Thermo Scientific TSQ 8000, User Manual

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Thermo Scientific TSQ 8000 Evo User Guide 15
2
Changing the Column
The TSQ 8000 Evo GC-MS/MS system ships with a factory-tested 15 m × 0.25 mm ID
TR-SQC column, which the field service engineer uses to qualify the instrument. However,
once it gets dirty, you cannot purchase a new one, so you must install a column that
accommodates the type and quantity of samples that you will be running. For best results,
choose a column that will give you the best possible resolution, analysis speed, and
quantitation.
Determining the Column Type
When determining the type of column for your particular needs, here are a few things to
consider:
• Column Material —Columns made out of fused silica are economical and widely used.
Columns made out of this material have a wide range of stationary phases and are
available in many sizes that can be used with a mass spectrometer.
Large diameter columns made of steel are widely used in process gas analysis, but they are
not typically used on mass spectrometers. There are also metal-clad, fused silica columns,
which have the advantages of fused silica, but the metal makes them resistant to breakage.
These columns are less common and more expensive.
• Stationary Phase —The stationary phase is the most important consideration when
selecting a column. The interaction between the stationary phase and the analyte
determines how well the analytes separate from each other (resolution) and also affects
how quickly the separation occurs (analysis time). Choose a stationary phase that is
compatible with the nature of your analytes and the maximum GC oven temperature that
you will use.
Contents
• Determining the Column Type
• Replacing the Factory Installed Column
• Connecting the Column to the Transfer Line