Moss MH-50B, User Manual

Page: 23 / 36

MH-50B Chemistry Analyzer Operation Manual
22
GTP/ALT VELOCITY REAGENT MANUAL
1. PRINCIPLE: L-Aa-KETOGLUTARIC ACID ALT PYRUVIC ACID+ GLUTAMIC ACID,
PYRUVIC ACID + NADHLDH L- LACTIC ACID + NAD
+
When the wavelength is 340nm, test NADH absorbance decrease value every minute, calculate ALT
activity value.
2. REAGENT CONTENT, SIZE AND SPECIFICATION:
a) Single vial: 2 kit descriptions : 10ml X 10, 18ml X 10.
b) Two vials: It is consisted of RI and RIL with different kit descriptions, 10ml X 5, 18ml X 5.
3. Storage and Effective Date: 2-8°C, effective date is 12 months.
4. Reagent Mixing
Single vial: It can be used after dissolving with DI water according to the specification,
Reconstituted reagent is stable for 3 days at 2-8°C.
Two vials: Pour the RII and cushion to the RI bottle, dissolve them then use, effective date is 3
days.
5. TEST METHOD
FOR EXAMPLE:
PARA
MET
ER
SIZE
WAVE
LENG
TH
nm METHO
D TEM
P
°C DELAY
TIME TEST
TIME REACTI
ON
DIRECTI
ON REAGENT
VOL.
Ul. SAMPL
E
VOL.
Ul. FAC
TOR
ISP-I II 340 Kin 37/30 30 40 - 500 50 -176
8
Beckma
n700 340 Kin 37/30 30 40 - 500 50 -241
2
Attention: Icterus patient’s best ratio of serum to reagent is 1:20, factor is -3376.
Calculate Formula: u/l=
△
A/min X (10
3
X Vr) / (6.22 X Vs)
△
A/min: Change value of absorbance every minute Vr: Total Vol. for reaction liquor
Vs: Sample Vol.
6. Normal value: 37°C: 8-36 u/L 30°C: 4-28 u/L
7. Reagent characters and technical specifications:
1) Characters: This reagent is packed with screw-mouth plastic bottle, it has long effective date. The
double-bottle reagent is made to cushion with high purified DI water, it solves the unpurified DI water
of user and pollution of microorganism. The cushion is installed in R II bottles with certain quantity
according to the specification.
2) Technical specifications:
3) The ratio of this reagent to Beckman reagent is
γ : 0.994, in one group CV: 2.8%
Between two groups 3.9% Linearity ≤ 600u/L
Special note: Please write down and save the tested item parameters to avoid the system’s internal
memory to be disturbed by environment.