Hach Eclox, User Manual

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11.10.3 Prepare a 9 dilution series (D 1 values and higher)
To make a 9 dilution series according to ISO 11348 of D 1 sample values and higher,
do this procedure.
This procedure makes dilutions ranging from undiluted to a dilution ratio of 1:8 in
Row A. This corresponds to D values of 1 to 16 ( Figure 9 ) in the test, as 0.5 mL of
the sample dilution is added to 0.5 mL of the test suspension during the test in Row
B and C. Adding test suspension to the sample dilutions increases the sample
dilutions in row A by a factor of two as final test concentration.
Note: The test tubes in the higher Row A positions are more concentrated. The pipette is
moved from A9 to A4 (higher concentration to lower concentration) when making the dilution
series, so the pipette tip does not need to be replaced during this procedure.
Figure 9 Dilution series - 9 dilutions, D 1 values and higher
Tube Contents
A1 2% NaCl (2.0 mL)
A2 Sample (2.0 mL)
A3 2% NaCl (1.5 mL)
A4, A5 2% NaCl and sample (3.0 mL)
A6 - A9 2% NaCl and sample (1.5 mL)
A10 Sample (1.5 mL)