Thermo Scientific TSQ 8000, User Manual

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2
Changing the Column
Connecting the Column to the Transfer Line
Thermo Scientific TSQ 8000 Evo User Guide 21
b. Run the slow temperature program that is recommended by the manufacturer. A
typical program would hold the column at 40 °C (104 °F) for 15 minutes, and then
ramp at 10 °C/min (50 °F/min) up to 10–20 °C below the maximum allowed
column temperature. Hold the column at this temperature for two hours.
Connecting the Column to the Transfer Line
When connecting the column to the transfer line, you may use either the spring loaded
transfer line nut with the graphite Vespel™ ferrule or the regular transfer line nut
.
 To connect the column using the spring loaded transfer line nut
1. Lower the oven temperature and allow it to cool.
2. If the TSQ 8000 Evo system is running, shut down and vent it. See the instrument’s
hardware manual for instructions.
3. Unwind about one turn of the column from the column outlet end.
4. Wipe approximately 300 mm (12 in.) of the column with a tissue soaked in methanol.
5. Choose an appropriate ferrule for the outer diameter of your column.
6. Insert the column through the spring loaded transfer line nut and ferrule, entering
through the tapered end of the ferrule.
7. Wipe the column again with a tissue soaked in methanol.
CAUTION INSTRUMENT DAMAGE: Never exceed the column manufacturer’s
maximum operating temperature.
Note For best results, we recommend you use the spring loaded transfer line nut.
Note If you use a graphite Vespel ferrule with your column, Thermo Fisher Scientific
recommends using the spring loaded transfer line nut with it. See the spare parts guide for
ordering information.
CAUTION BURN HAZARD: The injector, detectors, oven, and transfer line may be hot.
Allow them to cool before touching them.
Note Wear clean, lint- and powder-free gloves when you handle the column and
transfer line ferrule.