ibidi Pump System
5. Conduct at least four time measurements and calculate the mean value. If necessary, perform
more measurements to optimize your measurement error.
6. To calculate the flow rate [ml/min], insert the time that was measured (mean value in seconds)
in the formula below.
Φ
ml
min
=
2
[
ml
]
·
60
s
min
t
[
s
]
Example experiment:
The measurement is explained with the following example:
Parameter
Example setup
Slide
µ-Slide I
0.6
Luer
Perfusion Set
RED (15 cm, ID 1.6 mm)
To make sure the flow rate is exactly 23.8 ml/min, measure it manually as follows:
1. The shear stress required is defined as 10 dyn/cm
2
.
2. Open the PumpControl software. In the “Fluidic Unit Setup” tab, choose the RED Perfusion
Set and “µ-Slide I
0.6
Luer” from the drop-down menu. Insert a viscosity of 0.007 dyn*s/cm
2
and for the calibration factor to be set at ”1”, then click the “Apply Settings” button.
3. Proceed to the “Flow Parameters” tab. Enter the shear stress (10 dyn/cm
2
) in the respective
box. The flow rate (23.8 ml/min) and pressure (33.1 mbar) are now calculated automatically.
4. Set up the perfusion experiment with the Perfusion Set RED and medium inside a cell culture
incubator (5% CO
2
, 37°C).
Parameter
Example setup
Shear stress required
10 dyn/cm
2
Flow rate required to obtain 10 dyn/cm
2
23.8 ml/min
Calibration factor (default)
1.0
Viscosity of medium
0.007 dyn*s/cm
2
1. Connect a µ-Slide I
0.6
Luer (Section
2. Run the program in the manual control mode, applying 33.1 mbar and measure the time for 2
ml between the 6 ml and the 4 ml mark. Take care to switch the valve before the reservoirs run
dry.
3. The mean value of the measurements is 5.7 seconds. This means the actual flow rate is 21.05
ml/min versus 23.8 ml/min as predicted in the software.
Version 2.6 (May 12, 2022)
46
Instruction Manual
