The results from these prelimi-
nary tests should be confirmed by using standardized laboratory procedures.
The system allows re-entry of instrument-positive vials for up to 3 hours after
their removal (subject to the conditions described in Section 4.5.3).The re-entry
feature resets positivity routines, retains previous test readings, and continues to
test the vial as an ongoing culture.
For a maximum yield of isolates, negative cultures may be stained and/or subcul-
tured during the test period or immediately prior to their disposal.
4.5.2 Notification of Positive and Negative Vials
34
The system notifies you of new positive cultures in several ways:
♦
N e w Positive indicator lamp on the front of the instrument flashes.
♦
O n the Main Status display, the station is displayed as a filled circle with
a plus sign (+) in it, and the positives total in the Summary reflects the
number of positives in the instrument.
♦
W h e n the door is opened, the "remove positives" soft key appears in
the soft key assignment area of the LCD Display.
♦
U n t i l silenced, the audible alarm sounds (if enabled).
Negative cultures may exist as ongoing negatives (in protocol) and out-of-proto-
col negatives.You are notified of these conditions as follows:
♦
O n g o i n g Negatives - On the Main Status display, the station is dis-
played as a filled circle.
♦
O u t -of-Protocol Negatives - On the Main Status display, the station is
displayed as a filled circle with a minus sign (-) in it.Also, the negatives
count in the Summary reflects the number of final negatives in the
instrument. No audible alarm sounds for notification.When the door
is opened, the "remove negatives" soft key appears in the soft key as-
signment area of the LCD Display.
MA-0103-5
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