YSI 2700 SELECT User Manual Download Page 151

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C-2

INSTRUMENT SETUP

----------------

Sample size

25 uL

Sample size

25 uL

SamStation #: 2

Cal Method: One station

BLACK PROBE

Chemistry

: Dextrose

Chemistry

: L-Lactate

Unit

: g/L

Unit

: g/L

Calibrator : 2.50

Calibrator : 0.50

End Point

: 30 Sec

End Point

: 30 Sec

CalStation# : 1

AUTOCAL

WHITE PROBE

SampleError : ON

Chemistry

: Dextrose

Temperature : 1 °C

Unit

: g/L

Time

: 15 Min

Calibrator : 2.50

Sample

: 5 Sam

End Point

: 30 Sec

Cal Shift

: 2 %

CalStation# : 1

RUN MODE

AUTOCAL

Replicates : OFF

SampleError : ON

Sample ID

: OFF

Temperature : 1 °C

Sip Height : Medium

Time

: 15 Min

Autostandby : 2 Hr

Sample

: 5 Sam

TT StartPos : 0

Cal Shift

: 2 %

TT # in Run : 0

RUN MODE

TT FluidDet : ON

Replicates : OFF

MONITOR

Sample ID

: OFF

MonStation# : 1

Sip Height : Medium

SamInterval : 0 Min

Autostandby : 2 Hr

PreCal

: 0 Min

TT StartPos : 0

PostCal

: 0 Min

TT # in Run : 0

Purge Time : 0 Sec

TT FluidDet : ON

RS-232

MONITOR

Baud rate

: 9600

MonStation# : 1

Data

: Seven bit

SamInterval : 0 Min

Stop

: One bit

PreCal

: 0 Min

Parity

: Even

PostCal

: 0 Min

Handshake

: RTS/CTS

Purge Time : 0 Sec

XON char

: 17

RS-232

XOFF char

: 19

Baud rate

: 9600

Mode : Non-multidrop

Data

: Seven bit

Address : 38

Stop

: One bit

GENERAL

Parity

: Even

Radix mark

: "."

Handshake

: RTS/CTS

Level sensor : ON

XON char

: 17

Cal Report

: Brief

XOFF char

: 19

SampleReport : Brief

Mode : Non-multidrop

DateFormat

: MM/DD/YY

Address: 38

Software revision: 2.56

GENERAL

YSI 2700S - 98 01234

Radix mark

: "."

Thu 02/26/98 09:20:48

Level sensor : ON

------------------------

Cal Report

: Brief

SampleReport : Brief
DateFormat

: MM/DD/YY

Software revision: 2.56
YSI 2700D - 98 01234
Thu 02/26/98 09:22:36
------------------------

Summary of Contents for 2700 SELECT

Page 1: ... Biochemistry Analyzer Who s Minding the Planet find out at ...

Page 2: ...al Setup Example Step by Step 2 15 2 10 Probe Baseline Check 2 25 3 1 Main Menu 3 1 3 2 Run Mode 3 2 3 3 Standby Mode 3 7 3 4 Daily Operational Checks 3 7 4 1 Principle 4 1 4 2 Sample Preparation 4 2 4 3 Measurement Parameter Setup Information 4 3 4 4 D Glucose Dextrose 4 5 4 5 L Lactate 4 6 4 6 Sucrose 4 7 4 7 Lactose 4 8 4 8 Ethanol 4 9 4 9 Starch 4 10 4 10 Choline 4 11 4 11 Galactose 4 12 4 12 ...

Page 3: ... 6 6 6 9 Software Structure 6 7 7 1 Daily Maintenance 7 1 7 2 Calibration Pumping System Maintenance 7 3 7 3 Sample Chamber Cleaning 7 4 7 4 Membrane Replacement 7 5 7 5 Probe Cleaning 7 6 7 6 Tubing Replacement 7 7 7 7 Sipper Replacement 7 9 7 8 Sipper Ground Cable 7 9 7 9 Fuse Replacement 7 10 7 10 Sipper Pump Seal Replacement 7 11 7 11 Sipper Mechanism Lubrication 7 13 8 1 Printout Information ...

Page 4: ...iii A 1 B 1 C 1 D 1 0 1 E 1 2 F 1 3 4 5 2 G 1 6 7 H 1 I 1 ...

Page 5: ...CT Fluid System 6 3 Figure 6 3 Typical Sensor Response 6 4 Figure 6 4 Aging Membrane Response 6 5 Figure 6 5 2700 SELECT Software Structure 6 7 Figure 7 1 Sample Chamber Illustration 7 5 Figure 7 2 Tubing Connections 7 7 Figure 7 3 Pump Tubing installation 7 8 Figure 7 4 Pump Assembly 7 9 Figure 7 5 Fuse Replacement 7 10 Figure 7 6 Sipper Pump Head Removal 7 11 Figure 7 7 Sipper Pump Seal Replacem...

Page 6: ...urbidity density viscosity pH volatility specific gravity temperature index of refraction optical activity or the presence of proteins or other biochemicals The Model 2700 SELECT can be set up to measure a number of different analytes but switching between them may entail some time and effort Configured as a single channel unit the Model 2700 SELECT provides quick measurements of any one of the fo...

Page 7: ... 232 interface YSI provides a full line of reagents to support the most common chemistries YSI offers the Model 2710 Turntable for batch sampling of up to 24 tubes or vials The 2710 Turntable is controlled by the 2700 SELECT software via a control cable provided The results of batch sampling are displayed and printed by the 2700 SELECT YSI offers the Model 2730 Monitoring and Control Accessory for...

Page 8: ...e results in about one minute Fluid sensing minimizes carry over Fluid design washes inside and outside of sipper tube Programmable automatic calibration Selectable concentration units including g L mg L mmol L w v Liquid crystal display and built in data printer RS 232 serial port Turntable interface circuitry installed User accessible service and diagnostic menus ...

Page 9: ...in 90 seconds May vary with analyte and sample matrix Power requirement 110 120 VAC or 220 240 VAC 50 60 Hz 50 Watts nominal Working environment Ambient temperature 15 to 35 Celsius Relative humidity 10 to 90 non condensing Instrument dimensions 10 0 x 14 0 x 14 0 inches 25 4 x 35 6 x 35 6 centimeters Instrument weight 25 pounds 11 4 kilograms ...

Page 10: ...ical Membrane Working Life 5 days Membrane O ring Color Green Potential Substrate Interference Methanol L Glutamate YSI Membrane 2754 Detection Range 0 10 mmol L Calibration Point 5 00 mmol L Linearity Check Point 10 0 mmol L Precision CV n 10 2 Linearity 0 to Cal Point 2 Linearity Cal to Range Max 5 Typical Membrane Working Life 7 days Membrane O ring Color Yellow User may configure instrument to...

Page 11: ...al Membrane Working Life 10 days Membrane O ring color Blue Choline YSI Membrane 2771 Detection Range 0 450 mg L Calibration Point 175 mg L Linearity Check Point 450 mg L Precision CV n 10 2 Linearity 0 to Cal Point 2 Linearity Cal to Range Max 5 Typical Membrane Working Life 7 days Membrane O ring color Orange L Glutamine YSI Membrane 2735 Detection Range 0 8 mmol L Calibration Point 5 00 mmol L ...

Page 12: ... standards for your application YSI also recommends that you confirm that no significant levels of interfering substrates are present in your samples For example we know that lactose and galactose in the same sample may interfere if significant concentrations of both exist Also dextrose will read at a sucrose membrane however by using the dual channel approach to dextrose and sucrose this is autom...

Page 13: ...that informed and safe operation is more than just a matter of knowing which buttons to push An understanding of the principles of operation and potential chemical interferences is necessary for the wisest interpretation of results Thorough precautions regarding the handling of biological samples are also essential for the safety of operators The early parts of this manual will teach you how to ge...

Page 14: ...ine power cord supplied with the instrument Connect the plug to a matching three pronged wall receptacle 3 Use ONLY fuses of the type supplied Section 7 9 Replacement power cords and fuses can be obtained from YSI or your Dealer Representative Appendix F G 4 Do NOT use an extension cord without protective grounding 5 Do NOT remove rear cover There are no user serviceable parts inside 6 Repairs are...

Page 15: ...h the following descriptions and familiarize yourself with the major components NOTE In maintenance kits service manuals and part lists the Sipper may be referred to as the Sipper Needle or Sipper Tube Figure 2 1 Inside Front View of the 2700 SELECT ...

Page 16: ...lt operations when the Buffer or Calibrator Bottles are empty or when the Waste bottle is full The Sample Chamber is made of clear acrylic plastic White and black holders for the sensor probes are screwed to either side The immobilized enzyme membranes on the sensor probes are mounted on O rings which act as fluid seals on each side of the Sample Chamber A reference or auxiliary electrode is house...

Page 17: ...ion It houses the fuses and pulls out of the case for fuse replacement The Power Receptacle is a power inlet One end of the power cord plugs into this receptacle while the other end plugs into an electrical outlet The Power Switch is an on off toggle switch 0 off and I on It is located on the back of the instrument ...

Page 18: ...he prepared buffer into the supply bottle replace the lid and reconnect the lead Install the calibrator solution One or more of the following YSI calibration standards will be provided in your starter supplies YSI 2776 Dextrose and L Lactate YSI 2780 Sucrose YSI 2783 Lactose YSI 2772 Choline YSI 2755 L Glutamate YSI 2736 L Glutamine YSI calibrators may be purchased separately Unplug the electrical...

Page 19: ...y configured as a Single Channel or a Dual Channel unit For the single channel configuration the white probe is a blank see Figure 2 4 Figure 2 4 Sample Chamber Sensor Configurations One or more packs of YSI Immobilized Enzyme Membranes is provided in the starter supplies Each pack contains four membranes Enzyme Membrane O rings are color coded for each type of chemistry For dual channel configura...

Page 20: ...e Using the plastic membrane holder press the O ring membrane assembly gently onto the probe face Figure 2 5 Wipe excess salt solution from the probe body then return the probe to the sample chamber Finger tighten the probe retainer so that the O ring seals the probe in place Do not overtighten Repeat this procedure for the second probe if you have a dual channel unit Return the membrane holder to...

Page 21: ... Main Menu Display Default Parameters Display The instrument will normally arrive with parameters including date and time set from the factory However if the backup rechargeable batteries have run down you will see the Default Parameters message This message will also appear if you ever need to change batteries in the future If you see the Default Parameter message press any key to access the Setu...

Page 22: ...itor 6 Turntable You are now ready to align the Sipper prime the fluid pumps and adjust the stirring Since the adjustments were made prior to shipment this procedure will likely be just a check IMPORTANT The front door must remain open to carry out this procedure 0 To make certain the Sipper Arm and attached Sipper are correctly aligned follow the instructions below Press 1 for Sipper The Sipper A...

Page 23: ...igure 2 7 Sipper Adjustment Position Press 1 for Test Sipper position The Sipper will descend into the Sample Chamber The Sipper should not contact the stainless steel cone If the Sipper position is still not exactly right press 1 and readjust it once more as described above IT IS VERY IMPORTANT THAT THE SIPPER BE ACCURATELY ADJUSTED Select 1 to restart check sipper cycle 0 Exit 1 Home sipper posi...

Page 24: ...appear The stir bar has two operating speeds normal speed at which the stir bar rotates smoothly in the chamber and accelerated speed at which the stir bar loses synchronization with the motor housed below and jumps This jumping action helps clear the Sample Chamber of air bubbles during a flush cycle Adjust until stir bar jumps 1 Increase speed 2 Decrease speed Adjust the speed until the stir bar...

Page 25: ...unMode 4 Report 5 PrntSetup 6 Default During normal operation you will seldom need to enter the Menu Mode but it is important to familiarize yourself with the menu locations of the various parameters over which you have control See Section 5 3 for complete details of menu options Press 5 for PrntSetup The data printer will print the instrument setup parameters This will take less than a minute and...

Page 26: ...by 3 AutoCal 4 Monitor 3 RunMode In this menu you select parameters related to sampling protocol set parameters that control automatic switching to standby mode and select parameters that will trigger auto calibrations In Sample Protocol you can select the sample station position activate replicate sampling and sample identification prompts set the position to which the Sipper descends when sampli...

Page 27: ...ll default parameters i e those set in the software to serve your needs for all standard applications Using this command also requires that you reset date time and all other general parameters Press ENTER to confirm No and return to Select Setup menu Now press MENU to return to Main Menu display Please select instrument mode RUN STANDBY MENU ...

Page 28: ...stituted and poured into the Buffer Bottle and YSI 2776 Standard 2 50 g L dextrose and 0 50 g L L lactate has been installed into the Calibrator Bottle position The instrument has been powered and the printer paper installed Let us also assume that you have your sample in a test tube and want to program the unit to run the sample three times in succession triplicate You would also like to identify...

Page 29: ...equired Date 14 Enter day Enter date and time as required Hour 17 Enter hour Enter date and time as required Minute 22 Enter minute Select date format MM DD YY 1 MM DD YY 2 DD MM YY Select and or confirm the format you desire for printed dates month day year day month year With the entry of the date format you will return to the previous menu level General setup General setup 1 Date Time 2 Contras...

Page 30: ...m your choice by pressing ENTER to return to the General setup General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 5 for Serial Enter instrument serial number 98 12345 The serial of your instrument is recorded on the serial plate lower rear of case You will find it helpful to record this number in memory It will be printed in the detailed report format and will be ver...

Page 31: ...e options range from 5 to 65 µL If you enter a number outside of this range the display will show you the limits Press ENTER to confirm 25 µL and return to the Measurement parameter setup menu level Measurement parameter setup 1 SampleSize 2 CalMethod 3 Black 4 White Press 2 for CalMethod Select calibration method One Station 1 One Station 2 Two Stations Press ENTER to confirm One Station since bo...

Page 32: ...tor label NOTE If you are using the default values and you choose a concentration unit other than g L the value automatically changes That is 0 50 g L becomes 5 62 mmol L or 500 mg L When non standard calibration values are used however the appropriate numbers must be entered whenever the measurement unit is changed ALWAYS VERIFY THAT THE CORRECT CALIBRATION VALUE IS ENTERED Use the information in...

Page 33: ... the 30 second endpoint parameter Measurement parameter setup 1 SampleSize 2 CalMethod 3 Black 4 White Press 0 to return to the Select setup level Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 3 for RunMode RUN mode setup 1 SampleProtocol 2 AutoStandby 3 AutoCal 4 Monitor Press 1 for SampleProtocol SamplingProtocol setup 1 SipperHeight 2 Replicates 3 ID 4 St...

Page 34: ...D Prompt sample ID No 1 No 2 Yes Again our example setup requests that we use sample identification Press 2 for Yes Prompt sample ID Yes 1 No 2 Yes Press ENTER to confirm and return to Sampling protocol setup SamplingProtocol setup 1 SipperHeight 2 Replicates 3 ID 4 Station 5 Turntable Press 4 for Station Enter Sample Station 2 The default station is Station 2 which is where our test tube will be ...

Page 35: ...utocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error Each parameter in this menu level can be used to alter the conditions which trigger autocalibrations For now simply explore each menu parameter Access each parameter by pressing the number Press ENTER to confirm the default setting and return to the Autocal setup See Section 5 3 for more information 0 6 7 Autocal setup 1 Tempera...

Page 36: ...of this report When finished the display again shows the Select setup menu Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 6 for Default Reset all system parameters No 1 No 2 Yes This menu allows you to reset all system parameters to the default settings that is those that would appear if you were to unpower the instrument including backup battery power Do not...

Page 37: ...nded system parameters for your particular chemistry application Next use this information to setup the 2700 SELECT as we have demonstrated above HINT Use the Print Setup option to confirm that you have correctly setup your instrument Remember PrntSetup can be accessed from Main Menu by pressing MENU then 2 for Setup and then 5 for PrntSetup Again refer to Figure 5 2 Menu Flow Chart for an overvie...

Page 38: ...ction 1 Service 2 Setup 3 Diagnostic Press 3 for Diagnostic Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 3 for Probe B LAC 4 23 nA W DEX 3 10 nA 1 Flush 2 Calibrator 3 Sample Observe the probe current values If they are above 6 nA nA nanoamp check to see if they are decreasing in value Check the Sample Chamber it should be full of buffer If necessary press 1 for Flush The Buffer P...

Page 39: ...ake a day or two and does not usually affect operation during the first day since autocalibrations compensate for probe current drift When enzyme membrane replacements are required in the future stabilization will occur much more rapidly usually within several minutes of installation if there has been no power disruption to the instrument ...

Page 40: ... the unit automatically transfers to STANDBY Mode where reagents are conserved In Section 5 you will learn how to change this 2 hour threshold value to better fit your application If you press STANDBY from Main Menu the instrument flushes buffer through the Sample Chamber that houses the Enzyme Electrodes It then continues to flush buffer once an hour to maintain fresh solution in the Sample Chamb...

Page 41: ...eans it will ready itself to calibrate Two or more calibration cycles will be run automatically The Sipper moves out of the Sample Chamber and enters the Calibrator Well or other preset calibrator station Calibration standard is aspirated into the Sipper which then returns to the Sample Chamber and dispenses the standard After the measurement the Buffer Pump flushes the standard from the chamber T...

Page 42: ... 3 2 accepts tubes from 9 to 16 millimeters in diameter and 50 to 100 millimeters long Any container other than a tube with the dimensions indicated should be presented at the Manual Station Station 3 Some double wall plastic tubes may prevent the fluid detection from working properly These tubes should also be presented at the Manual Station For the purposes of demonstrating basic operation choos...

Page 43: ...3 4 Figure 3 2 Sampling Stations Figure 3 3 The Test Tube Holder Pivoted Out ...

Page 44: ... falls outside these limits recalibrate the instrument by pressing CALIBRATE After recalibrating repeat the analysis as directed above If your reading is still outside this range proceed for now Later in this section specific actions for nonlinearity will be addressed Operation at the Manual Station 3 Remove the tube from the Test Tube Holder Rotate the Tube Holder back into place Next press 3 on ...

Page 45: ...esults are then displayed and printed as shown above Again compare the value reported by the instrument with the value on the label of the YSI 1531 Standard 9 00 g L dextrose Repeat calibration and sampling if the result is not within specified tolerance limits of 5 as described above If your reading is still outside this range refer to Linearity Test below Figure 3 4 The Manual Station ...

Page 46: ...in a sample ready status before reverting to STANDBY Mode Press ENTER then MENU to return to the Main Menu To ensure that your 2700 SELECT is operating properly perform the following operational checks on a daily basis It is advisable to maintain an instrument log book in which dates and lot numbers of reagents are recorded along with information from daily operational checks and other relevant in...

Page 47: ...inearity standard to test the linear range of the chemistry or chemistries you have chosen See the list below to be certain you are testing with the correct standard solution Place your instrument in RUN Mode When the unit is ready the following display will appear Ready to sample at Station 2 Pour a small amount of linearity standard into a test tube or cup and run it as a sample at either the Ma...

Page 48: ...see Section 4 for more information If you use units other than g L for the linearity test refer to Appendix A for conversion values NOTE If any reading is outside of the specified tolerance limits recalibrate and repeat the linearity test If the reading is still out of tolerance refer to Section 8 Troubleshooting ...

Page 49: ...3 10 ...

Page 50: ...with the sample after the analysis is made YSI s technology allows preservation of the enzyme by immobilizing it in a patented membrane structure The enzyme membrane is coupled with an electrochemical probe and housed in a sample chamber where sample delivery and flushing occur The life of the enzyme membrane is measured in days or weeks and is not dependent on the number of samples analyzed If us...

Page 51: ...ducing compounds can interfere Typical interferences include phenols hydrazine hydrogen sulfide and mercaptans A variety of sample types can be analyzed with the 2700 SELECT Generally the only sample preparation that may be required is dilution of the sample to bring the substrate concentration within the linear range of the instrument see Section 1 4 for the working range of each chemistry Neithe...

Page 52: ... unit set for simultaneous dextrose and L lactate analyses The parameters you see in these printouts are default values For many applications the default settings will be appropriate for you Now enter the Measurement Parameter menu press 2 for MeasParameter Within this menu level you will confirm or change the parameter settings that define the chemistry or chemistries of interest Sample Size 1 Sa...

Page 53: ...1 In this setup the instrument is programmed to measure the dextrose reading at the sucrose membrane and automatically subtract it Black Probe Parameter 3 Black In this menu you choose parameters that correspond to the chemistry of interest at the Black Probe Be certain that you install the correct enzyme membrane type The selections include Chemistry 10 selections plus none Unit of Concentration ...

Page 54: ...ity performance to 25 0 g L YSI 2777 25 0 g L dextrose may be used as a linearity standard provided the sample size is 10 µL If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Special Considerations If sample dilution is required use reagent water or 2357 buffer If a solution must be prepared from solid dextrose use the followi...

Page 55: ...Cal Well Black Probe Parameters Chemistry L Lactate Unit of Conc g L Cal value 0 50 End Point 30 sec If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations If sample dilution is required use reagent water or 2357 buffer D Lactate is not a substr...

Page 56: ...Black Probe YSI 2703 Sucrose Membrane White Probe n a Sample Size 25 µL Cal Station Station 1 Cal Well Black Probe Parameters Chemistry Sucrose Unit of Conc g L Cal value 5 00 End Point 30 sec If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Consideratio...

Page 57: ...nc g L Cal value 5 00 End Point 30 sec If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations If sample dilution is required use reagent water The sample should be galactose free Galactose and other galactosides such as raffinose and stachyose a...

Page 58: ...The linear range of ethanol is quite limited If you are concerned about linearity monitor the upper range of concentration on a regular basis You may benefit by preparing and using a calibrator with an ethanol concentration close to your sample concentration If you prepare your own ethanol calibrator prepare a solution with ionic strength Level sensing at Station 2 Test Tube Holder depends on a co...

Page 59: ...as a linearity standard provided the sample size is 10 µL If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Special Considerations An amyloglucosidase enzyme such as Sigma A7420 works with many samples Solubilizing starch is key to obtaining reproducible results A variety of methods are in the literature YSI has documented a c...

Page 60: ...ress the sample as a salt of choline you must enter an adjusted calibration value when configuring your instrument measurement parameters For example to express results as a choline hydroxide value program the calibrator value as 204 mg L when using the YSI 2772 Choline Standard calibrator solution Your results will now reflect the concentration of choline hydroxide in the sample Assumptions and c...

Page 61: ... 30 sec YSI does not currently offer galactose standard solutions If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations If sample dilution is required use reagent water The sample should be lactose free Lactose and other galactosides such as ra...

Page 62: ...chemistry Note See Appendix A if concentration unit conversion is required Special Considerations Since hydrogen peroxide is typically used to calibrate the YSI Blank Membrane the enzyme catalase is a concern Catalase destroys hydrogen peroxide Catalase occurs naturally and may be in the sample you are testing If so it can also accumulate in the 2700 SELECT sample chamber A small amount of sodium ...

Page 63: ...d use reagent water or 2357 Buffer If you want to measure MSG monosodium glutamate monohydrate and express the results as mmol L of MSG the calibration parameters listed above are all appropriate If you want to express results as a w v measurement such as mg L MSG then correction for the sodium and water components is necessary To express results as an MSG value change the glutamate units to a w v...

Page 64: ...ne Membrane White Probe n a Sample Size 20 µL Cal Station Station 2 Black Probe Parameters Chemistry Glutamine Unit of Conc mmol L Cal value 5 00 End Point 30 sec If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations If sample dilution is requi...

Page 65: ...e linear range of methanol is quite limited If you are concerned about linearity monitor the upper range of concentration on a regular basis You may benefit by preparing and using a calibrator with a methanol concentration close to your sample concentration If you prepare your own methanol calibrator prepare a solution with ionic strength Level sensing at Station 2 Test Tube Holder depends on a co...

Page 66: ...p this simultaneous measurement was described System Buffer YSI 2357 Calibrator Std YSI 2776 2 50 g L dex 0 50 g L L lact Linearity Std YSI 1531 9 00 g L dex and YSI 1530 2 67 g L L lact Black Probe YSI 2329 L Lactate Membrane White Probe YSI 2365 Dextrose Membrane Sample Size 25 µL Cal Station Station 1 Cal Well Black Probe Parameters Chemistry L Lactate Unit of Conc g L Cal value 0 50 End Point ...

Page 67: ...SI 2703 White Membrane YSI 2365 Sample Size 25 µL Cal Station Two Station 1 dex 2 suc Black Probe Parameters White Probe Parameters Chemistry Sucrose Chemistry Dextrose Unit of Conc g L Unit of Conc g L Cal value 5 00 Cal value 2 50 End Point 30 sec End Point 30 sec Since sucrose calibrator is at the Test Tube Holder you must sample at the Manual Station 3 The Turntable position 4 and the Monitor ...

Page 68: ...nal may result in significant errors in the sucrose reading An alternative method involves using two different and appropriate dilutions of the sample to first measure free dextrose then treat one sample with invertase and analyze for dextrose liberated from the sucrose Contact YSI Customer Service or your dealer representative for an application note explaining this approach in detail ...

Page 69: ...ived from Glutamine reaction System Buffer YSI 2357 Calibrator Std YSI 2755 and YSI 2736 Linearity Std YSI 2756 and YSI 2737 Black Membrane YSI 2735 White Membrane YSI 2754 Sample Size 20 µL Cal Station Two Station 1 glutamate 2 glutamine Black Probe Parameters White Probe Parameters Chemistry Glutamine Chemistry Glutamate Unit of Conc mmol L Unit of Conc mmol L Cal value 5 00 Cal value 5 00 End P...

Page 70: ... the unit will automatically update its calibration as required In MENU Mode there are three submenu levels Service Setup and Diagnostics Notice that you may move from RUN Mode or STANDBY Mode to limited menus in Setup However to fully access MENU Mode you must exit RUN or STANDBY modes Figure 5 1 2700 SELECT Software Structure To exit RUN Mode press RUN then confirm your exit by pressing 2 for Ye...

Page 71: ... menus by selecting options on the display You can press MENU to get back to the Main Menu and 0 to back up to the previous display However it may be necessary to confirm a response by pressing ENTER before continuing to use the MENU or 0 keys Please select instrument mode RUN STANDBY MENU ...

Page 72: ... for Sipper to initiate Sipper alignment This procedure was used during initial instrument setup Refer to Section 2 9 for more information The Sipper Arm Assembly will move to the home position Loosen the adjustment screw that holds the Sipper flange to the arm to center the Sipper over the Sample Chamber injection port The display messages will guide you through the procedure It is very important...

Page 73: ...d jumps This jumping action helps clear the sample chamber of air bubbles during a flush cycle Adjust until stir bar jumps 1 Increase speed 2 Decrease speed Using the 1 Increase and 2 Decrease choices adjust the Stir Bar Lower the speed to observe synchronous rotation then increase the speed until the stir bar jumps and looses synchronous rotation At this point release the 1 key If jumping does no...

Page 74: ...ility you have in setting up your 2700 SELECT You may find it helpful to use the Menu Flow Chart Figure 5 2 to follow the menu levels Main Menu display Please select instrument mode RUN STANDBY MENU From Main Menu press MENU to display instrument functions Select instrument function 1 Service 2 Setup 3 Diagnostic Press 2 for Setup to display setup selection categories Select setup 1 General 2 Meas...

Page 75: ...hen finished press 0 to return to General setup level Adjust the display contrast 1 Raise contrast 2 Lower contrast General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 3 for RS232 Use this menu to set communication parameters if you are using the serial interface port See Section 9 for detailed information on communications Below are the display messages showing defau...

Page 76: ...5 Handshake 6 Configuration If you press 6 for Configuration you will see the following Select configuration Non multidrop 1 Non multidrop 2 Multidrop The configuration option allows operation in either a point to point environment or in a multidrop environment with an address ID assigned to your 2700 SELECT unit s The default configuration is Non multidrop If you press 2 for Multidrop you will se...

Page 77: ... character and omit the last two Example 98B01234AB can be entered as 98 01234 This number is normally set prior to delivery and preserved in memory by battery backup Press ENTER to confirm and return to General setup level Enter instrument serial number 98 01234 General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 6 for Level Sensor Choose Yes to maintain level sensin...

Page 78: ...tup 1 SampleSize 2 CalMethod 3 Black 4 White Press 2 for CalMethod The default setting is One station The Two Station option is required for some dual channel analyses for example simultaneous dextrose and sucrose determination Select calibration method One station 1 One station 2 Two stations After pressing ENTER to confirm One station you must specify at which location the calibration solution w...

Page 79: ...ntries you may need to lead with a 0 Follow the cursor For example if you had 13 89 mmol L Dextrose entered and you wanted to change to 1 80 g L the calibration value would read 02 50 with the cursor under the 0 You would then need to enter 1 80 as 01 80 To confirm press ENTER and then choose the end point time for the stabilized enzyme sensor reading The default is 30 seconds The range is 15 to 1...

Page 80: ...his specifies to what vertical position the Sipper descends when sampling at the Manual Station 3 For example with long test tubes and the fluid level near the bottom of the test tube use Low setting Select manual sipper height Medium 1 Low 2 Medium 3 High Press ENTER to confirm and return to Sampling protocol setup Sampling protocol setup 1 SipperHeight 2 Replicates 3 ID 4 Station 5 Turntable Pre...

Page 81: ...r Station to assign the position at which the sample will be aspirated The default location is Station 2 the Test Tube Holder station You may also elect to sample from Station 3 the Manual Station or even Station 1 the Cal well This last choice would be rarely used but does give you the flexibility to calibrate from a solution in the Test Tube Holder 2 and sample from the Cal Well 1 If you have th...

Page 82: ...er compared to the last cal and 5 after certain errors are detected eg baseline instability Autocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error You may alter any of these parameters to suit your application however you may compromise precision and or accuracy when doing so YSI s stated specifications are based on the default settings shown above 0 1 2 3 These selections are prov...

Page 83: ...ples from 0 to 30000 Autocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error Press 4 for Cal shift Enter allowable autocal shift in 0 to disable 2 Confirm value shown or enter and confirm any number from 0 to 100 Autocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error Press 5 for Sample error Autocal on sample error Yes 1 No 2 Yes Press ENTER to confirm performing an ...

Page 84: ...rature changes with newly installed reagents or enzyme membranes You may also elect to choose Detail report for the first few calibrations or samples after a long idle period where no samples or cals are run If you suspect problems with the instrument you should run Detail reports to provide more information for troubleshooting See Appendix D for example report formats Press 0 to return to Select ...

Page 85: ...mmended settings if you have been experimenting with the selections and would like to start at the beginning Press ENTER to confirm your choice and return to Select setup Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 0 to return to Select instrument function Select instrument function 1 Service 2 Setup 3 Diagnostic YOU HAVE NOW COMPLETED THE SETUP MENU OF TH...

Page 86: ...sensors in the system These include the temperature sensor in the Sample Chamber the level sensors in the reagent and waste bottles the Sipper capacitance sensing and the optical sensors that control motors the Sipper Pump and sense the Test Tube Holder position Follow the display prompts and information below to learn about the 2700 SELECT menu diagnostics mode As before you will often need to pr...

Page 87: ...c 1 Sipper motor 2 Stir motor 3 Turntable Press 1 for Sipper motor to display the following motion selections Sipper diagnostic 1 Up 2 Down 3 Home 4 Clockwise 5 Counterclock 6 Exercise When you press one of the keys 1 5 a movement of the Sipper should be observed Press 1 and the Sipper moves up until the upper limit optosensor is detected Press 2 and the Sipper moves down into the Sample Chamber o...

Page 88: ...n The asynchronous motion is used to dispel air bubbles from the chamber wall and probe surfaces You turn the Stir Motor off by pressing 2 again The Stir motor may be activated with the Sipper either in or out of the Sample Chamber Motor diagnostic 1 Sipper motor 2 Stir motor 3 Turntable Press 0 to return to the five diagnostic selections Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Pre...

Page 89: ...splay updates to read the following Sipper pump diagnostic 1 Home 2 Dispense separator 3 Exercise Press 2 for Dispense separator A short extension of the plunger should occur This simulates dispensing the air bubble into the Sample Chamber after the sample reading at the probe would have occurred The plunger is now back to home position Sipper pump diagnostic 1 Home 2 Aspirate separator 3 Exercise...

Page 90: ...f external pump operation Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 3 for Probe You will observe a brief display message as shown below which then changes to show probe current s You will observe only Black probe current for a single channel unit The probe activity is expressed in nA nanoamperes of electrical current A nanoampere is 10 9 amperes which is a very low level of ele...

Page 91: ...oncentrations used for sampling B LAC 91 83 nA W DEX 112 9 nA 1 Flush 2 Calibrator 3 Sample Press 0 to return to the five diagnostic selections Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 4 for I O From this menu level you can run diagnostic tests on the input output devices of the instrument I O diagnostic 1 Keypad 2 Display 3 Printer Press 1 for Keypad You may now test each of ...

Page 92: ...ppear in each of the 40 segments of the liquid crystal display The total test takes about 40 seconds Terminate the test at any time by pressing any key The first few characters are shown below I O diagnostic 1 Keypad 2 Display 3 Printer Press 3 for Printer The printer prints the same 95 characters described above in the display test The printout uses 5 lines and takes about 10 seconds to complete ...

Page 93: ...evelSensor 3 OptoSensor Press 2 for LevelSensor You will observe the current status of each of the four level sensors shown below The Sipper will show fluid or air The Cal and Buffer will show OK or Empty The Waste will show OK or Full Level sensor status Sipper Fluid Cal OK Waste OK Buffer Empty Press 0 to return to Sensor diagnostic menu Sensor diagnostic 1 Temperature 2 LevelSensor 3 OptoSensor...

Page 94: ...Press 0 to return to the five diagnostic selections Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 0 to return to Select instrument function Select instrument function 1 Service 2 Setup 3 Diagnostic Press 0 to return to Main Menu display Please select instrument mode RUN STANDBY MENU NOTE You may press MENU from most levels of diagnostics to return directly to Main Menu You now have...

Page 95: ...5 26 ...

Page 96: ...5 in the YSI 23A Blood Glucose Analyzer then later in the YSI 27 Industrial Analyzer and the YSI 23L Blood Lactate Analyzer Most recently the same technology has been employed in the YSI 2300 STAT PLUS Glucose and L Lactate Analyzer and the YSI 1500 SPORT Lactate Analyzer Figure 6 1 Sensor Probe and Enzyme Membrane Each probe is fitted with a three layer membrane containing immobilized enzyme in t...

Page 97: ... to the steady state H2O2 concentration and therefore to the concentration of the substrate REACTION 1 glucose β D glucose O2 GOx Glucono δ lactone H2O2 REACTION 2 H2O2 Pt anode 2H O2 2e The platinum electrode is held at an anodic potential and is capable of oxidizing many substances other than H2O2 To prevent these reducing agents from contributing to sensor current the membrane contains an inner...

Page 98: ...6 3 The 2700 SELECT fluid and electromechanics system is schematically described below in Figure 6 2 Figure 6 2 2700 SELECT Fluid System ...

Page 99: ...5 nA nanoamps for YSI calibration solutions The maximum net probe current is about 500 nanoamps The 2700 SELECT monitors the probe baseline current for nA activity and stability If an unstable baseline is detected the buffer pump will continue to flush the sample chamber with buffer When a stable baseline is established an automatic calibration is initiated After every calibration and sample the f...

Page 100: ...default setting between the present and previous net calibration currents occurs the instrument repeats calibration The sensors net current for a calibration is displayed and printed An unstable calibration is displayed and printed as a cal shift While establishing a stable calibration the 2700 SELECT will run 5 calibrations before the error unable to calibrate is displayed The flexible parameter ...

Page 101: ...calibrator waste and supply bottles The Sipper level sensor detects the sample surface at the Test Tube Sample station and then travels into the sample about 3 millimeters 1 8 inch This controlled immersion depth permits the use of sample tubes that are filled to different heights without significant carry over between samples This same technique can be employed when using the optional 2710 Turnta...

Page 102: ...and you may enter STANDBY Mode by pressing STANDBY You may switch back and forth from RUN Mode to STANDBY Mode by using these keys and confirming your choice by pressing ENTER To return to the Main Menu to have access to the full Menu options of Service Setup and Diagnostics you must exit RUN Mode or STANDBY Mode by pressing RUN to exit RUN Mode or pressing STANDBY to exit STANDBY Mode You have li...

Page 103: ...6 8 ...

Page 104: ... 5 Probe Cleaning is not recommended unless problems related to the enzyme sensor system are indicated Empty the Waste Bottle If the 2700 SELECT is used for medical research or biological testing dispose of the waste bottle contents in a manner suitable for biohazardous waste The reagents used in the 2700 SELECT are non toxic and unless otherwise specified consist of a phosphate salt buffer with s...

Page 105: ...sh the buffer bottle Prepare it in a clean bottle with cap You should store it at room temperature After a buffer change prime the buffer system From Main Menu press MENU See menu information displayed above Next press 1 for Service then press 2 for Buffer to initiate a 15 second run of the Buffer pump Make certain that all air is out of the buffer tubing Buffer should be exiting the Sipper inside...

Page 106: ...ominal 1 solution of hypochlorite 1 part commercial bleach with 3 parts deionized or distilled water and place this solution in a clean 250 mL bottle that has threads compatible with the Cal Cap Assembly at Station 1 Attach the Cal Cap Assembly tighten and shake gently to insure thorough washing of the inside of the cap assembly From the Service Menu initiate cal priming to flush hypochlorite solu...

Page 107: ...ntable Sipper diagnostic 1 Up 2 Down 3 Home 4 Clockwise 5 Counterclock 6 Exercise Unscrew and remove all three probes from the sample chamber If your membranes are in good condition that is if they pass the tests in Section 3 3 it is not necessary to replace them It is important that the membranes do not dry out If the chamber is to be disassembled from the instrument for more than 15 minutes the ...

Page 108: ...calibrator well tube and drain tube Refer to Section 2 6 to check the Sipper alignment and prime the buffer and calibration systems Figure 7 1 Sample Chamber Illustration To assure proper performance and prevent unexpected down time check membrane integrity and linearity on a daily basis Refer to Section 3 3 Daily Operational Checks for details When enzyme membrane installation is required refer t...

Page 109: ...or in a 14 solution of ammonia for 3 minutes 2 Immediately after soaking rinse the sensor with reagent grade water for 3 5 minutes It is important that all the residual ammonia is removed 3 Prepare a small amount of 0 5 sodium hypochlorite solution 4 Immerse the sensor in the solution for 30 45 seconds Check periodically sensor should darken but do not allow it to become black 5 Remove the sensor ...

Page 110: ...g the buffer and calibrator pumps to the instrument wall and remove the pumps Remove the front housing from each pump by pulling it out at the bottom Remove the roller assembly and tubing from each pump Disconnect and remove all the instrument tubing New tubing is supplied in the Preventive Maintenance Kit Connect the new tubing as shown in Figure 7 2 When installing new pump tubing insert the rol...

Page 111: ...hat tab on the rear pump housing into the slot at the top of the front pump housing see Figure 7 4 Lower the front pump housing while guiding the tubing into the slots at the bottom Gently squeeze the two housings together while pushing up on the roller assembly Align the slot in the rear of the pump with the motor shaft then slide the pump onto the shaft Remount the pump to the instrument wall us...

Page 112: ...e Sipper ground cable and tubing to the new Sipper Refer to Section 2 6 to readjust the Sipper The Sipper Ground Cable flexes with every sample After several thousand samples the cable may break To prevent unexpected down time replace this cable every 6 months Extra cables are supplied in the Preventive Maintenance Kit Pull the connector out of the printed circuit board on the Sipper Arm Remove th...

Page 113: ...Fuse Type 110 120 VAC Operation 1 Amp YSI 062443 Fast acting 3AG 250 volt 0 25D x 1 25L inches For Europe and UK use fuse type 220 240 VAC Operation 1 2 Amp YSI 019722 Anti surge T 250 volt 5mm x 20mm L IEC127 BS4265 Install new fuses in place of the old ones and return the fuse holder to the power receptacle Be certain the correct voltage is selected The arrowhead on the power selector must be po...

Page 114: ...er Pump Remove the Sipper Pump head from the instrument wall see Figure 7 5 Figure 7 6 Sipper Pump Head Removal Pull the white pump base from the clear pump housing If salt deposits have formed on the parts wash them off with warm water Replace the seals as shown in Figure 7 6 Extra seals are supplied in the Preventive Maintenance Kit ...

Page 115: ...7 12 Figure 7 7 Sipper Pump Seal Replacement Reassemble the pump position the plunger as shown in figure 7 7 and install it back on the instrument Figure 7 8 Sipper Pump Plunger Position 0 1 2 3 4 5 6 7 ...

Page 116: ...uild up or if the mechanism squeaks or jams then clean the screw and guide rods with a lint free towel Apply Lubriplate 105 grease supplied in YSI 2788 Preventive Maintenance Kit to two or three threads of the Acme screw Normal operation will distribute the grease along the length of the screw Lubricate each guide rod with a drop of light machine oil Wipe off any excess lubricant promptly Figure 7...

Page 117: ...7 14 ...

Page 118: ...ommended time Look and listen for problems fluid leaks salt build ups air bubbles in the chamber loose connections noisy components etc Review Section 5 4 Menu Diagnostics to learn more about how you can test individual components of the 2700 SELECT Use the troubleshooting chart in this section to assist you in identifying the problem then use the chart to guide you to a corrective action If the p...

Page 119: ...ple printouts and explanations of the Detail format information Sample Report Detail Calibration Report Detail Sample Report CALIBRATION REPORT ID 123456789 05 03 B Lactate Unstable B L Lactate 4 82 g L IB current 1 79 nA IB current 2 11 nA PL current 11 63 nA PL current 118 65 nA FB current 1 51 nA Slope 4 69 nA min Base shift 2 65 End Point 30 Sec Slope 1 42 nA min W Dextrose 8 3 g L End Point 3...

Page 120: ...ated plateau slope An excessive slope can be an indicator of the presence of an interfering substance End Point is the time from dispensing the sample into the sample chamber until the instrument reads the probe signal The default value for most chemistry setups is 30 seconds The value that you have selected in Setup is displayed in the report Note This is not through put time but rather best thou...

Page 121: ...wly installed probe ACTION Enter probe diagnostics and check probe currents SECTION 2 6 POSSIBLE CAUSE Power disruption ACTION Enter probe diagnostics and check probe currents SECTION 2 6 POSSIBLE CAUSE Failing enzyme membrane ACTION Perform daily operational checks and replace membrane s if necessary SECTION 3 4 POSSIBLE CAUSE Enzyme Probe surface s fouled ACTION Clean probe surface s SECTION 7 5...

Page 122: ...her conductive body ACTION Reroute cables SECTION 2 3 SYMPTOM ERROR Motor Failure POSSIBLE CAUSE One of the motors is jammed ACTION Enter motor diagnostics and cycle the suspected motor SECTION 5 4 POSSIBLE CAUSE Dirty drive screw on sipper mechanism ACTION Clean and lightly oil SECTION 7 11 POSSIBLE CAUSE Worn sipper pump seals ACTION Replace seals SECTION 7 10 SYMPTOM ERROR Overrange POSSIBLE CA...

Page 123: ...Reposition and repeat sample SECTION SYMPTOM ERROR Unable to Calibrate POSSIBLE CAUSE Pinched leaking or disconnected tube ACTION Fix or replace tubing SECTION 7 6 POSSIBLE CAUSE Sipper misaligned ACTION Check Sipper alignment SECTION 2 6 POSSIBLE CAUSE Stir bar not in chamber ACTION Disassemble chamber and reinstall stir bar SECTION 7 3 POSSIBLE CAUSE Newly installed enzyme membrane ACTION Enter ...

Page 124: ... 2 3 POSSIBLE CAUSE Pinched blocked leaking or disconnected tube ACTION Fix or install new tubing SECTION 7 6 POSSIBLE CAUSE Calibrator pump not operating properly ACTION Replace tubing SECTION 7 6 POSSIBLE CAUSE Fluid not conductive ACTION Use saline as diluent SECTION 4 8 POSSIBLE CAUSE Low sample level ACTION Run sample at manual position SECTION SYMPTOM Blank Printout POSSIBLE CAUSE Printing o...

Page 125: ...ement parameters Remake standards SECTION 4 3 SYMPTOM Keypad Disabled POSSIBLE CAUSE Instrument in Remote Control Mode ACTION Switch communications mode or reset SECTION 9 3 POSSIBLE CAUSE Faulty keypad or cable ACTION Check keypad in I O diagnostics SECTION 5 4 SYMPTOM Printer Does Not Advance POSSIBLE CAUSE Paper or roll jammed ACTION Open paper well and clear obstruction Reset instrument if nec...

Page 126: ...00s and or other controllers to a host while non multidrop point to point refers to a single 2700 connected to a host controller The non multidrop configurations of software versions higher than 2 40 maintain compatibility with the earlier software version 2 03 The command format and communication protocols are identical for software versions 2 03 and up In sections below you will learn about comm...

Page 127: ...y to receive data and receives only when DCD is asserted DSR is not used Software Handshaking Signals In the XON XOFF handshaking protocol RTS and DTR are always asserted and CTS and DCD are always ignored Since the 2700 acts as a DTE it requires a shielded NULL modem cable to connect to another DTE such as the PC Figure 9 2 and Figure 9 3 show possible NULL modem cable connections Figure 9 2 Full...

Page 128: ...e display of an error code or for an illegal command A command is defined to be a string of printable upper case ASCII characters Blanks are ignored Command Grammar The command grammar is shown below Abbreviations include ESC escape cmd command addr address arg argument and cr carriage return ESC cmd arg cr non multidrop ESC addr cmd arg cr multidrop In this grammar addr is a single byte binary no...

Page 129: ...ode 2 2700 is busy in RUN mode Code 6 assignment of station is out of range s 1 or s 5 Code 7 purge time of zero is assigned when Station 5 s 5 is assigned monitor accessory Code 8 zero turntable position number is assigned t n 0 or default t n 0 Code 9 a sample or calibration results were not found when requested b 2700 is not in RUN mode c 2700 is in halt condition A halt condition can be caused...

Page 130: ...get the result records The host requests the results by either sending the sample ID number and getting the result record or by querying the unit for any untransmitted result in the data base Remote Control Mode Remote Control Mode is designed primarily for industrial and research applications where process control may be an important consideration In Remote Control mode the instrument is in RUN M...

Page 131: ...e information from that reading continues on the next line If two lines of information are combined the first is black probe data the second is white probe data In single channel units this will not be a concern In Figure 9 4 several examples show how information for a dual channel 2700 SELECT may appear when transmitted Again the ruler line is for reference only and does not appear on the record ...

Page 132: ... 56 123456789 H2O2 12345 78 mmol L 0000 cr lf 15 12 04 02 13 98 23 56 123 1 H2O2 45 78 nA 0000 cr lf 15 12 04 02 13 98 23 56 123 1 H2O2 15 28 nA 0F01 cr lf 12 02 34 02 13 98 24 86 2 H2O2 12345 78 mmol L 0000 cr lf 12 02 34 02 13 98 24 86 2 H2O2 345 78 g L 0000 cr lf Figure 9 4 2700 SELECT Data Report Format NOTE The specific information above is not intended to appear realistic but rather to accou...

Page 133: ...56789 123456789 123456789 123456789 12345 11 11 11 22 22 22 33333 444 555555555 6666 77777777 88888888 9999 cr lf 11 11 11 22 22 22 33333 444 555555555 6666 77777777 88888888 9999 cr lf FIELD OFFSET WIDTH DESCRIPTIONS NO COLUMN BYTE 01 01 08 Time 02 10 08 Date 03 19 05 Temperature 04 25 03 Node Address 05 29 09 Sample ID 0 no ID 1 Cal Report 2 Monitor Sample Report 3 Information Report 06 39 04 Pr...

Page 134: ...lt RS Report sample result with sample ID number RY Report status of instrument RX Report last transmission DATABASE MANAGEMENT RZ Clear database memory CONTROL TR0 Turn remote control off TR1 Turn remote control on TP0 Turn 2700 printer off TP1 Turn 2700 printer on MPt Change pump purge time MTt Change monitor time interval MRt Change precal time interval MOt Change postcal time interval MSs Chan...

Page 135: ...ent results Calibrate status result U Last cal not sent to host N No unsent calibration results Machine status I Idle in Run Mode S Processing sample C Processing calibration A Processing autocalibration M Processing manual sample P Processing precal cycle N Processing monitor cycle T Processing postcal cycle F Flushing and aborting error cycle B Stabilizing baseline current K Stabilizing calibrat...

Page 136: ...o 2700 RC Report last calibration result 2700 to Host cal result see format in Figure 9 4 When Report Sample Result RS is invoked the 2700 sends back the last sample processed When combined with an ID number the 2700 searches its database and sends back the most recent matching ID result After the result has been transmitted its entry in the database is marked as sent Another request for the same ...

Page 137: ...y you may enter Run Mode locally by pressing RUN and waiting for successful calibration Then invoke the TR1 command to enter remote control mode In a similar manner you can invoke the Turn Remote Control Off TR0 command to return to Result Reporting Mode In the Remote Control Mode you may elect to invoke the Turn 2700 Printer Off TP0 command The 2700 responses are outlined below Host to 2700 TP0 T...

Page 138: ...ting to enter Run Mode from a halt condition The calibration commands and sample process commands are outlined below The process sample commands are described together the only difference among the different commands being the station from which the sample is aspirated 1 2 4 5 Station 3 is theoretically possible but not practical in remote control mode When you invoke the Process Calibration PC co...

Page 139: ...n In these arguments s is the station number from which to sample If s is not given or NULL the currently assigned sample station is used The character t is the turntable starting position which is only valid when s 4 Remember station 4 is the turntable sampling position If t is not provided or NULL the default starting position will be used The character n is the number of turntable positions to ...

Page 140: ...23456789 123456789 123456789 12345 AA AA AA BB BB BB CCCCC DDD EEEEEEEEE FFFF GGGGG GG HHHHHHHH II J cr lf AA AA AA BB BB BB CCCCC DDD EEEEEEEEE KKKK LLLLL LL MMMMMMMM NN O cr lf In the fields above the characters represent the following A current system time hh mm ss 24 hour clock B current system date mm dd yy or dd mm yy depends on format C not used Always D comm node address if multidrop Blank...

Page 141: ... a radix 0 for 1 for b date format 0 for mm dd yy 1 for dd mm yy c bottle level sensor flag 0 for off 1 for on d sample size 5 to 65µL e sample station 1 to 5 f calibration method 0 for single 1 for dual Autocal g autocal on chemistry error flag 0 for off 1 for on h temperature shift between autocals i time interval between autocals 0 to 65535 minutes j number of samples between autocals 0 to 3000...

Page 142: ... where t is in seconds MTt change monitor time interval to t where t is in minutes MRt change precal time interval to t where t is in minutes MOt change postcal time interval to t where t is in minutes MSs change monitor station number to s Abort Turntable Sample Command PA You may use this command to abort turntable samples in progress Recall you initiate the turntable sampling using the PS comma...

Page 143: ...9 18 ...

Page 144: ...0 43 48 46 Galactose 2 50 2500 0 25 13 89 180 L Glutamate 0 73 731 0 07 5 00 146 L Glutamine 0 73 731 0 07 5 00 146 L Lactate 0 50 500 0 05 5 62 89 Lactose 5 00 5000 0 50 14 62 342 Methanol 1 00 1000 0 10 31 25 32 Peroxide 0 60 600 0 06 17 65 34 Sucrose 5 00 5000 0 50 14 62 342 Denotes that YSI does not currently offer this standard value If you are preparing your own standard of a value not liste...

Page 145: ...conversion s 2 Cancel units common in numerator and denominator 3 Multiply numbers mg L 2 50 g L 2 50 g L 1000 mg g 2 50 1000 mg L 2500 mg L w v 2 50 g L 2 50 g L 0 1 L dL 2 50 0 1 g dL 0 250 g dL 0 250 Note g dL is g 100ml or percent mmol L 2 50 g L 2 50 g L 1 mole 180 g 1000 mmole mole 2 50 1 180 1000 mmole L 13 89 mmol L ...

Page 146: ...39 1 389 0 139 10 5 1 23 1 228 0 123 8 40 L Glutamine 1 17 1 169 0 117 8 00 146 1 11 1 111 0 111 7 60 2 80 2 806 0 281 31 5 L Lactate 2 67 2 672 0 267 30 0 89 2 54 2 539 0 254 28 5 26 25 26 250 2 63 76 8 Lactose 25 00 25 000 2 50 73 1 342 23 75 23 750 2 38 69 4 2 63 2625 0 263 82 0 Methanol 2 50 2500 0 250 78 1 32 2 38 2375 0 238 74 2 26 25 26 250 2 63 76 8 Sucrose 25 00 25 000 2 50 73 1 342 23 75...

Page 147: ...mine 0 06 58 0 01 0 40 146 L Lactate 0 03 30 0 01 0 34 89 Lactose 342 Methanol 0 05 50 0 01 1 56 32 Sucrose 0 10 100 0 01 0 29 342 NOTE Use the values from the preceding tables only when calibrating with the appropriate YSI calibrator solution Choline 2772 Dextrose 2776 Ethanol 2790 L Glutamate 2755 L Glutamine 2736 L Lactate 2776 Methanol 2726 and Sucrose 2780 See Section 3 Basic Operation Daily ...

Page 148: ...tion Quantity 2726 Methanol Standard Kit 1 00 g L 180mL 2 50 g L 120mL 2736 L Glutamine Standard 5 00 mmol L 250mL 2737 L Glutamine Standard 8 00 mmol L 156mL 2755 L Glutamate Standard 5 00 mmol L 250mL 2756 L Glutamate Standard 10 0 mmol L 125mL 2772 Choline Standard 175 mg L 250mL 2773 Choline Standard 450 mg L 125mL 2776 Dextrose L Lactate Standard Dex 2 50 g L Lac 0 50 g L 250mL 1530 L Lactate...

Page 149: ...embrane 1liter 2705 Lactose Buffer Kit use with 2702 membrane 4liters 2787 Ethanol Buffer Kit use with 2786 membrane 4liters 2363 Potassium Ferrocyanide 1000 mg dL 125mL 2392 NaCl Solution sodium chloride 30mL Reagent packaged dry Makes specified volume when reconstituted Reagent concentrated Makes specified volume when reconstituted Miscellaneous Accessories Supplies YSI Product Description 2710 ...

Page 150: ...nt Setup prints out on the paper tape This takes about 60 seconds The information on the printout completely describes the system parameters whether using default settings or settings that you configured for your particular application You can learn about the system parameters by referring to Section 5 3 Setup in this manual If you have a YSI 2710 Turntable refer to Section 3 in the 2710 Turntable...

Page 151: ... TT FluidDet ON Replicates OFF MONITOR Sample ID OFF MonStation 1 Sip Height Medium SamInterval 0 Min Autostandby 2 Hr PreCal 0 Min TT StartPos 0 PostCal 0 Min TT in Run 0 Purge Time 0 Sec TT FluidDet ON RS 232 MONITOR Baud rate 9600 MonStation 1 Data Seven bit SamInterval 0 Min Stop One bit PreCal 0 Min Parity Even PostCal 0 Min Handshake RTS CTS Purge Time 0 Sec XON char 17 RS 232 XOFF char 19 B...

Page 152: ...Report CALIBRATION REPORT ID 123456789 05 03 B L Lactate Unstable B L Lactate 4 82 g L IB current 1 79 nA IB 2 11 nA PL current 11 63 nA PL current 18 65 nA FB current 1 51 nA Slope 0 69 nA min Base shift 2 65 End Point 30 Sec Slope 1 42 nA min W Dextrose 5 38 g L End Point 30 Sec IB current 0 88 nA Cal shift 3 64 PL current 15 96 nA W Dextrose 2 50 g L Slope 0 39 nA min IB current 0 88 nA End Poi...

Page 153: ...D 2 ...

Page 154: ...this equipment does cause interference to radio or television reception which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures reorient the receiving antenna relocate the computer with respect to the receiver move the computer away from the receiver plug the computer into a different outlet so t...

Page 155: ...E 2 ...

Page 156: ... product or vi modification of the product in any way not expressly authorized by YSI THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE YSI s LIABILITY UNDER THIS WARRANTY IS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT AND THIS SHALL BE YOUR SOLE AND EXCLUSIVE REMEDY FOR ANY DEFECTIVE PRODUCT COVE...

Page 157: ...he equipment Disposition costs will be the responsibility of the sender When service is required either at the user s facility or at YSI the following steps must be taken to insure the safety of our service personnel 1 In a manner appropriate to each device decontaminate all exposed surfaces including any containers 70 isopropyl alcohol or a solution of 1 4 cup bleach to 1 gallon tap water are sui...

Page 158: ...arge carton preferably the original and surround the product completely with packing material 5 Insure for the replacement value of the product Cleaning Certificate Organization Department Address City State Zip Country Phone Model No of Device Lot Number Contaminant if known Cleaning Agent s used Radioactive Decontamination Certified Answer only if there has been radioactive exposure Yes No Clean...

Page 159: ...F 4 ...

Page 160: ...G 1 Figure G 1 Line Power Cord and Plug Wiring ...

Page 161: ...G 2 ...

Page 162: ...ach Decontamination Procedures Remove and discard all tubing New tubing is provided in preventive maintenance kit Empty waste bottle and wash with disinfecting agent Remove Sample Chamber Sipper Test Tube Holder and probes according to instructions Thoroughly clean with disinfecting agent then rinse with warm water Remove probes and discard membranes Clean probes with isopropanol only rinse with w...

Page 163: ...H 2 ...

Page 164: ...sm is equipped with contact sensing circuitry for the purposes of fluid detection and human safety This circuitry may be susceptible to ESD ESD to the sipper needle may cause instrument malfunction 3 Users should note that RS232 communications may be susceptible to ESD Data corruption or serial communications failure may occur If malfunction occurs users may restore proper operation by resetting c...

Page 165: ...1725 Brannum Lane Yellow Springs Ohio 45387 USA 062521 937 767 7241 800 659 8895 Fax 937 767 8058 A23781L Info ysi com www YSI com July 2000 2000 YSI Incorporated ...

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