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PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION 

 

3, Hagavish st. Israel 58817  Tel: 972 3 5595252, Fax: 972 3 5594529   [email protected] 

 

MRC.VER.01-5.12 

 

Operation Manual for 

FLUORESCENT SPECTROPHOTOMETER

 

SPECTRO-96

 

 

Summary of Contents for SPECTRO-96

Page 1: ...ASE READ THIS MANUAL CAREFULLY BEFORE OPERATION 3 Hagavish st Israel 58817 Tel 972 3 5595252 Fax 972 3 5594529 mrc mrclab com MRC VER 01 5 12 Operation Manual for FLUORESCENT SPECTROPHOTOMETER SPECTRO...

Page 2: ...ument should have a basic knowledge of using optical instruments and a basic knowledge of molecular fluorescence spectroscopy The user whose instrument is equipped with a computer also should have a c...

Page 3: ...tially hazard to the electrical appliances through the operation and indicates that a qualified professional man is needed to deal with such a hazard according to the corresponding procedures This lab...

Page 4: ...as possible 5 When the instrument is working the surface temperature of the heat dissipation opening at the top of the left back side of the instrument xenon lamp position becomes very high Be sure no...

Page 5: ...on of Startup 17 2 2 1 Startup of the Instrument 17 2 2 2 Confirmation of Heat Dissipation Fan Running 17 2 2 3 Initialization 17 Table 1 2 Fluorospectrophotometer Initialization List 18 2 3 Measureme...

Page 6: ...Time Scan 37 6 2 3 The Interface of Quantitative Analysis 38 7 Operation of Wavelength Scan Mode Connected with a Computer 39 7 1 The Interface of Wavelength Scan Mode 39 7 1 1 The Functions of Short...

Page 7: ...3 9 2 3 Zero point Adjustment 53 9 2 4 Standard Sample Setting 54 9 2 5 Measurement of Standard Samples 54 9 2 6 Method of Undetermined Coefficients 55 9 2 7 Measurement of Samples 55 9 2 8 Set Sample...

Page 8: ...e Complete Instrument Operating Panel Fig 1 1 SPECTRO 96 Fluorospectrophotometer Main Unit Heat Dissipation Opening at the Top of the Instrument Heat Dissipation Fan Power Line Lid of Sample Compartme...

Page 9: ...it a cell holder used to hold a sample cell Power Switch used to turn on off the power source of the instrument Fuse Holder used to mount a fuse Power Socket used to interconnect a feed cable Xenon La...

Page 10: ...9 Main Components 1 Xenon Lamp Fig 1 3 Xenon Lamp and Lamp Holder Line Xenon Lamp The lightening part Line Lens INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER...

Page 11: ...ilter center wavelength 365 nm 2 Sample Compartment Fig 1 4 Sample Compartment and Quartz Sample Cell 10 mm 3 Filter Filter Position Sample Cell Position INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTR...

Page 12: ...ectrop hotometer Main Unit 1 Mode key 2 Print key 3 Adj 0 key 4 Normalize key 5 Display Window 2 5 4 3 1 1 2 Operating Panel of the Instrument 1 Appearance and Performances of the Instrument INSTRUCTI...

Page 13: ...s of the Instrument Xenon Lamp Power Source of Xenon Lamp Excitation Filter Sample Cell Emission Monochromatoraton Photomultiplier Preamplifier Monolithic Processor Serial Interface USB Communication...

Page 14: ...13 Optical System Fig 1 8 Optical System Arrangement of SPECTRO 96 Fluorospectrophotometer INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER...

Page 15: ...1 Measuring with a single instrument By using the functions of the keys and the functional indicator lights on the instrument panel read the measured data on the display window 2 Measuring with an on...

Page 16: ...racy within 1nm Wavelength repetition 0 5nm Emission spectrum bandwidth 10nm S N ratio S N 150 Using 1cm quartz sample cell measure the signal noise ratio of Raman spectrum of water Check utmost 1 10...

Page 17: ...lue use Mode key to cancel current value and return the set value 2 Print Key The Print key can print the current value if a dedicated serial interface printer is installed in Fluorescence Value Mode...

Page 18: ...e unnecessary number of turning on off the xenon lamp should be cut down as far as possible and the xenon lamp should avoid being turning on off repeatedly under a high voltage 2 2 2 Confirmation of H...

Page 19: ...ed Photomultiplier tube error PMT Module error Self checking Motor Module Motor Module initialized Motor error Motor driver board error Self correcting Wavelength correcting The value from bottom to t...

Page 20: ...ethods that is multipoint method of standard sample and single point method of standard sample The two operating methods are as follows 1 Measuring procedures by Multipoint Method of Standard Sample P...

Page 21: ...on of maximal concentration into sample cell Then press Mode to enter PMT level adjust Current PMT Level shows the current level The higher the level is the more sensitive it will be Use Adj 0 key or...

Page 22: ...ument Turn off the power source of the Fluorospect rophotometer main unit first and then the power source of the xenon lamp The order is reverse to that of startup If in on line mode close the softwar...

Page 23: ...violet radiation should not be contained in the atmosphere 3 1 2 Working Table The instrument should be placed on a sturdy and level table or the like avoiding strong vibration direct sun light and d...

Page 24: ...g marks of impact on the packing please contact the transport insurance department 3 2 2 Unpacking for Counting Unpack at the position of adhesive seal tape Take out the Fluorospectrophotometer main u...

Page 25: ...ference filter 25nm Quartz sample cell 10mm Glass sample cell 10mm Fuse 2A 5A USB wire Spare parts Purchased by choice Power line Microcomputer main unit Display device Dedicated serial interface prin...

Page 26: ...ument should accord with that of the local 3 3 3 Connecting the Power Cord Place the Fluorospectrophotometer main unit and the computer or the printer on a sturdy working table and they should be 10cm...

Page 27: ...d 200 Find the wavelength value max of the maximum fluorescent intensity at about 415nm For more information of adjustment please turn to 2 3 2 Then observe for 2 minute at the wavelength max Record t...

Page 28: ...mputer Finish the measurement for Raman Spectral Peak of water by using wavelength scan and time scan in the computer software The theory is the same as Section 3 4 1 Part 1 The use of software please...

Page 29: ...do such work The concrete method and the correcting procedures will be briefed through training The wavelengths of spectral lines of a mercury arc lamp are listed in the Table 1 1 and the spectrogram...

Page 30: ...ning at the top of the instrument It is unsuitable to stack other articles nearby Note The temperature at the heat dissipation opening is very high Be sure not to touch for fear of being burnt 3 Keep...

Page 31: ...non lamp fails to be lit and a squeaking sound of high frequency or a cracking sound of sparking is continuously produced please turn off the power source of the xenon lamp immediately Several seconds...

Page 32: ...over board on the excitation part from the top left of the knurled screw 3 Take out the excitation part 4 Remove the front pressing ring of the excitation part anticlockwise 5 After that hold the both...

Page 33: ...32 Part Two Operation Instruction for the Instrument Connected with a Computer INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER...

Page 34: ...wo serial ports of the computer should run normally and no interruption conflict occurs in the system Image resolution is more than 800 600 and color enhancement is more than 16 bit 5 1 2 Software Req...

Page 35: ...e is The Software Package of Fluorospectrophotometer You may click NEXT button directly or change the item name then confirm the dialog boxes of all above steps If hoping to amend click Back button or...

Page 36: ...n line condition 3 Initialization Run Spectro96 Software The software will check the connection and starts initialization For more information please turn to 2 2 1 On completion of initialization the...

Page 37: ...36 Fig 2 1 Interface of Wavelength Scan Fig 2 2 Switchover of Working Mode PMT Storage EM Wavelength Wavelength Scan Fluor Fluor INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER...

Page 38: ...and can also be used to study the emitting behavior of fluorescent molecules in solutions The interface of wavelength scan mode is displayed as shown in Fig 2 1 6 2 2 The Interface of Time Scan Time...

Page 39: ...ncentration of the component to be determined is acquired K in the formula is a constant related to the test conditions and it can be acquired by measuring the fluorescent emission intensity of a stan...

Page 40: ...ig 2 5 Shortcuts in the Interface of Wavelength Scan of Fluorescence Spectrum 7 1 1 The Functions of Shortcuts at the Bottom of the Screen Scan is the button for starting After you click it scanning a...

Page 41: ...uantitative analysis mode Parameter button means to set scan parameters Restore Parameter button means to restore the spectrum of current channel to its original status Smooth button means to carry ou...

Page 42: ...rameter the interface of wavelength Scan Parameter will appear as shown in Fig 2 6 Fig 2 6 Interface of Wavelength Scan Parameter 1 EM Start Set the start wavelength of scan 2 EM End Set the end wavel...

Page 43: ...wavelength scan Batch will show all data after the wavelength scan 11 Slit Its value is unchangeable and it has a bandwidth of 10nm Fig 2 7 Interface of Wavelength Scan Parameter Replicate Scan Setti...

Page 44: ...tly use the mouse to drag the spectrum on the screen The method is to drag in the direction from the top left side of the spectrum to the bottom right side of the spectrum If you want to return the en...

Page 45: ...between channels Fig 2 10 Interface of Arithmetic Operation 7 Sigma After clicking this item input Lambda Start and Lambda End and then click the Sigma button You will get the integral value of fluor...

Page 46: ...he spectrum of one channel into another channel 4 Save Spectrum Save the selected spectrum and the file name onto the hard disc 5 Save Spectrum As Save the spectrum onto the hard disc After a dialog b...

Page 47: ...ange the background color of the working window 3 Set Background Change the background color of scanning window 4 Set Netware Change the net type of the scanning window 5 Export Window as Export the c...

Page 48: ...nged to the interface of time scan See Fig 2 3 The time scan interface is the same as that of wavelength scan interface except for the unit of X axis The functions of the shortcuts are also the same F...

Page 49: ...rum The software will automatically add the current time of scan after the filename 13 Auto Saved Direction Set the autosave position in your computer 8 1 3 Spectrum Processing The functions and the o...

Page 50: ...tering the interface of Quantitative Analysis Above the interface of quantitative analysis on the top of the screen the first row is menu bar The second row has 15 icons The screen shows the Current W...

Page 51: ...will show Icons are arranged in order from left to right The functions are described bellow Set QA Parameter button This feature has not been activated yet Set Working Curve Parameter button means to...

Page 52: ...Method of Undetermined Coefficients The method by which an equation of working curve is created through measuring standard samples is called Method of Undetermined Coefficients For more details refer...

Page 53: ...vious data of standard solutions so as to make a direct measurement for samples of the same kind After clicking Build Curve in Data Processing menu you may see the working curve the equation and the c...

Page 54: ...9 2 2 PMT Setting There are 17 grades 1 17 Default PMT level is 1 Generally adjust PMT level by using the highest concentration of the solution Adjust the fluorescence value to make sure it is stable...

Page 55: ...is See Fig 2 20 Fig 2 20 Dialog Box of Set Standard Samples Note The parameter setting of standard samples must follow the order of concentration from low to high The names of standard samples includi...

Page 56: ...urve is created through measurement of standard samples is called Method of Undetermined Coefficients If an equation of working curve is known you may select Input Coef As for the measuring of samples...

Page 57: ...middle as shown in Fig 3 1 after inputting Fig 3 1 Screen Display after Inputting Fluorescent Intensity and Concentration 2 Enter the Chart Wizard System 1 Preview Generated Graphics Click the Chart...

Page 58: ...The Standard Curve Plotted after Data Processing With Excel 2000 Software in Measurement of Riboflavin by fluorophotometry y 437 14x 0 2762 the fitted equation correlation coefficient R2 0 9997 4 Meas...

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