ibidi Pump System
Figure 32: Fill the reservoir before con-
necting the Luer adapter.
Figure 33: Connect the second Luer
adapter to the µ-Slide.
6.6.2
Flow Rate Measurement
Use a stop watch to measure and calibrate the flow rate.
Important!
The system must be calibrated with the same slide and pressure you want to use in your experi-
ment. As the flow rate is temperature dependent, perform this measurement under experimental
conditions (e.g., at 37°C in the incubator).
1. Define a shear stress you want to apply.
2. Set all experimental parameters in the automatic control panel accordingly (Perfusion Set, µ-
Slide, and required shear stress). Note down the flow rate and pressure that are calculated
automatically by the program.
3. Now go to the manual control panel and apply the indicated pressure to the Perfusion Set and
Slide.
4. Let the medium rise to
∼
7–8 ml in one reservoir and then switch the valve. Observe the liquid
level when going down and stop the time between 6 ml and 4 ml.
Figure 34: Measure the time t (in seconds) required for the medium to flow from the 6 ml mark down
to the 4 ml mark on the syringe (2 ml volume) with a stopwatch.
Instruction Manual
45
Version 2.6 (May 12, 2022)
