Thermo Scientific TSQ 8000, User Manual

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4
Creating a Method
Creating a MS Instrument Method
Thermo Scientific TSQ 8000 Evo User Guide 69
Figure 68. Acquisition-Timed Scan Settings
i. The Window Optimization pane allows access to the window optimizer
settings. When the optimize button is checked, acquisition windows will be set
automatically based on the acquisition window and dwell time targets set in this
pane. For complex SRM or SIM methods, such as a method containing more
than three hundred SRM transitions, this option will help ensure a method is
created that can achieve the requested scans per peak.
Algorithm Details: If the Optimize checkbox is checked, the SRM acquisition
windows in the method are set to the Desired Window unless the Desired Min
Dwell Time cannot be met with the number of Requested Scans Per Peak . If
this occurs, then the acquisition windows are reduced until either the Desired
Min Dwell Time is met, or the Minimum Window is reached. If the Minimum
Window is reached first, the Minimum Dwell Time is reduced until the
Requested Scans Per Peak is achievable. If the absolute minimum dwell time on
the instrument, which is 0.5 ms, is reached before the requested points across the
peak are achieved, the
Minimum Window is lowered until the Desired Scans
Per Peak criteria are met or until the absolute allowed minimum window on the
instrument is reached, which is 0.24 min. In this very rare case you must reduce
the number of
Desired Scans Per Peak , increase the Min Baseline Peak Width ,
or reduce the number of transitions contained in your method before you will be
allowed to save your method (see
Chapter 7, “Troubleshooting.” ).
Scan Settings