Thermo Scientific TSQ 8000, User Manual

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Creating a Method
Creating a MS Instrument Method
50 TSQ 8000 Evo User Guide Thermo Scientific
a. Number of Solvents—Choose Single or Multiple depending on the number of
solvents you will use for your method. You may choose up to four solvents: A, B, C,
or D.
b. Wash Station—Choose Standard Wash Station or Large Wash Station. Refer to the
autosampler documentation for more information about the wash stations.
c. Pre-injection—Use the Solvent and Cycles fields to set the number of solvent purges
that will occur before the autosampler touches your sample. Always include some
sample rinses, either before or after injection, to make sure you do not have sample
carryover from one injection to the next. Configure the settings so that the syringe is
purged with the same solvent that was used in location A, B, C, or D or with solvents
A and B or C and D. Typically, there is 0 or 1 cycles of pre-injection purges.
d. Rinse—Use the Rinses list to select the number of times the syringe is rinsed with
your sample before each injection. Rinses help ensure the sample being injected is not
diluted by the residual rinse solvents. By purging the syringe with your sample before
injection, the dilution is minimal. The standard setting is between 1 and 3 rinses. If
you have a very limited amount of sample, however, you may want to set this field to
0 to conserve the sample.
e. Post-injection—Use the Solvent and Cycles fields to set the number of solvent purges
that will occur after the autosampler touches your sample. You should always have
some sample rinses, either before or after injection, to make sure you do not have
sample carryover from one injection to the next. You can have the syringe purged
with the same solvent that was used in location A, B, C, or D or with solvents A and
B or C and D. Typically, there are 1 to 5 cycles of post-injection purges.
Creating a MS Instrument Method
Use the Method Editor to create an acquisition or maintenance method for the
TSQ 8000 Evo mass spectrometer.
 To create a method for the TSQ 8000 Evo mass spectrometer
1. Click the TSQ Series icon in the left pane to open the method view for this instrument.
2. From the Method Type list, select Acquisition-General or Acquisition-Timed. See
Figure 50 . An Acquisition method is used to collect data.