Pannoramic 250 FLASH III 3.0.2 User’s Guide
3.1 Preparing Slides and Magazines
Warning!
Ensure that no part of the mounted coverslip protrudes from the edges
of the slide. Use slides only with completely dried embedding medium.
If not so, the coverslip can be shifted that makes slide loading less
reliable. Not completely dried embedding might also influence the
operation of objectives and other device parts.
Caution!
Do
NOT
scratch any circular marker onto the glass surface of a slide,
use a soft tip pen instead for marking area of interest.
Note:
The distance between slide edges and the edges of the mounted
coverslip should be 1-2 mm, and they should be parallel with each
other.
Note:
Fluorescent signals become more easily detectable for the scanner,
therefore resulting in a brighter and better image, by treating the slide
with a formula that prevents rapid photobleaching of fluorescent
reagents (e.g. VECTASHIELD® Fluorescent Mounting Medium by
Vector
Laboratories
and ProLong® Gold and
SlowFade
® Gold antifade reagents
by
Invitrogen
) during the FISH protocol. For further information, see
the homepages of the manufacturers:
and
.
September 16, 2020 – Rev. 1
3DHISTECH Ltd.
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