Hach Eclox, User Manual

Page: 68 / 124

68
Luminescent Bacteria Toxicity Test - Screening Luminescence Results
Sample: _______________________________________________________
Date: _______________ Time: _______________
Operator: ______________________________________________________
Comments:
Procedure:
1. Add 1.0 mL of
reconstitution solution to the
reagent. Swirl to mix. Wait 5
minutes.
2. Add 1.0 mL of stock
suspension to 10 mL of
Diluent. Shake to mix. Wait
15 minutes.
3. Add one spoon of solid
NaCl to 7 mL of sample.
4. Fill four test tubes with the
test suspension, 2% NaCl,
and sample in the order
shown in the table. Start the
timer after adding the first
sample volume. Set the
timer for 15 minutes.
5. Push
ON
(green button) on
the luminometer. Go to the
Screening Luminescence
Menu.
6. Measure test tube 1
(non-toxic reference).
7. Measure the sample tubes
in the order 2, 3 and then 4.
Record the results in the
table.
Order 1st 2nd 3rd %
inhib.
Rel.
units
Tube Test
susp.
(mL)
2%
NaCl
(mL)
Sample
(mL)
Sample
Conc.
1 0.2 0.8 no Non-toxic
reference
2 0.2 0.6 0.2 20%
3 0.2 0.3 0.5 50%
4 0.2 no 0.8 80%
% sample
% inhibition