ibidi Pump System
When working with positive pressure, the atmosphere from the incubator is drawn in through the
pump’s rear port to ensure a saturation of 5% CO
2
. To prevent condensation inside the pump, a
drying bottle (Section
) is inserted. The setup is shown in figure
. The modified setup for
negative pressure is shown on page
5.2
Connecting the Fluidic Unit to the Pump
While the pump itself is outside the incubator, the Fluidic Unit(s) is placed inside. There are two
connections between the pump and the Fluidic Unit. The 2 m cable length is long enough to run the
cable/tubing out through the back port or the door of the incubator:
• The Fluidic Unit cable sends switching signals from the pump to the Fluidic Unit.
• The air pressure tubing provides pressurized air to the Fluidic Unit and reservoirs.
The Fluidic Unit cable’s plug is marked with a red dot that aligns with the red dot on the back of the
Fluidic Unit. The other end of the electric cable is plugged into any of the Fluidic Unit ports on the
back of the pump. The pump will automatically recognize which port is connected. If using positive
pressure, connect the Fluidic Unit directly to the pump with the 2 m air pressure tubing (Figures
and
). To ensure that the correct CO
2
amount is being drawn into the pump, connect the drying
bottle to the rear port of the pump and feed the second piece of tubing into the incubator (Section
Figure 14: Rear view of the Fluidic Unit with the air pressure tubing and the electric cable connected.
5.3
Connecting the Drying Bottle
The drying bottle protects the pump from humidity coming from the incubator. Therefore, the bottle
must be in-line with the tubing that connects the incubator and pump.
The pump pushes air out of the front port onto the reservoirs and takes in the atmosphere from
the back port. For CO
2
supply, the back port of the pump must be connected with a tubing to the
incubator atmosphere. However, as water vapor is also present in the gas from the incubator, the
drying bottle must be integrated between the incubator and pump.
Version 2.6 (May 12, 2022)
34
Instruction Manual
