ibidi Pump System
7.4
Pulsatile Flow Experiments
In order to create pulsatile flow two Fluidic Units are required. The first Fluidic Unit will create a
unidirectional flow which the second Fluidic Unit will periodically interrupt to create pulsation.
Follow the steps below for correct installation:
1. Connect the first Fluidic Unit to Port 1 and the second to Port 2 on the ibidi Pump.
2. Connect the air pressure tubing to the first Fluidic Unit only.
3. Mount the Perfusion Set on the first Fluidic Unit.
4. Set up a continuous and unidirectional flow with the desired flow rate and the resulting shear
stress with the help of the PumpControl software.
5. Route the tubing of the Perfusion Set which is coming out of the first Fluidic Unit through the
pinch valve of the second Fluidic Unit. Make sure that you use either the two front or the two
rear openings of the pinch valve for that. Do not intermix front and rear openings. Compare
your setup with Figure
6. Run the second Fluidic Unit in the PumpControl software as an oscillating Unit. This way you
can select the switching time in the range of 0.2 s – 5 s which results in pulsation periods of
0.4–10 s. This in turn corresponds to frequencies of 2.5–0.1 Hz. Compare with Figure
Figure 45: Set-up for pulsatile flow. The continuous flow created by Fluidic Unit 1 is periodically
pinched off by Fluidic Unit 2.
Version 2.6 (May 12, 2022)
58
Instruction Manual
