Hach Eclox, User Manual

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Solid NaCl is used to change the sample osmolarity to a value that is correct for
the marine bacterium used in the test.
4. If the sample has a high toxicity, carry out a preliminary dilution of the sample
with 2% NaCl solution. Select a preliminary dilution from the levels 1:2, 1:4, 1:8,
1:16, etc. to make sure of a continuous dilution series using the dilution
procedure of the manufacturer.
10.11 Prepare the test tubes
At the end of this procedure, the test tubes contain the percent sample dilutions
shown in Figure 3 .
Figure 3 Screening dilution series
1 Test suspension 3 Sample
2 2% NaCl solution
1. Put four test tubes in the
test tube stand.
2. Set the 0.2 - 1.0 mL
pipette to 0.2 mL.
3. Put the end of the pipette
into a clean pipette tip.