A
Reserpine
Thermo Electron Corporation
LTQ XL Getting Started
145
9. Mix this solution (10 pg/
μ
L=16.4 fmol/
μ
L) thoroughly. This is the final
dilution for the Reserpine Tuning Solution. If you are stopping at this
dilution, label it as
Reserpine Tuning Solution (10 pg/
μ
L)
. It best to use
this solution immediately after it is made. Keep it in a light resistant
container in a refrigerator if it must be stored. If you are making a
Reserpine APCI Sample Solution, continue with
.
10. Transfer 100
μ
L of the 10 pg/
μ
L solution into a clean polypropylene
tube.
11. Add 900
μ
L of 1% acetic acid in 50:50 methanol:water to the tube.
12. Mix this solution (1 pg/
μ
L=1.64 fmol/
μ
L) thoroughly.
13. Transfer 100
μ
L of the 1 pg/
μ
L solution into a clean polypropylene
tube.
14. Add 300
μ
L of 1% acetic acid in 50:50 methanol:water to the tube.
15. Mix this solution (250 fg/
μ
L=411 amol/
μ
L) thoroughly.
16. Label the tube
Reserpine APCI Sample Solution (250 fg/
μ
L)
. It is best to
use this solution immediately after it is made. Keep it in a light-resistant
container in a refrigerator if it must be stored.
