Waters Xevo TQ-XS Mass, Overview And Maintenance Manual

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Table 1–1: MS operating modes:
Operating mode MS1 Collision cell MS2
MS Pass all masses Resolving (scanning)
SIR Pass all masses Resolving (static)
MS1 Resolving (scanning) Pass all masses
In MS mode, the instrument can acquire data at scan speeds as high as 20,000 Da/s. Use this
mode for instrument tuning and calibration before MS/MS analysis. See the mass spectrometer’s
online Help for further information.
Use the selected ion recording (SIR) mode for quantitation when you cannot find a suitable
fragment ion to perform a more specific multiple reaction monitoring (MRM) analysis (see
MS/MS
operating modes for further details) . In SIR and MRM modes, neither quadrupole is scanned,
therefore no spectrum (intensity versus mass) is produced. The data obtained from SIR or MRM
analyses derive from the chromatogram plot (specified mass intensity versus time).
1.7 MS/MS operating modes
The following table shows the MS/MS operating modes.
Table 1–2: MS/MS operating modes:
Operating mode MS1 Collision cell MS2
Product (daughter)
ion spectrum Static (at precursor
mass) Fragment precursor ions
and pass all masses Scanning
Precursor (parent)
ion spectrum Scanning Static (at product
mass)
MRM Static (at precursor
mass) Static (at product
mass)
Constant neutral
loss spectrum Scanning
(synchronized with
MS2) Scanning
(synchronized with
MS1)
Constant neutral
gain spectrum Scanning
(synchronized with
MS2) Scanning
(synchronized with
MS1)
ScanWave
daughter scan Static (at precursor
mass) Scanning
(synchronized with
collision cell)
June 9, 2016, 715004990 Rev. A
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