Dionex UltiMate FLM-3100, Operating Instructions Manual

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UltiMate 3000 Series: Flow Managers and Column Thermostats
Page 4 Operating Instructions
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Use only standard HPLC solvents (HPLC-grade: 0.2 µm filtered) and buffers that are
compatible with components in the flow path of the flow manager. Note the special
properties of the solvents such as viscosity, boiling point, UV absorption (UV/VIS
detector), and refractive index (refractive index detector).
Buffer concentration: typically up to 1 mol/L (0.1 mol/L if chloride ions are present).
•
After operation, rinse out buffers and solutions that form peroxides from your HPLC
system.
•
Before switching from buffer to organic solution, rinse the analytical system thoroughly
with deionized or HPLG-grade water.
•
When switching to another solvent, ensure that the new solvent is miscible with the one
contained in the pump. Otherwise, the analytical system can be damaged; for example, by
flocculation.
•
Avoid open flames and sparks, in particular if the mobile phase includes volatile or
flammable solvents.
•
If a leak occurs, turn off the flow manager immediately, stop the pump flow, and remedy
the situation.
•
When the front, side or rear panels are removed, dangerous electrical connections will be
exposed. Disconnect the flow manager from all power sources before removing the panels.
Remove panels only when instructed to do so in these instructions.
•
Always replace blown fuses with the original spare fuses from Dionex (
→
Replacing the
Fuses, page 73).
•
Replace faulty power cords and communication cables.
•
Many organic solvents and buffers are toxic. Know the toxicological properties of all
mobile phases that you are using.
•
If the toxicological properties of a sample are unknown to you, treat it as if it contains a
potentially harmful substance.
•
Wear goggles when handling mobile phases or operating the instrument. An eye wash
facility and a sink should be close to the unit. If any mobile phase splashes on the eyes or
skin, wash the affected areas with sufficient water and seek medical attention.
•
Dispose of all waste mobile phase in an environmentally safe manner that is consistent
with all local regulations. Do not allow flammable and/or toxic solvents to accumulate.
Follow a regulated, approved waste disposal program. Never dispose of flammable and/or
toxic solvents through the municipal sewage system