MH-50B Chemistry Analyzer Operation Manual
21
6
．
TEST METHOD AND PROCEDURE:
6.1 HANDWORK:
SAMPLE TUBE
STANDARD TUBE REAGENT BLANK TUBE
SERUM 50
-
-
STANDARD
LIQUOR ul
- 50
-
GPT BASE
METERIAL
LIQUOR ul
250 250
250
Mixing, 37°C for 30 minutes or 50°C for 15 minutes, then add GPT developer 250ul into every tube,
37°C for 15 minutes or 50°C for 6 minutes, get all tubes , add diluted NaOH 2.5ml, lay them aside for
3 minutes, test wavelength 510nm. Adjust REAGENT BLANK to 0, test absorbance of sample tube
and standard tube.
SAMPLE TUBE GPT u/L =A sample/A standard X 100
6.2 SEMI-AUTO ANALYZER TEST BY ENDPOINT:
Operation procedure is the same to HANDWORK.
Adjust wavelength: 510nm, Adjust REAGENT BLANK to 0.
Input standard 100 , Absorb standard liquor(Attention: Electric knob instrument sample the
standard liquor then modify the standard to100). It will show the test result after absorbing the sample
liquor one-by-one.
7
．
NORMAL VALUE:8-50u/L
8
．
TECHNICAL INDEX AND NOTICE EVENTS:
BLANK ABSORBANCE A
510nm
<0.3, CV: 4.7%, LINEARITY
≤
300u
Attention: Keep the standard liquor airproof without pollution. Write down the factor value testing
for first
time for the instrument which has factor function, input factor, test sample tube’s consistency without
doing standard tube
(2). Kinetic
For example: GTP/ALT velocity reagent manual, input parameters in turn as follow:
TEST CODE: 1 NAME: GTP/ALT REAGENT SUPPLIER: HUILI
COEFFICIENT: 1746 UNIT: u/L METHOD: KINEMIC
TEMP CONTROL: 37°C DECIMAL: 0 WAVELENGTH: 340
REAGENT BLANK: N SAMPLE BLANK: N HIGH REF.: 36
LOW REF.: 8 LINEARITY RANGE: <500 DELAY TIME: 30S
INTERVAL TIME: 40S SAMPLE TIME: 300 SAMPLE POSITIONS: 2
STD.: 0
The circles in reagent manual shows how to confirm the parameters. REAGENT BLANK,SAMPLE
BLANK, SAMPLE TIME, SAMPLE POSITION, STD. have not explained in reagent manual, please
explain the setting according to the manual.
