ibidi ibidi Pump System, Instruction Manual

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ibidi Pump System
1 2
A1 A2
A
B1
B
B2
Figure 41: Preparation and parts to connect the Air Pressure Splitter Set
for two Fluidic Units to the primary (1) and secondary (2) Fluidic Unit.
Follow the steps below for correct installation.
1. Remove the short tubing that standardly connects the valve block (V1) to the tubing leading to
the Perfusion Set. Keep them in a safe place to re-install them for use in unidirectional mode
(Figure
41 ).
2. Connect both Air Pressure Splitters to the respective port on the primary Fluidic Unit’s valve
block (V1). The black-marked tubing goes to the left side (marked A, Figure 42 -2) and the
yellow-marked tubing goes to the right side (marked B, Figure 42 -2).
3. Connect the remaining splitter ends (A1/A2, B1/B2) to the respective connectors at the back of
both Fluidic Units, leading to the Perfusion Sets as shown in Figure 42 -1.
4. Connect the air pressure tubing (2 m) of the pump to the air inlet of the primary Fluidic Unit.
The air inlet of the secondary Fluidic Unit remains unused (Figure 42 -3).
5. Connect the pump and the two Fluidic Units with the Fluidic Unit cables. In this example, “Port
1” on the Pump Controller for the primary Fluidic Unit and “Port 2” on the Pump Controller
for the secondary Unit are used (Figure
42 -3).
6. Mount the Perfusion Sets onto both Fluidic Units as for the standard setup (Section 6.2 ) and fill
with medium (Section 6.3 ).
7. Remove air bubbles from the tubing (Section 6.4 ) and perform the pinch test for each Fluidic
Unit (Section 6.5 ).
8. Calibrate the flow rate separately as described in Section 7.1.2 , both with unidirectional flow.
For this, completely pinch off the tubing of one Perfusion Set to stop the flow and to be able to
calibrate the other one separately.
9. Equilibrate the liquid levels of each Fluidic Unit before starting an experiment. Because the
primary and secondary Fluidic Unit(s) are connected to the same air pressure, equilibration of
the liquid levels must be performed separately. To stop the liquid movement in the reservoirs
of the other Fluidic Units, the Perfusion Sets must be clamped with a hose clip. Balance the
liquid levels evenly in the reservoirs and then move on to the next Fluidic Unit.
Version 2.6 (May 12, 2022) 54 Instruction Manual