WATERS CORPORATION TQ Operator'S Manual Download Page 13

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December 6, 2012, 71500126802IVD Rev. C

xiii

Copyright notice ................................................................................................... ii

Trademarks ............................................................................................................ ii

Contacting Waters ............................................................................................... iii

Safety considerations .......................................................................................... iv

Operating this instrument .............................................................................. viii

Applicable symbols .......................................................................................... viii
Intended use........................................................................................................ ix
Calibrating .......................................................................................................... ix
Quality control ..................................................................................................... x

ISM classification ................................................................................................. xi

EC authorized representative .......................................................................... xii

1 Waters TQ Detector ................................................................................ 19

Overview .............................................................................................................. 20

Waters TQ Detector ...........................................................................................  20
ACQUITY TQD UPLC/MS system ...................................................................  22
Software and data system .................................................................................  22

Ionization techniques and source probes .................................................... 24

Electrospray ionization (ESI) ............................................................................ 24

Table of Contents

Summary of Contents for TQ

Page 2: ...ctions INSIGHT and Waters are registered trademarks of Waters Corporation ACQUITY IntelliStart IonSABRE MassLynx T Wave UPLC and THE SCIENCE OF WHAT S POSSIBLE are trademarks of Waters Corporation Nal...

Page 3: ...tact information Contacting medium Information Internet The Waters Web site includes contact information for Waters locations worldwide Visit www waters com Telephone and fax From the USA or Canada ph...

Page 4: ...bar 100 psi during analyses that require flammable solvents Connect to the LC output with a gas fail connector to stop the LC solvent if the nitrogen supply fails Warning To confirm the integrity of t...

Page 5: ...ector high temperature hazard Warning To avoid burn injuries avoid touching the source enclosure with your hand when operating or servicing the instrument NEBULIZER NEBULIZER APPI APPI PROBE PROBE HV...

Page 6: ...003 Cet appareil num rique de la classe A est conforme la norme NMB 003 Electrical power safety notice Do not position the instrument so that it is difficult to operate the disconnecting device Safet...

Page 7: ...ipment misuse notice If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired Safety advisories Consult Appendix A for a comprehe...

Page 8: ...plies with all applicable European Community directives Australia C Tick EMC Compliant Confirms that a manufactured product complies with all applicable United States and Canadian safety requirements...

Page 9: ...calibration methods using at least five standards to generate a standard curve The concentration range for standards must cover the entire range of quality control samples typical specimens and atypic...

Page 10: ...When analyzing samples from a complex matrix such as serum plasma whole blood and so on note that the matrix components can adversely affect LC MS results enhancing or suppressing ionization To minim...

Page 11: ...in commercial that is nonresidential locations and can be directly connected to a low voltage power supply network In a domestic environment the equipment may cause radio interference You may need to...

Page 12: ...12 71500126802IVD Rev C EC authorized representative Waters Corporation Micromass UK Ltd Floats Road Wythenshawe Manchester M23 9LZ United Kingdom Telephone 44 161 946 2400 Fax 44 161 946 2480 Contact...

Page 13: ...er safety notice vi Safety hazard symbol notice vi Equipment misuse notice vii Safety advisories vii Operating this instrument viii Applicable symbols viii Intended use ix Calibrating ix Quality contr...

Page 14: ...5 Starting the instrument 36 Configuring IntelliStart 38 Verifying the instrument s state of readiness 38 Tuning and calibration information 38 Running the instrument at high flow rates 38 Monitoring...

Page 15: ...ing the exhaust trap bottle 63 Emptying the roughing pump exhaust liquid trap bottle 65 Gas ballasting the roughing pump 68 Gas ballasting a pump fitted with a screwdriver operated gas ballast valve 6...

Page 16: ...ource assembly 106 Replacing the ESI probe tip 108 Replacing the ESI probe sample capillary 109 Replacing the ion block source heater 116 Replacing the source assembly seals 121 Removing the source en...

Page 17: ...vacuum connections 158 Connecting the oil filled roughing pump 159 Making the electrical connections for a roughing pump with an external relay box 164 Making the electrical connections for a roughing...

Page 18: ...2 71500126802IVD Rev C Connecting to the electricity source 187 C Materials of Construction and Compliant Solvents 189 Preventing contamination 190 Items exposed to solvent 190 Solvents used to prepar...

Page 19: ...uding its controls and gas and plumbing connections Contents Topic Page Overview 20 Ionization techniques and source probes 24 Ion optics 25 MS operating modes 26 MS MS operating modes 27 Sample inlet...

Page 20: ...eric pressure ionization API mass spectrometer Designed for routine UPLC MS MS analyses in quantitative and qualitative applications it can operate at fast acquisition speeds compatible with ultra per...

Page 21: ...les simplified setup of the system for use in routine analytical and open access applications See Software and data system on page 1 22 The IntelliStart Fluidics system is built into the instrument It...

Page 22: ...or Controlling Contamination in LC MS Systems part number 715001307 You can find the documents on http www waters com Software and data system The instrument is controlled by MassLynx mass spectromet...

Page 23: ...Overview December 6 2012 71500126802IVD Rev C 23 the system and its modules The ACQUITY UPLC Console functions independently of the MassLynx software and does not recognize or control the data systems...

Page 24: ...aerosol undergo a reduction in size solvent evaporation As solvent continues to evaporate the charge density increases until the droplet surfaces eject ions ion evaporation The ions can be singly or m...

Page 25: ...o their mass to charge ratios 4 The mass separated ions pass into the T Wave collision cell where they either undergo collision induced dissociation CID or pass to the second quadrupole Any fragment i...

Page 26: ...useful tool for instrument tuning and calibration before MS MS analysis and for fault diagnosis See the instrument online Help for further information on optimizing the TQ Detector for fast MS scannin...

Page 27: ...e Typical applications Product ion mode is typically used for the following applications Method development for MRM screening studies MS MS operating modes Operating mode MS1 Collision cell MS2 Produc...

Page 28: ...m product scan data by scanning for all the precursors of a common product ion Multiple reaction monitoring mode MRM mode is a highly selective MS MS equivalent of Selected Ion Recording SIR Because b...

Page 29: ...al loss mode detects the loss of a specific neutral fragment or functional group from an unspecified precursor s The scans of MS1 and MS2 are synchronized When MS1 transmits a specific precursor ion M...

Page 30: ...leak sensor stops system flow when its optical sensor detects about 1 5 mL of accumulated leaked liquid in its surrounding reservoir At the same time the ACQUITY UPLC Console displays an error message...

Page 31: ...ACN 065444751 1 2 3 4 5 6 7 8 9 10 Anal og Notused St op Fl ow Swi t ch 2 G r ound G r ound O ut O ut 1 2 3 4 5 6 7 8 9 10 I nj ectS t ar t Event Swi t ch 3 Swi t ch 4 G r ound G r ound I n I n O ut...

Page 32: ...ect or combined infusion to enable optimization at analytical flow rates The system incorporates a multi position valve with these attributes An input connection from an external LC column An input co...

Page 33: ...development is automatically performed by the software IntelliStart Fluidics configuration requirements can be set in the system console You can edit the parameters frequency and extent of the automat...

Page 34: ...1 Waters TQ Detector 34 December 6 2012 71500126802IVD Rev C...

Page 35: ...for Operation This chapter describes how to start and shut down the instrument Contents Topic Page Starting the instrument 36 Preparing the IntelliStart Fluidics system 40 Rebooting the instrument 45...

Page 36: ...d oil free with a purity of at least 95 Regulate the supply at 600 to 690 kPa 6 0 to 6 9 bar 90 to 100 psi For more information on connections see the figure on page 31 2 Ensure that the collision gas...

Page 37: ...Operate LED remain off 6 Start the MassLynx software 7 Click IntelliStart in the MassLynx main window s lower left hand corner to launch IntelliStart Result The TQ Detector s console appears The instr...

Page 38: ...Properties 5 Click OK Verifying the instrument s state of readiness When the instrument is in good operating condition the power and Operate LEDs show constant green You can view any error messages i...

Page 39: ...t indicate its operational status Power LED The power LED to the top left hand side of the instrument s front panel indicates when the instrument is powered on or powered off Operate LED The Operate L...

Page 40: ...lvent manifold drip tray Required material Chemical resistant powder free gloves To install the solvent manifold drip tray Install the solvent manifold drip tray as shown below Warning The solvent man...

Page 41: ...stant powder free gloves To install the reservoir bottles 1 Remove the reservoir bottle caps 2 Screw the reservoir bottles onto the instrument as shown below To install low volume vials 1 If a standar...

Page 42: ...t 3 Screw the low volume vial into the adaptor Diverter valve positions Column and syringe in home position after power up After power up the flow path between the column and waste is open The syringe...

Page 43: ...the LC position the flow path between the LC and probe is open and the flow path between the syringe and waste is also open Infusion position Column LC LC Waste Probe Syringe Idle Reservoir A Reservoi...

Page 44: ...position In the waste position both the LC flow and the infusion syringe flow are directed to waste The syringe mode can be only static or dispensing that is never drawing Column Combined LC Waste Pro...

Page 45: ...ize carryover Rebooting the instrument Reboot the instrument when any of these conditions applies The Tune window fails to respond MassLynx software fails to initialize Immediately following a softwar...

Page 46: ...nstances put the mass spectrometer in Standby mode See the online Help for details Emergency instrument shutdown To shut down the instrument in an emergency 1 Operate the power button on the front of...

Page 47: ...71500126802IVD Rev C 47 3 ESI Operation This chapter describes how to prepare the instrument for ESI electrospray ionization Contents Topic Page Introduction 48 Installing the ESI probe 48 Removing t...

Page 48: ...r instruction see page 24 Installing the ESI probe Probe compatibility warning label Required material Chemical resistant powder free gloves Warning On instruments bearing the probe compatibility warn...

Page 49: ...ons ESI probe and source can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Warning To avoid electric shock...

Page 50: ...acts on the ESI probe align with the probe adjuster assembly contacts and carefully slide the ESI probe into the hole in the probe adjuster assembly 5 Secure the ESI probe by tightening the 2 thumbscr...

Page 51: ...different ID are supplied with the instrument Requirement If you are replacing the tubing supplied with the instrument you must minimize the length of the tube connecting the diverter valve to the ES...

Page 52: ...probe to the probe adjuster assembly 8 Carefully remove the ESI probe from the probe adjuster assembly 9 If available fit the protective sleeve to the ESI probe tip 10 Close the instrument s access d...

Page 53: ...rce 59 Operating the source isolation valve 60 Removing O rings and seals 62 Cleaning the instrument s exterior 63 Emptying the exhaust trap bottle 63 Emptying the roughing pump exhaust liquid trap bo...

Page 54: ...ance Procedures 54 December 6 2012 71500126802IVD Rev C Maintaining the instrument air filters 131 Replacing the roughing pump oil 135 Replacing the roughing pump s oil demister element 138 Contents T...

Page 55: ...rap bottle in the roughing pump exhaust line Check daily empty as required See page 65 Gas ballast the roughing pump ESI weekly See page 68 Inspect and adjust the roughing pump oil level Weekly See pa...

Page 56: ...h you control the system The service agent software automatically and securely captures and sends information about the support needs of your system directly to Waters If you encounter a performance i...

Page 57: ...d allow approximately 5 minutes to save the service profile Result A zip file containing your Connections INSIGHT profile is forwarded to Waters customer support for review Tip Saving a service profil...

Page 58: ...de the instrument ensure that the instrument is in Standby mode before commencing any maintenance Warning The probe and source can be hot To avoid burn injuries take great care while working with thes...

Page 59: ...urce To prepare the instrument 1 In the Instrument Console click Stop Flow to stop the LC flow or if column flow is required divert the LC flow to waste as follows a In the Instrument Console system t...

Page 60: ...asing both spring clips and lowering the door towards you Warning The source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves w...

Page 61: ...urce enclosure door and secure it with both spring clips 3 Close the instrument s access door Warning The source components can be contaminated with biohazardous and or toxic materials Always wear che...

Page 62: ...removal kit To remove an O ring 1 Use the forked end of tool 1 to impale the O ring or seal 2 Pull the O ring or seal from its groove if necessary use tool 2 as an aid 3 Dispose of the O ring or seal...

Page 63: ...xhaust trap bottle Check the exhaust trap bottle in the instrument exhaust line daily and empty it before it is completely full Instrument exhaust trap bottle Required materials Chemical resistant pow...

Page 64: ...ion gas flow 8 In the ACQUITY UPLC Console click Start Flow to start the LC flow Warning The waste liquid in the exhaust trap bottle comprises ACQUITY UPLC solvents and analytes Always wear chemical r...

Page 65: ...etector liquid to ensure that there are no leaks at the exhaust trap bottle cap Emptying the roughing pump exhaust liquid trap bottle Check the liquid trap bottle in the roughing pump exhaust line dai...

Page 66: ...tor liquid To empty the exhaust liquid trap bottle 1 Close the source isolation valve see page 60 Warning The liquid in the roughing pump liquid trap bottle can be contaminated with analyte accumulate...

Page 67: ...in the upright position 6 Open the source isolation valve see page 60 7 Use snoop or equivalent leak detector liquid to ensure that there are no leaks at the liquid trap bottle cap Warning The waste...

Page 68: ...ballast the roughing pump when these conditions apply With ESI operation once a week When the roughing pump oil appears cloudy When the vacuum pressure is higher than normal When condensate forms in t...

Page 69: ...osition 2 Run the pump for 30 to 60 minutes Tip It is normal for the roughing pump temperature to increase during ballasting To maintain an ambient temperature of 40 C 104 F where the pump is located...

Page 70: ...tion 2 Run the pump for 30 to 60 minutes Tip It is normal for the roughing pump temperature to increase during ballasting To maintain an ambient temperature of 40 C 104 F where the pump is located ens...

Page 71: ...at it is at or near the MAX level when the pump is not operating Tip The oil level in the sight glass is lower when the roughing pump is running than when it is stopped When the pump is running the oi...

Page 72: ...rolled by the O ring groove depth in the plug Increased torque does not improve the plug seal it only makes the plug difficult to remove later 6 Start the instrument see page 36 Tips After you add oil...

Page 73: ...led oil smells slightly Cleaning the source components Clean the sample cone and gas cone when these conditions apply The sample cone and gas cone are visibly fouled LC and sample related causes for d...

Page 74: ...The source components can be contaminated with biohazardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Warning To avoid electric shock ens...

Page 75: ...cone gas assembly connection tube 3 Grasp the cone gas assembly connection tube and use it as a lever to rotate the cone gas assembly 90 degrees moving the connection tube from the vertical to the hor...

Page 76: ...2012 71500126802IVD Rev C 4 Slide the cone gas assembly out of the ion block assembly Caution Do not open the isolation valve at any time when the cone gas assembly has been removed from the ion bloc...

Page 77: ...d with the instrument This tool is used in a similar manner to that fitted to the source enclosure door Warning The cone gas assembly can be contaminated with biohazardous and or toxic materials Alway...

Page 78: ...ves Appropriately sized glass vessels in which to completely immerse components when cleaning Use only glassware not previously cleaned with surfactants HPLC grade or better methanol HPLC grade or bet...

Page 79: ...taining water and then placing the vessels in the ultrasonic bath for 20 minutes b Displace the water by immersing the components in separate glass vessels containing methanol and then placing the ves...

Page 80: ...present do as follows a Use the wash bottle containing 1 1 methanol water to rinse the component over the large beaker b Blow dry the component with inert oil free gas 7 Inspect each component for pe...

Page 81: ...assembly 1 Carefully fit the sample cone into the gas cone 2 Fit a new O ring into the groove created between the sample cone and gas cone Caution To avoid recontaminating the cone gas assembly wear...

Page 82: ...ardous and or toxic materials Always wear chemical resistant powder free gloves while performing this procedure Warning To avoid puncture wounds take great care while working with the source enclosure...

Page 83: ...embly into the ion block assembly 3 Grasp the cone gas assembly connection tube and use it as a handle to rotate the cone gas assembly 90 degrees moving the connection tube from the horizontal to the...

Page 84: ...3 Open the source enclosure door by releasing both spring clips and lowering the door towards you 4 Close the source isolation valve see page 60 5 Disconnect the PTFE tube from the cone gas assembly...

Page 85: ...2 71500126802IVD Rev C 85 6 Use the 6 mm Allen wrench to unscrew and remove the 2 ion block assembly securing screws and associated washers 7 Remove the ion block assembly from the PEEK ion block supp...

Page 86: ...ion block assembly 1 Ensure that the isolation valve is closed 2 Grasp the cone gas assembly connection tube and use it as a lever to rotate the cone gas assembly 90 degrees Warning The ion block asse...

Page 87: ...en wrench to loosen the 4 ion block cover plate captive securing screws 5 Remove the ion block cover plate Caution To ensure correct operation of the ion block assembly after reassembly the gas cone p...

Page 88: ...to carefully remove the isolation valve O ring see page 62 8 Dispose of the isolation valve O ring in accordance with local environmental regulations Warning The isolation valve O ring can be contami...

Page 89: ...the 2 set screws securing the heater cartridge to the PEEK terminal block are fitted use the 1 5 mm Allen wrench to loosen them 10 Use the 2 5 mm Allen wrench to loosen the captive PEEK terminal block...

Page 90: ...eedle nose pliers to gently grasp the heat shrink tubing on the heater cartridge assembly and slide it and the PEEK terminal block out of the ion block Caution To avoid damaging the heater cartridge a...

Page 91: ...refully remove the cover seal from the ion block see also page 62 14 Dispose of the cover seal in accordance with local environmental regulations Warning The cover seal can be contaminated with biohaz...

Page 92: ...king plug and associated seal 16 Dispose of the blanking plug seal in accordance with local environmental regulations Warning The blanking plug seal can be contaminated with biohazardous and or toxic...

Page 93: ...w 18 Remove the extraction cone retainer and extraction cone from the ion block Caution Take great care not to damage the extraction cone aperture when removing the extraction cone from the ion block...

Page 94: ...ls Chemical resistant powder free gloves Appropriately sized glass vessels in which to completely immerse components when cleaning Use only glassware not previously cleaned with surfactants HPLC grade...

Page 95: ...ng the vessels in the ultrasonic bath for 10 minutes 4 Carefully remove the components from the vessels and blow dry them using inert oil free gas 5 Inspect each component for persisting contamination...

Page 96: ...r To assemble the ion block assembly 1 Fit the extraction cone seal to the ion block 2 Fit the extraction cone retainer to the extraction cone 3 Fit the extraction cone retainer and extraction cone to...

Page 97: ...the cover seal and extraction cone seal are free from dirt and debris Tip If contamination is present use 1 1 methanol water applied to a lint free cloth to carefully clean the grooves 11 Fit a new co...

Page 98: ...lock from the PEEK ion block support on the instrument 19 Fit the ion block cover plate to the ion block assembly and then use the 2 5 mm Allen wrench to tighten the 4 ion block cover plate captive se...

Page 99: ...rce enclosure door by releasing both spring clips and lowering the door towards you 3 Remove the side flange from the source enclosure Rationale This step allows you to see the ion block assembly as y...

Page 100: ...osition during installation If this is the case remove the ion block assembly refit the extraction cone and reinstall the ion block assembly on the PEEK ion block support 7 When you are satisfied with...

Page 101: ...l resistant powder free gloves 3 mm Allen wrench O ring removal kit To remove the ion block assembly ion block support and hexapole 1 Remove the ion block assembly from the PEEK ion block support see...

Page 102: ...environmental regulations 6 Carefully grasp the source hexapole assembly and remove it from the adaptor housing Warning The O rings can be contaminated with biohazardous and or toxic materials Ensure...

Page 103: ...methanol Ultrasonic bath Source of oil free inert gas nitrogen or helium for drying air drying optional HPLC grade or better 1 1 methanol water Wash bottle containing HPLC grade or better 1 1 methanol...

Page 104: ...mersed completely 5 Place the vessel in the ultrasonic bath for 30 minutes 6 Carefully remove the hexapole assembly from the vessel and blow dry it using inert oil free gas Caution To avoid vibration...

Page 105: ...rsisting contamination If contamination is present do as follows a Use the wash bottle containing methanol to rinse the source hexapole assembly over the large beaker b Blow dry the hexapole assembly...

Page 106: ...rt O rings are free from dirt and debris Tip If contamination is present use 1 1 methanol water applied to a lint free cloth to carefully clean the grooves 3 Fit new O rings to the PEEK ion block supp...

Page 107: ...and tighten the 3 PEEK ion block support securing screws Tip Look through the PEEK ion block support as you tighten the securing screws Ensure that neither hexapole spring buckles and touches a hexapo...

Page 108: ...d tighten the new probe tip to the probe 5 Adjust the probe tip so that the fully extended capillary when the probe nebulizer adjuster knob is fully screwed down protrudes by approximately 1 to 1 5 mm...

Page 109: ...h wrench 7 16 inch wrench Flat blade screwdriver Needle nose pliers LC pump HPLC grade or better 1 1 acetonitrile water To replace the capillary 1 Remove the probe from the source see page 52 Warning...

Page 110: ...s 110 December 6 2012 71500126802IVD Rev C 2 Use the screwdriver to remove the 2 probe end cover retaining screws 3 Use the 1 5 mm Allen wrench to loosen the 3 set screws on the LC PEEK union 2 retain...

Page 111: ...Replacing the ESI probe sample capillary December 6 2012 71500126802IVD Rev C 111 4 Remove the end cover 5 Use the 6 mm wrench to remove the probe tip End cover TP03007 Probe tip...

Page 112: ...ry PTFE liner ferrule assembly and seal in accordance with local environmental regulations 10 Use the needle nose pliers to remove the conductive sleeve from the inner bore of the probe assembly fitti...

Page 113: ...end of the LC union 15 Lightly screw the coupling into the LC union so that it does not restrict movement of the capillary 16 Slide a compression screw and ferrule onto a piece of 1 16 0 0025 bore 5M...

Page 114: ...n and then repeat the leak test 21 When performing the leak test check the backpressure on the LC pump which will be relatively high if the capillary is blocked If this is the case replace the capilla...

Page 115: ...e the probe tip and then screw down until the capillary protrudes approximately 0 5 mm from the end of the tip 27 Fit the probe end cover 28 Fit and tighten the two probe end cover retaining screws 29...

Page 116: ...lock source heater 1 Remove the ion block assembly from the instrument see page 84 2 Ensure that the isolation valve is closed Warning The capillary and ferrule can be contaminated with biohazardous a...

Page 117: ...tive screws securing the ion block cover plate 4 Remove the ion block cover plate 5 Use the 1 5 mm Allen wrench to remove the 2 screws securing the heater wires to the PEEK terminal block TP02649 Ion...

Page 118: ...nose pliers to carefully swing the ring terminal tags out of the terminal block 7 If the 2 set screws securing the heater cartridge to the PEEK terminal block are fitted use the 1 5 mm Allen wrench to...

Page 119: ...PEEK terminal block are fitted use the 1 5 mm Allen wrench to fit and tighten them 12 Use the needle nose pliers to position the 2 heater wire ring tags fully down on the PEEK block terminals Caution...

Page 120: ...fit and tighten the 2 screws securing the heater wires to the PEEK terminal block 14 Fit the ion block cover plate to the ion block assembly and then use the 2 5 mm Allen wrench to tighten the 4 capti...

Page 121: ...Pressure Test Unit Operator s Guide Removing the source enclosure from the instrument Required materials Chemical resistant powder free gloves 5 mm Allen wrench To remove the source enclosure 1 Vent a...

Page 122: ...connection on the front panel 6 Disconnect the 2 pin connector at the rear of the source enclosure 7 Use the 5 mm Allen wrench to loosen the 3 captive source enclosure securing screws 8 Remove the sou...

Page 123: ...o the source enclosure 2 Remove the probe adjuster assembly from the source enclosure 3 Unscrew and remove the 3 thumbscrews and door spring clip that secure the source enclosure side flange to the so...

Page 124: ...y 1 Use the O ring removal kit to carefully remove the probe adjuster assembly probe seal from the probe adjuster assembly see page 62 2 Use the O ring removal kit to carefully remove the following se...

Page 125: ...flange seal 3 Open the source enclosure door by releasing both spring clips and lowering the door towards you Caution Do not apply any downward force to the source enclosure door while the door is op...

Page 126: ...enclosure door 7 Remove the glass from the source enclosure door 8 Use the O ring removal kit to carefully remove the door glass seal from the source enclosure door 9 Dispose of all the seals in accor...

Page 127: ...losure door 5 Fit and tighten the 4 screws securing the glass retaining clips to the source enclosure door 6 Fit the new source enclosure door seal to the source enclosure door 7 Close the source encl...

Page 128: ...osure 1 Fit the source enclosure side flange to the source enclosure 2 Fit and tighten the 3 thumbscrews that secure the source enclosure side flange to the source enclosure 3 Fit the probe adjuster a...

Page 129: ...Connect the PTFE tubing to the desolvation gas connection at the instrument s front panel 5 Connect the probe s electrical connection at the instrument s front panel 6 Close the source enclosure door...

Page 130: ...36 9 Perform a source pressure test as described in the Waters Micromass Source Pressure Test Unit Operator s Guide Warning To confirm the integrity of the source exhaust system a source pressure test...

Page 131: ...strument s lower bezel Required materials T10 TORX driver Vacuum cleaner To clean the air filter inside the instrument s lower bezel 1 Use the T10 TORX driver to remove the 5 screws that secure the lo...

Page 132: ...air filter by vacuuming replace it with a new filter Required materials T10 TORX driver TQ Detector lower bezel air filter To replace the air filter inside the lower bezel 1 Remove the 5 screws that s...

Page 133: ...he T10 TORX driver to fit and tighten the 4 screws securing the air filter and frame to the inside of the lower bezel 6 Reinstall the lower bezel Cleaning the air filter behind the source probe Requir...

Page 134: ...1500126802IVD Rev C Air filter tab 3 Use a vacuum cleaner to clean the air filter Air filter removed from behind the source probe 4 Reinstall the air filter 5 Fit the probe to the source see page 48 R...

Page 135: ...he figure on page 134 3 Remove the air filter from the air filter frame and dispose of it Removing air filter from frame 4 Insert the new air filter in the air filter frame 5 Reinstall the air filter...

Page 136: ...d shut down the instrument see the mass spectrometer s online Help for details 3 Allow the roughing pump to cool 4 Place the container for used oil under the pump s drain plug 5 Use the 8 mm Allen wre...

Page 137: ...is controlled by the O ring groove depth in the plug Increased torque does not improve the plug seal it only makes the plug difficult to remove later 11 Using the funnel pour all the oil from the 1 L...

Page 138: ...plug will run down and drip off the pump once the pump reaches operating temperature When the pump begins to run at normal operating temperature spilled oil smells slightly Replacing the roughing pump...

Page 139: ...bolts securing the exhaust flange to the roughing pump Warning The pump oil can be contaminated with analyte accumulated during normal operation Always wear chemical resistant powder free gloves when...

Page 140: ...C 4 Using both hands carefully remove the exhaust flange and oil demister element from the roughing pump 5 Use the 10 mm wrench to remove the nut that secures the oil demister element to the exhaust...

Page 141: ...emove the exhaust flange from the oil demister element 7 Remove the spring from the oil demister element 8 Dispose of the oil demister element in accordance with local environmental regulations Warnin...

Page 142: ...o fit and tighten the nut that secures the oil demister element to the exhaust flange Warning The pump oil can be contaminated with analyte accumulated during normal operation Always wear chemical res...

Page 143: ...oth hands carefully fit the oil demister element and exhaust flange to the roughing pump 5 Use the 6 mm Allen wrench to fit the 4 bolts securing the exhaust flange to the roughing pump 6 Start the ins...

Page 144: ...4 Maintenance Procedures 144 December 6 2012 71500126802IVD Rev C...

Page 145: ...responding user guides also include the hazard symbols with accompanying text statements describing the hazards and telling you how to avoid them This appendix presents all the safety symbols and stat...

Page 146: ...nt component Such risks include burn injuries electric shocks ultraviolet radiation exposures and others When the following symbols appear in a manual s narratives or procedures their accompanying tex...

Page 147: ...n pose a serious health threat Warning Risk of tipping Warning Risk of explosion Warning Pressurized nonmetallic or polymer tubing can burst Observe these precautions when working around such tubing W...

Page 148: ...that enclosed space Ensure that the nitrogen supply pressure never falls below 690 kPa 6 9 bar 100 psi during an analysis in which flammable solvents are used Also ensure a gas fail connection is conn...

Page 149: ...ctivated microorganisms and other biological materials To avoid infection with these agents assume that all biological fluids are infectious observe Good Laboratory Practices and consult your organiza...

Page 150: ...ementation peut annuler le droit de l utilisateur exploiter l quipement Achtung Jedwede nderungen oder Modifikationen an dem Ger t ohne die ausdr ckliche Genehmigung der f r die ordnungsgem e Funktion...

Page 151: ...nez toute flamme se trouvant proximit de l instrument Evitez d utiliser des tubes s v rement d form s ou endommag s Evitez d utiliser des tubes non m talliques avec du t trahydrofurane THF ou de l aci...

Page 152: ...do provocano rigonfiamenti nei tubi non metallici riducendo notevolmente la pressione di rottura dei tubi stessi Advertencia se recomienda precauci n cuando se trabaje con tubos de pol mero sometidos...

Page 153: ...r le mat riel risque d tre d fectueuses Vorsicht Der Benutzer wird darauf aufmerksam gemacht dass bei unsachgem er Verwenddung des Ger tes die eingebauten Sicherheitseinrichtungen unter Umst nden nich...

Page 154: ...nd handling symbols Electrical symbols These can appear in instrument user manuals and on the instrument s front or rear panels Electrical power on Electrical power off Standby Direct current Alternat...

Page 155: ...r 6 2012 71500126802IVD Rev C 155 Handling symbols These handling symbols and their associated text can appear on labels affixed to the outer packaging of Waters instrument and component shipments Kee...

Page 156: ...A Safety Advisories 156 December 6 2012 71500126802IVD Rev C...

Page 157: ...t or remove it from service contact Waters Technical Service for recommended cleaning flushing and packaging procedures See page 56 Contents Topic Page External wiring and vacuum connections 158 Conne...

Page 158: ...d G r ound O ut O ut 1 2 3 4 5 6 7 8 9 10 I nj ectS t ar t Event Swi t ch 3 Swi t ch 4 G r ound G r ound I n I n O ut O ut O ut RS 232 RS 232 ETHERNET ETHERNET API Gas API Gas COLLISION GAS COLLISION...

Page 159: ...p bottle included in the startup kit Elbows included in the Waters Rough Pump Connect Kit NW25 tee included in the startup kit NW25 center rings included in the startup kit NW25 clamps included in the...

Page 160: ...t powder free gloves when performing this procedure Caution To ensure correct operation of the roughing pump the pump must be installed within 1 degree of horizontal The area where the roughing pump i...

Page 161: ...to install the clamps 5 Connect the opposite ends of the two lengths of vacuum hose in step 4 to the two 1 inch OD straight vacuum ports on the instrument s rear panel Secure the hose ends with clamps...

Page 162: ...kit 8 Connect a length of 12 7 mm clear PVC exhaust tubing to an elbow and connect the elbow to the other fitting on the exhaust trap bottle The exit line of the exhaust trap bottle can be at any elev...

Page 163: ...x see page 164 If your roughing pump does not have an external relay box see page 165 Caution The instrument requires two separate exhaust systems one for nitrogen the other for the roughing pump Vent...

Page 164: ...connector on the instrument s rear panel 01757 U S A V 200 240 V 200 240 Hz 50 60 Hz 50 60 V VA 900 ACN 065444751 1 2 3 4 5 6 7 8 9 10 Anal og Notused St op Fl ow Sw i t ch 2 G r ound G r ound O ut O...

Page 165: ...ing pump d c connector to the pump connector on the instrument s rear panel 01757 U S A V 200 240 V 200 240 Hz 50 60 Hz 50 60 V VA 900 ACN 065444751 1 2 3 4 5 6 7 8 9 10 Anal og Notused St op Fl ow Sw...

Page 166: ...o minimize operating noise unless the pump is installed in a cabinet that includes sound dampening and ventilation Required materials Chemical resistant powder free gloves 7 mm nut driver 8 mm Allen w...

Page 167: ...mp Connect Kit 1 inch ID vacuum hose included in the Waters Rough Pump Connect Kit Isolation valve included in the Alcatel pump kit External silencer included in the Alcatel pump kit Noise reduction c...

Page 168: ...B External Connections 168 December 6 2012 71500126802IVD Rev C 6 Install the noise reduction cover TP02996 DN 25 nipple DN 40 nipple Clamps...

Page 169: ...ect operation of the roughing pump the pump must be installed within 1 degree of horizontal The area where the roughing pump is located must have an ambient temperature of 15 to 40 C 59 to 104 F To en...

Page 170: ...all the clamps 4 Connect the opposite ends of the two lengths of vacuum hose in step 3 to the two 1 inch OD straight vacuum ports on the instrument s rear panel Secure the hose ends by installing 2 cl...

Page 171: ...nection with a clamp 8 Connect an approximately 300 mm 12 inch length of 12 7 mm clear PVC exhaust tubing to the DN25 reducing nipple securing the tubing with a hose clamp 9 Connect an elbow to the ot...

Page 172: ...ap bottle The exit line of the exhaust trap bottle can be at any elevation 11 Secure the exhaust trap bottle in a conspicuous location 12 Route the open end of the exhaust tubing to a suitable exhaust...

Page 173: ...ct the power cable and power on the instrument 01757 U S A V 200 240 V 200 240 Hz 50 60 Hz 50 60 V VA 900 ACN 065444751 1 2 3 4 5 6 7 8 9 10 Anal og Notused St op Fl ow Swi t ch 2 G r ound G r ound O...

Page 174: ...sharp knife to cut a 3 8 to 5 0 cm 1 5 to 2 inch length of 6 mm PTFE tubing 2 Connect this piece of tubing to one end of the nitrogen supply in line filter 3 Connect the remaining length of the 6 mm...

Page 175: ...50 60 V VA 900 ACN 065444751 1 2 3 4 5 6 7 8 9 10 Anal og Notused St op Fl ow Swi t ch 2 G r ound G r ound O ut O ut 1 2 3 4 5 6 7 8 9 10 I nj ectS t ar t Event Swi t ch 3 Swi t ch 4 G r ound G r ound...

Page 176: ...the 1 8 inch Swagelok nut and ferrule to connect the 1 8 inch stainless steel tube to the collision cell gas inlet on the rear of the instrument see the figure on page 175 2 Use the wrench to tighten...

Page 177: ...m vacuum of 0 20 kPa 2 mbar 0 03 psi below atmospheric pressure negative pressure The exhaust connections can be contaminated with biohazardous and or toxic materials Always wear chemical resistant po...

Page 178: ...strument exhaust and laboratory exhaust system line connections Exhaust trap bottle Warning To confirm the integrity of the source exhaust system the following leak test must be performed Caution To a...

Page 179: ...ide a drain line over the barbed fitting of the drain located at the bottom of the instrument Warning The waste line and connection can be contaminated with biologically hazardous materials Always wea...

Page 180: ...nd is above the surface of the waste solvent Positioning of drain tube Warning To prevent leakage of biologically hazardous materials ensure that the drain line does not crimp or bend A crimp or bend...

Page 181: ...ect the workstation 1 Connect the monitor to the PC 2 Connect one end of the shielded network cable to the appropriate port on the rear panel of the instrument 3 Connect the other end of the shielded...

Page 182: ...anel includes two removable connectors that hold the screw terminals for I O signals These connectors are keyed so that they can receive a signal cable inserted only one way Warning To avoid electric...

Page 183: ...3 4 5 6 7 8 9 10 Analog Out Analog Out Ground Not Used Not Used Stop Flow Out Stop Flow Out Ground Switch 2 Out Switch 2 Out Connector I 1 2 3 4 5 6 7 8 9 10 Inject Start In Inject Start In Ground Ev...

Page 184: ...al connections Description Analog Out Used for analog chart output functionality The output voltage range is 0 to 2 V The resolution of the voltage output is 12 bits Stop Flow Out Used to stop the sol...

Page 185: ...egative leads of the signal cable to the connector 3 Slide the clamp with the bend facing down into the protective shield 4 Insert the clamp and shield with the bend facing down into the connection co...

Page 186: ...the signal cable into the connection cover and position the clamp over the cable leads Tighten the clamp into place with the second self tapping screw 6 Place the second connection cover over the fir...

Page 187: ...d of the power cord to the receptacle on the rear panel of the instrument 2 Connect the male end of the instrument power cord to the 200 to 240 V AC wall outlet prepared as described in the instrument...

Page 188: ...B External Connections 188 December 6 2012 71500126802IVD Rev C...

Page 189: ...mpliant Solvents Warning To confirm the integrity of the source exhaust system you must address any safety issues raised by the contents of this Appendix Contents Topic Page Preventing contamination 1...

Page 190: ...used with these items See page 191 for details about the most common ingredients used to prepare mobile phases Items exposed to solvent Item Material Autotune reservoirs High density polyethylene Cor...

Page 191: ...ile Formic acid 0 1 Acetic acid 0 1 Trifluoroacetic acid 0 1 Ammonium acetate 10 mM Ammonium formate 10 mM These solvents are not expected to cause any problems with the materials identified in the ta...

Page 192: ...C Materials of Construction and Compliant Solvents 192 December 6 2012 71500126802IVD Rev C...

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Summary of Contents for TQ

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WATERS CORPORATION TQ, Operator'S Manual, Page: 13 / 192

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