ibidi Pump System
Possible Cause
Solution
Blockage in the air pressure tubing lead-
ing from the pump to the drying bottle or
from the drying bottle to the incubator.
Make sure that the air tubes are not being squeezed
and that there is no blockage inside the tubing. Es-
pecially check the setup of the drying bottle. If the
tubing looks fine, remove it from the rear port. If the
issue persists, contact the
for
assistance.
10.7
Flow Rate is Too Low or Absent
Issue:
The pump applies the correct pressure to the Fluidic Unit (target pressure and current pres-
sure are matching), but the flow rate is much lower than predicted by the software or there is no flow
visible at all in the Perfusion Set.
Possible Cause
Solution
There is a blockage in the tubing leading
from the pump front port to the Fluidic
Unit.
Check for any blockages and clean tubing if possible.
If not, contact
for assistance.
Clogged filters restrict the air pas-
sage. When filters come in contact with
medium, the air flow is decreased or
ceases.
Filters that are contaminated with medium must be re-
placed (Section
When applying low pressure, even a sin-
gle air bubble in the tubing can decrease
or even stop the flow.
Check the tubing for air bubbles. If necessary, discon-
nect the slide and remove the air bubbles from the tub-
ing (see Section
). Then re-connect your slide and
continue with your experiment.
None of the above.
Contact the
for assistance.
10.8
Flow Rate is too High
Issue:
The manually measured flow rate (see Section
) is much higher than indicated by the
software.
Possible Cause
Solution
The tubing was not inserted correctly into
the pinch valve (valve 2) and produced
a “by-pass” that leads directly from the
source reservoir to the sink reservoir,
leading to a high flow rate.
Check that the Perfusion Set is correctly mounted by
performing the pinch test (Section
Wrong calibration factor.
Check the flow rate again with a stop watch and adjust
the calibration factor in the software. This procedure
is detailed in Section
Instruction Manual
77
Version 2.6 (May 12, 2022)
