ibidi Pump System
6
Setting Up an Experiment With Cells
This section explains all necessary steps to setup an experiment with cells and unidirectional flow.
A more detailed cell culture protocol for performing an experiment with HUVEC under perfusion is
provided in
Application Note 13 “Endothelial Cells under Perfusion”
Note!
For first time use we recommend practicing handling with a non-sterile Perfusion Set and slide.
Each Fluidic Unit is supplied with a non-sterile Perfusion Set.
6.1
Degassing of Slides, Tubing, and Medium
To avoid air bubbles, the degassing of all plastic components and the medium is critical. Place the
following parts inside the incubator one day before starting the experiment. Sterility is maintained
as long as the packaging is not opened.
• µ-Slides (within the packaging)
• Perfusion Set(s) (within the packaging)
• Cell culture medium for cell seeding (add the volume needed to a small vessel, and loosen the
cap slightly)
This procedure is necessary because of the temperature dependency of gas solubility in water and
plastic. At higher temperatures, water and plastic can absorb less gas than at lower temperatures.
The solubility of O
2
, N
2
and CO
2
in water is shown in Figure
Figure 18: Solubility of O
2
, N
2
and CO
2
in water at 1 atm
If components have been stored at room temperature or in the refrigerator, gases dissolved in the
plastic and liquids will be released when heated up in the incubator. Gas bubbles will then appear
Instruction Manual
37
Version 2.6 (May 12, 2022)
