Thermo Scientific TSQ 8000, User Manual

Page: 131 / 150

5
Optimizing Your Method
Modifying an Automatic Tune
Thermo Scientific TSQ 8000 Evo User Guide 109
h. Measure at % —Click in this box to select the location on the peak at which you
want to measure the maximum width.
• 10 —Measures the width at 10% of the peak height.
• 50 —Measures the width at 50% of the peak height.
i. Threshold —Enter the change in intensity that has to occur when the tune selects a
new voltage. For example, if you set this field to 1.1, the tune will not select a new
voltage for that component, unless the intensity is 110% of the old intensity. If you
set this field to 1, anytime the new voltage has an intensity larger than the old
intensity, the tune will select the new intensity.
j. On Error —Click in this box to select how to handle an error in the tune.
• Fail —Stops the tune when an error occurs.
• Continue —Allows the tune to continue on to the next device when an error
occurs.
k. Entrance lens offset and Exit lens offset —Changing these voltages affects ion
acceleration. The default levels will give the best performance; changing the voltages
will affect peak resolution.
l. MS/MS Voltages —These overwrite the Q3 voltage tune values when the instrument
is in SRM mode. These values are not tuned and are added to the collision energy
used in the SRM scan.
4. Click the Q3 Resolution tab to configure the resolution of your tune. You must select the
Show Advanced Settings check box to access this tab. Select this option to tune the
resolution of Quadrupole 3 by itself. This resolution will tune the system at 100 and
1000 amu/s scan rates. You may also tune at a higher scan rate. You can tune the
resolution during a lens tune or you can tune the resolution by itself. See
Figure 107 .