Thermo Scientific TSQ 8000, User Manual

Page: 82 / 150

4
Creating a Method
Creating a MS Instrument Method
60 TSQ 8000 Evo User Guide Thermo Scientific
Figure 57. Determining Groups Total Scan Time
iii. Use the Chrom Filter On column to enable the chromatographic filter. This
filter smooths spectral data as it is acquired, which may increase the
signal-to-noise ratio by a factor of two or more. The chromatographic filter is
most useful when at least four full scans are acquired across a GC peak. This
setting is typically left on.
iv. Use the Chrom Filter Peak Width column to set the peak width to match the
width of the GC peak (in seconds). If the peak width is set too large, signal
intensity may be reduced. The default value is 1 s.
v. Use the Filament On column to turn the filament on and off in the selected
segment. Turning off the filament increases the lifetime of the filament and keeps
the ion source clean longer. However, no data is collected. Use this column if you
had analytes eluting before the solvent peak. You can create a segment to turn off
the filament during the solvent peak to preserve the filament.
vi. Use the Emission Current column to set the emission current used during the
acquisition. For optimal analytical performance and stability, use the emission
current at which the system was tuned. However, if you want to use a different
emission current, clear the
Use Tune File Emission Current check box and enter
a value in the Emission Current (μA ) column. A high emission current will lead
to the production of more ions, but the interaction of too many ions in the
source can cause a degradation in the resolution and signal. The margin of error
is ± 0.5 μA.
vii. Use the Use Last Tuned Detector Gain column to indicate that you want to use
the detector gain set in Auto Tune. If you do not need to use the gain set in Auto
Tune or saved to the instrument in Manual Tune, then you can set the gain
manually. Higher gains give larger signals, but may shorten the lifetime of the
detector when concentrated samples are detected.