ibidi Pump System
6.5
Pinch Test
Important!
The pinch test must be performed with each
newly inserted Perfusion Set. This test makes
sure that the tubing is inserted correctly into
the pinch valve.
1. Start a perfusion program with a clearly vis-
ible flow in the reservoirs (automated cy-
cle).
2. Pinch the tubing in the lower loop near the
Female Luer Coupler (Figure
3. Observe the movement of the liquid lev-
els in the reservoirs. While the tubing is
pinched, the liquid should stop moving.
Make sure you check both switching posi-
tions (State 1 and State 2).
→
If there is no
movement, the setup is correct, otherwise
check the mounting of the Perfusion Set.
Figure 29: Pinch test for checking the correct in-
sertion of the tubing into the valve.
Does the liquid move (switching state 1)?:
2
Yes
2
No
Does the liquid move (switching state 2)?:
2
Yes
2
No
If the answers are “No” in both cases, the insertion is correct.
If the liquid is moving in one or both of the positions, check the insertion of the tubing in the pinch
valve. Stretch the tubing and move it up and down for proper placement in the valve’s slot (Figure
). Also check for correct positioning of the tubing (Figure
). If this action does not correct the
problem, contact ibidi or your local distributor.
6.6
Calibration of the Flow Rate
The PumpControl software provides an automatic calculation of pressure, flow rate and shear stress,
once the Perfusion Set, the slide and the viscosity of the medium are set. One of the parameters
(pressure, flow rate and shear stress/shear rate) is chosen, and the other parameters are calculated
automatically, according to the relation below (calibration curve and shear stress calculation).
Instruction Manual
43
Version 2.6 (May 12, 2022)
