ibidi Pump System
2
Intended Use of the ibidi Pump System
The ibidi Pump and Fluidic Unit(s) create unidirectional long-term flow of medium within a channel
slide (e.g.,
). This constant flow mimics physiological conditions for cell types,
like endothelial cells of the blood or lymphatic system, which experience constant flow
in vivo
.
The mechanical force generated by fluid flow on cells is called (wall) shear stress (
dyn
cm
2
or
Pa
). Under
physiological conditions, a laminar flow leads to a shear stress on the cell layer on the vessel wall
which is proportional to the velocity of the fluid. The shear stress varies among different tissues and
organisms.
The ibidi Pump System offers the following advantage for cell culture under flow:
• Covering of the whole physiological range of shear stresses.
• Precise control of flow conditions with the PumpControl software.
• Defined shear stress calculation in the ibidi Channel Slides.
• Unidirectional flow for long-term studies (up to weeks).
• Oscillatory and pulsatile flow to mimic turbulent flow situations and pulsatile blood flow.
• Easy access to the culture vessel for imaging on the microscope during incubation.
• Experimental setup under sterile conditions possible.
• Minimization of the medium consumption with a circulating medium flow.
• Minimization of mechanical stress on suspended cells to avoid destruction and non-specific
cell-activation.
The ibidi Pump System is for research use only! It is not intended for use in diagnostic procedures.
Instruction Manual
15
Version 2.6 (May 12, 2022)
