4
Tuning with Your Analyte in LC/ESI/MS Mode
Optimizing the MS Detector Tune Automatically with Your Analyte
Thermo Electron Corporation
LTQ XL Getting Started
77
Optimizing the MS
Detector Tune
Automatically with
Your Analyte
Optimize the MS detector tune automatically to maximize the ion
transmission of reserpine (or your analyte of interest) for a high-flow
experiment. Thermo Electron recommends that you begin optimizing after
you have successfully passed an automatic tuning procedure and an
automatic calibration procedure with the calibration solution infused at
5
μ
L/min.
The following procedure describes how to optimize the MS detector Tune
Method with the reserpine
m/z
609.2 peak at an LC flow rate of
400
μ
L/min. You can also carry out this procedure with your analyte of
interest and at your particular LC flow rate. (Refer to
for guidelines
about setting flow rates and temperatures.)
Optimize the MS detector Tune Method
1. On the Control/Scan Mode toolbar, click the
Tune
button to display
the Tune dialog box.
2. If necessary, click the
Automatic
tab to display the Automatic tuning
page. See
3. In the What to Optimize On group box, select the Mass option button
to make active the Mass spin box.
4. In the Mass spin box, enter
609.2
(or the appropriate mass of your
analyte of interest) to specify that the LTQ XL MS detector is to use the
peak at
m/z
609.2 (or the appropriate mass of your analyte of interest) to
optimize your tune.
5. Ensure that the Divert/Inject valve is in the Detector position, as
follows:
a. Click the
Divert/Inject
button to open the Divert/Inject Valve
dialog box. See
.
b. Click the
Detector
option button.
c. Click
Close
.
6. Start the automatic tuning procedure from the Tune dialog box, as
follows:
a. Click
Start
. A message box displays the following message:
Please ensure that the 500 microliter syringe is full.
