Thermo Scientific Dionex ICS-1100, Operator'S Manual

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Doc. 065289-03 10/12
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E • Glossary
Analytical Column
Synonymous with Separator Column .
Band Spreading
The broadening of the sample band as it travels through the column. Band
spreading can also occur in the injection valve, detector cell, and interconnecting
tubing.
Calibration Curve
A graph showing detector response in peak height or area versus analyte
concentration.
Capacity Factor (k´)
The number of column volumes of eluent, pumped through the column, required
to elute an analyte. Capacity factor is a dimensionless measure of retention which
is independent of column length or eluent flow rate. It is calculated as follows:
Where: t
r
= retention time
t
o
= retention time of unretained solute (column void volume)
k

t
r
t
o
–
t
o
-------------- =