Thermo Scientific TSQ 8000, User Manual

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5
Optimizing Your Method
Modifying an Automatic Tune
108 TSQ 8000 Evo User Guide Thermo Scientific
Figure 106. Tuning the Q3 Lenses
a. Q3 Voltage —Controls the voltage that pulls the ions into the quadrupole. The
voltage applied to this component will have a very strong effect on the energy of the
ion beam, which will have a strong effect on the resolution and the intensity. The
lower the voltage, the better the resolution. However, higher voltages will pull more
ions into the quads, which leads to better signal. This field is typically set between
0 and –5 V.
b. Resolution —Adjusts the ratio of the quadrupole DC and RF voltages to create the
resolution required for your analysis. You can set the desired peak width at a given
mass and whether you measure the width at 10% or 50% of the peak height. Because
there is no static DC voltage involved, the start, stop, and step values are not used.
c. Mass —Click in this box to select the ion to be used for tuning.
d. Start —Enter the starting voltage for the tune. The start voltage must always be less
than the stop voltage. For example, –35 is smaller than 0.
e. Stop —Enter the final voltage for the tune.
f. Step —Enter the increment for the tuning range. For example, if you tune from 0-50
in increments of 10 V, then you would set the Step field to 10.
g. Max Width —Enter the maximum allowable width of the ion during the tune.