ibidi Pump System
5
Basic Setup
This section details how to connect all the components for a basic setup using a 10 ml Perfusion Set.
For first-time users of the ibidi Pump System, it is recommended, to set up the system outside the
incubator and practice using deionized water.
Before setting up the experiment, make sure that you have all items listed in Section
Important!
The ibidi Pump System is intended for use in combination with a cell culture incubator with 37°C,
5% CO
2
and 80–100% humidity!
5.1
General Setup of the Components
The computer and the pump are placed next to the incubator on a stable surface (e.g., work bench).
When preparing an experiment with cells, the Fluidic Unit with the mounted Perfusion Set is placed
inside the incubator. The pump remains outside and is connected to the Fluidic Unit with the elec-
trical cable and air pressure tubing. The setup using positive pressure, which is recommended
, is
shown in Figure
Figure 13: Positive pressure system setup of one Fluidic Unit inside the incubator. The cables and
tubing are inserted through the back port of the incubator.
There are several options for leading the tubing and cable(s) inside the incubator:
• The optimal configuration is leading the tubing through an opening in the back of the incubator.
The tubing and cable can be passed through this opening, and then sealed with a suitable
modified rubber cap to prevent leakage of heat and CO
2
.
• If the incubator has no back port, lead the cable and tubing through the front door. Most
incubators have a rubber seal that is flexible enough to introduce the connections directly. The
air pressure tubing is rigid and will not be compressed by the door.
1
The setup with negative pressure is shown in figure
Instruction Manual
33
Version 2.6 (May 12, 2022)
