ibidi Pump System
10.9
Total Medium Volume in the Perfusion Set is Decreasing
Issue:
Long-term experiments could result in medium evaporation. The amount of volume loss per
day depends on the humidity in the incubator and the volume flow rate. Do not exceed a volume
loss of more than 10% of the total volume.
Possible Cause
Solution
The air stream in the reservoirs results in
a slight evaporation of medium, which is
normal.
Depending on the requirements of the cells, exchange
the medium after a few days or refill the evaporated
amount of volume with sterile water.
The humidity in the incubator is not high
enough.
Check the humidity of the incubator with a hygrom-
eter. Small and low priced hygrometers are available
in electronic gear shops. The minimum recommended
humidity is 80% rel. humidity.
10.10
Flow Direction in the Channel is Changing
Issue:
The flow direction in the slide changes even though it is set to unidirectional flow.
Possible Cause
Solution
Perfusion Set was not mounted correctly.
Check that the tube is inserted properly.
Pinch valve (V2) is not switching.
Check the functionality of the pinch valve:
when switching the valve via the PumpControl
software, the movement of the pinching bar can
be seen from the side (see Figure
). If it does
not move, contact the
for
assistance.
10.11
Pressure Lost Error
Issue:
The program shows the ”Pressure Lost” error message. The pump emits loud beeping.
Possible Cause
Solution
The pressure set in the pump cannot be
maintained.
Check for possible leakages along the air pressure tub-
ing between the pump controller and the Fluidic Unit,
like loosened connections or holes in the air pressure
tubing.
The pressure set in the pump is perma-
nently exceeded.
Check for possible blockages along the air pressure
tubing between the pump controller and the Fluidic
Unit (e.g., in the connection parts of the Drying Bot-
tle). Remove the blockage.
The pressure is set to ”zero”.
A pressure of ”zero” also causes an ”Pressure Lost”
error message. Set the pressure to a value within the
specifications (5–95 mbar).
The power supply has an unstable con-
nection.
Check the correct fit of the plug and fasten if necessary.
Version 2.6 (May 12, 2022)
78
Instruction Manual
