89
11.10.2 Prepare a 3 dilution series (D 2 values and higher)
To make a 3 dilution series according to ISO 11348 of D 2 sample values and higher,
do this procedure.
This procedure makes dilutions ranging from undiluted to a dilution ratio of 1:2 in
row A. This corresponds to D values of 2 to 4 (
Figure 8
) in the test, as 0.5 mL of the
sample dilution is added to 0.5 mL of the test suspension during the test in Row B
and C. Adding test suspension to the sample dilutions increases the sample
dilutions in row A by a factor of two as final test concentration.
24.
Keep the dilution series
at 15 °C for at least 5
minutes to correct the
temperature.
Figure 7 Before the dilution series is started - 9 dilutions, D 2 and higher
1
Sample
2
2% NaCl solution
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