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BRISTOL-MYERS SQUIBB-LAWRENCEVILLE
LABORATORY PROCEDURE
TITLE: Bactec 9050
Automated Blood Culture
DATED: 3/13/98
AUTHOR: Trisha Cohen
REVIEW DATE:
N o r
OVERVIEW:
The detection of microorganisms in a patient's blood has diagnostic and prognostic
importance. Bacteria enter the blood from extravascular sites such as the genitourinary tract,
respiratory tract, abscesses, surgical wound infections and other miscellaneous sites either
directly or via the lymphatic vessels. When bacteria enter and/or multiply in the bloodstream at
a rate that exceeds the capacity of the reticuloendothelial system to remove them, bacteremia
results.
Blood cultures are essential in the diagnosis and treatment of the etiologic agents of sepsis.
Bacterial sepsis constitutes one of the most serious infectious diseases and therefore the
expeditious detection and identification of blood borne bacterial pathogens is an important
function of the clinical diagnostic laboratory..
The Bactec 9050 instrument is designed for the rapid detection of microorganisms in clinical
cultures of blood. The sample to be tested is inoculated into the vial which is entered into the
Bactec 9050 for incubation and periodic reading. Each vial contains a sensor which detects
increases in CO, produced by the growth of microorganisms. The sensor is monitored by the
instrument every ten minutes for an increase in its fluorescence, which is proportional to the
amount of CO, present. A positive reading indicates the presumptive presence of viable
microorganisms in the vial.
N4... M A T E R I A L S LIST:
A. Media
1. Bactec Peds Plus/F Culture Vial
Optimum blood volume for each vial is 1-5 mL.
Each vial contains:
a. 40mL Enriched Soybean-Casein digest broth.
b. 0.02% SPS
c. Resins
d. CO,
e. 0,
f. Sensor for CO, detection
2. Bactec Plus Anaerobic/F Culture Vial
Optimum blood volume for each vial 8-l0mL; 3-10 mL is acceptable
Each vial contains:
a. 25 mL pre-reduced enriched Soybean-Casein digest broth
b. 0.05% SPS
c. Resins
d. CO, and N, (which support the growth of anaerobic microorganisms)
e. Sensors for CO, detection
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