YSI 2700 SELECT, User Manual

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2-5
Prepare the supply buffer and fill the buffer bottle. Powdered buffer concentrate is included in
the starter supplies. YSI 2357 is recommended for use with all YSI Enzyme Membranes except
YSI 2702 Galactose Oxidase membranes (use YSI 2705), YSI 2786 Ethanol Membranes (use
YSI 2787) and YSI 2725 Methanol Membranes (use YSI 1579). All YSI buffers may be ordered
separately.
» Place about 500 mL of reagent water (distilled and/or deionized) into a one liter flask,
or other clean container. Add two packages of YSI 2357 Buffer Concentrate and stir.
NOTE: YSI 1579 Carbonate Buffer Concentrate (used with 2725 membranes) must be
reconstituted as instructed on the bottle.
» Add more reagent water until the total volume of solution is between 900 and
1000 mL.
» Stir as necessary, until the buffer chemicals have completely dissolved.
» Disconnect the electrical lead from the level sensor and remove the bottle lid.
IMPORTANT: When adding fresh buffer to the Buffer Supply Bottle or when installing a new
bottle of Calibrator Solution, make every effort to avoid contamination of the lid and level
sensor assemblies.
» Pour the prepared buffer into the supply bottle, replace the lid, and reconnect the lead.
Install the calibrator solution. One or more of the following YSI calibration standards will be
provided in your starter supplies, YSI 2776 (Dextrose and L-Lactate), YSI 2780 (Sucrose), YSI
2783 (Lactose), YSI 2772 (Choline), YSI 2755 (L-Glutamate), YSI 2736* (L-Glutamine). YSI
calibrators may be purchased separately.
» Unplug the electrical lead from the level sensor in the lid of the empty calibrator bottle
and remove the lid.
»
Mark the date of installation on the new bottle of YSI calibrator solution.
» Screw the lid and level sensor assembly onto the new bottle and place it in the
instrument compartment. Reconnect the electrical lead.
* The YSI 2736 Glutamine Calibration Standard must be reconstituted as instructed on the
bottle. It is recommended that a sample be poured into a test tube and installed at station #2 for
calibration. Refrigerate the bulk solution when not in use.
IMPORTANT: The level sensor cables should not touch the instrument housing. It is best to
keep the cable connector on the bottle lid pointed toward the front. False messages concerning
fluid levels may result if the bottles are not installed inside the fluid compartment and the
cables routed as shown in Figure 2.3.
Figure 2.3
Bottles and Level Sensor Cables